Novel Applications of Microextraction Techniques Focused on Biological and Forensic Analyses

In recent years, major attention has been focused on microextraction procedures that allow high recovery of target analytes, regardless of the complexity of the sample matrices. The most used techniques included liquid-liquid extraction (LLE), solid-phase extraction (SPE), solid-phase microextraction (SPME), dispersive liquid-liquid microextraction (DLLME), microextraction by packed sorbent (MEPS), and fabric-phase sorptive extraction (FPSE). These techniques manifest a rapid development of sample preparation techniques in different fields, such as biological, environmental, food sciences, natural products, forensic medicine, and toxicology. In the biological and forensic fields, where a wide variety of drugs with different chemical properties are analyzed, the sample preparation is required to make the sample suitable for the instrumental analysis, which often includes gas chromatography (GC) and liquid chromatography (LC) coupled with mass detectors or tandem mass detectors (MS/MS). In this review, we have focused our attention on the biological and forensic application of these innovative procedures, highlighting the major advantages and results that have been accomplished in laboratory and clinical practice.

Revaccination with Bacille Calmette-Guérin (BCG) is associated with an increased risk of abscess and lymphadenopathy

The reported frequency and types of adverse events following initial vaccination and revaccination with Bacille Calmette-Guérin (BCG) varies worldwide. Using active surveillance in a randomised controlled trial of BCG vaccination (the BRACE trial), we determined the incidence and risk factors for the development of BCG injection site abscess and regional lymphadenopathy. Injection site abscess occurred in 3% of 1387 BCG-vaccinated participants; the majority (34/41, 83%) resolved without treatment. The rate was higher in BCG-revaccinated participants (OR 3.6, 95% CI 1.7–7.5), in whom abscess onset was also earlier (median 16 vs. 27 days, p = 0.008). No participant with an abscess had a positive interferon-gamma release assay. Regional lymphadenopathy occurred in 48/1387 (3%) of BCG-vaccinated participants, with a higher rate in revaccinated participants (OR 2.1, 95% CI 1.1–3.9). BCG-associated lymphadenopathy, but not injection site abscess, was influenced by age and sex. A previous positive tuberculin skin test was not associated with local reactions. The increased risk of injection site abscess or lymphadenopathy following BCG revaccination is relevant to BCG vaccination policy in an era when BCG is increasingly being considered for novel applications.

Current findings and future prospective of high-value trans Himalayan medicinal plant Lycium ruthenicum Murr: a systematic review

Background The genus Lycium is commercially known for its nutrient dense goji-berries, among these berries, black goji-berries obtained from Lycium ruthenicum Murr are highly valued and widely used as traditional medicine in trans-himalayan cold desert Ladakh and as functional food in several countries. Methods The current collection of data and literature was done by exploring different scientific portals like SciFinder, Google scholar, PubMed, Dictonary of Natural Products, Institute for Scientific Information, Web of Science and Scopus by searching keywords like black goji berry, crystal pearl, and trans-Himalayan plant. Results Fruits of L. ruthenicum Murr, are overwhelmingly enriched in anthocyanins, proanthocyanidins, polysaccharides, spermine and spermidine alkaloids. The presence of these bioactive phyto-chemicals has been linked with reported anti-diabetic, anti-inflammatory, anti-fatigue, anti-atherosclerosis and neuro-protective properties of black goji berries. A unique color of these berries makes them exceptional as compared to other berries. Conclusions In this article, we have reviewed the variety of high value phytochemicals of Lycium ruthenicum Murr, with a special focus on health promoting anthocyanins which will provide an insight to the readers for exploring novel applications of L. ruthenicum Murr in field of medicine and food industries.

Atomic-Scale Characterization of Structural Heterogeny in 2D TMD Layers

The heterogeneity features among 2-dimensional (2D) transition metal dichalcogenides (TMDs) layered materials endow them with distinctive properties for a vast array of novel applications. Their unique properties stem from the...

Catalytic nanoparticles and magnetic nanocatalysts in organic reactions: A mini review

Nanocatalysts, as a part of nanotechnology, have been seen very useful for various fileds of applications capturing a large contribution of the world market. Indeed, several unsolved issues of catalysts have been reconsidered by employing the new nanocatalysts including single core metal atoms and ions with surrounding holes. Moreover, it was expected that the future of catalytic reactions, especially those organic ones, will deal with the nanocatalyst applications. To this aim, the features of catalytic nanoparticles and magnetic nanocatalysts regarding evaluation of their advantages and applications in organic reactions were investigated in this work. Developments of catalytic nanoparticles and magnetic nanocatalysts were discussed in this work regarding the novel applications of such materials at the nanoscale for approaching advantageous features. Increased availability, activity, and stability are very important for applications of the catalysts in various organic reactions. Therefore, it is a must to discuss features of such nanocatalytic systems to provide more information about their advantages and even disadvantages of their applications.

Cyclodextrins: Structural, Chemical, and Physical Properties, and Applications

Due to their unique structural, physical and chemical properties, cyclodextrins and their derivatives have been of great interest to scientists and researchers in both academia and industry for over a century. Many of the industrial applications of cyclodextrins have arisen from their ability to encapsulate, either partially or fully, other molecules, especially organic compounds. Cyclodextrins are non-toxic oligopolymers of glucose that help to increase the solubility of organic compounds with poor aqueous solubility, can mask odors from foul-smelling compounds, and have been widely studied in the area of drug delivery. In this review, we explore the structural and chemical properties of cyclodextrins that give rise to this encapsulation (i.e., the formation of inclusion complexes) ability. This review is unique from others written on this subject because it provides powerful insights into factors that affect cyclodextrin encapsulation. It also examines these insights in great detail. Later, we provide an overview of some industrial applications of cyclodextrins, while emphasizing the role of encapsulation in these applications. We strongly believe that cyclodextrins will continue to garner interest from scientists for many years to come, and that novel applications of cyclodextrins have yet to be discovered.

Generation and characterization of complex vector modes with digital micromirror devices: a tutorial

Complex vector light modes with a spatial variant polarization distribution have become topical of late, enabling the development of novel applications in numerous research fields. Key to this is the remarkable similarities they hold with quantum entangled states, which arises from the non-separability between the spatial and polarisation degrees of freedom (DoF). As such, the demand for diversification of generation methods and characterization techniques have increased dramatically. Here we put forward a comprehensive tutorial about the use of DMDs in the generation and characterization of vector modes, providing details on the implementation of techniques that fully exploits the unsurpassed advantage of Digital Micromirrors Devices (DMDs), such as their high refresh rates and polarisation independence. We start by briefly describing the operating principles of DMD and follow with a thorough explanation of some of the methods to shape arbitrary vector modes. Finally, we describe some techniques aiming at the real-time characterization of vector beams. This tutorial highlights the value of DMDs as an alternative tool for the generation and characterization of complex vector light fields, of great relevance in a wide variety of applications.