Discrepant equations derived from the single radial immunodiffusion technique

1972 ◽  
Vol 9 (12) ◽  
pp. 1256-1259
Author(s):  
B Berne
Keyword(s):  
Radial Immunodiffusion ◽  
Single Radial Immunodiffusion

Single radial immunodiffusion of serum apolipoproteins C-II, C-III and E — pretreatment of samples with surfactant

1986 ◽  
Vol 161 (3) ◽  
pp. 275-282 ◽  
Author(s):  
Koji Itakura ◽  
Takeyasu Matsudate ◽  
Takato Sakurai ◽  
Shunichi Hashimoto ◽  
Kazue Ito ◽  
...  
Keyword(s):  
Radial Immunodiffusion ◽  
Single Radial Immunodiffusion

Complement C3 in serum and plasma, as measured by radial immunodiffusion with four commercial kits.

1976 ◽  
Vol 22 (2) ◽  
pp. 267-269 ◽  
Author(s):  
A O Vladutiu ◽  
B M Winiarski
Keyword(s):  
Radial Immunodiffusion ◽  
Complement C3 ◽  
Antigenic Determinants ◽  
Sample Processing ◽  
Single Radial Immunodiffusion ◽  
Normal Individuals ◽  
Commercial Kits

Abstract Quantitation of the C3 component of complement by single radial immunodiffusion is subject to error because the C3 molecule has several antigenic determinants and anti-C3 sera differ in their specificity to these determinants. C3 was measured in plasma (ethylenediaminetetraacetate anticoagulant) and serum, in five normal individuals, by use of four such commercial kits. The effects of ethylenediaminetetraacetate and of incubation at -20, 4, and 37 degrees C for 1, 2, and 7 days were investigated. We found wide variations in C3 in the same sample, as measured with different kits. Incubation for longer than 48 h at 4 degrees C changed C3 concentrations as compared to those in fresh samples. Reference sera for C3 assay and standardized procedures for sample processing are both essential to valid results.

Evaluation of Single Radial Immunodiffusion for Quantitation of Plasma Fibrinogen

1972 ◽  
Vol 57 (1) ◽  
pp. 89-94 ◽  
Author(s):  
G. M. Brittin ◽  
H. Rafinia ◽  
D. Raval ◽  
M. Werner ◽  
D. Brown
Keyword(s):  
Plasma Fibrinogen ◽  
Radial Immunodiffusion ◽  
Single Radial Immunodiffusion
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