Site-specific cleavage of unusual DNA and RNA structures by rhodium & ruthenium complexes

1993 ◽  
Vol 51 (1-2) ◽  
pp. 529
Author(s):  
Ai Ching Lim ◽  
Jacqueline K. Barton
Keyword(s):  
Ruthenium Complexes ◽  
Rna Structures ◽  
Site Specific ◽  
Dna And Rna

scholarly journals Effects of site-specific substitution of 5-fluorouridine on the stabilities of duplex DNA and RNA

1995 ◽  
Vol 23 (19) ◽  
pp. 3916-3921 ◽  
Author(s):  
Parag V. Sahasrabudhe ◽  
Richard T. Pon ◽  
William H. Gmeiner
Keyword(s):  
Duplex Dna ◽  
Site Specific ◽  
Dna And Rna

scholarly journals Evolving state grammar for modeling DNA and RNA structures

2017 ◽  
Vol 7 (1) ◽  
pp. 19 ◽  
Author(s):  
Ajay Kumar ◽  
Nidhi Kalra ◽  
Sunita Garhwal
Keyword(s):  
Molecular Structures ◽  
Type Structure ◽  
Rna Structures ◽  
Production Rules ◽  
Pseudoknot Structure ◽  
Descriptional Complexity ◽  
Dna And Rna ◽  
Pushdown Automata ◽  
State Grammar

In this paper, we represent bio-molecular structures (Attenuator, Extended Pseudoknot Structure, Kissing Hairpin, Simple H-type structure, Recursive Pseudoknot and Three-knot Structure) using state grammar. These representations will be measured using descriptional complexity point of views. Results indicate that the proposed approach is more succinct in terms of production rules and variables over the existing approaches. Another major advantage of the proposed approach is state grammar can be represented by deep pushdown automata, whereas no such automaton exists for matrix ins-del system.

scholarly journals Site-Specific Labeling of DNA and RNA Using an Efficiently Replicated and Transcribed Class of Unnatural Base Pairs

2011 ◽  
Vol 133 (49) ◽  
pp. 19878-19888 ◽  
Author(s):  
Young Jun Seo ◽  
Denis A. Malyshev ◽  
Thomas Lavergne ◽  
Phillip Ordoukhanian ◽  
Floyd E. Romesberg
Keyword(s):  
Base Pairs ◽  
Site Specific ◽  
Dna And Rna

scholarly journals Exploring DNA and RNA Structures in Solution with Infrared Spectroscopy

2017 ◽  
Vol 112 (3) ◽  
pp. 474a
Author(s):  
Allison L. Stelling ◽  
Hashim Al-Hashimi
Keyword(s):  
Infrared Spectroscopy ◽  
Rna Structures ◽  
Dna And Rna

Prediction and design of DNA and RNA structures

2010 ◽  
Vol 27 (3) ◽  
pp. 184-193 ◽  
Author(s):  
Ebbe Sloth Andersen
Keyword(s):  
Rna Structures ◽  
Dna And Rna

scholarly journals Dual-gRNA Approach With Limited Off-Target Effect Corrects C9ORF72 Repeat Expansion In Vivo

2021 ◽  
Author(s):  
Xuejiao Piao ◽  
Dawei Meng ◽  
Xue Zhang ◽  
Qiang Song ◽  
Hailong Lv ◽  
...  
Keyword(s):  
Low Complexity ◽  
Repeat Expansion ◽  
Concept Design ◽  
Rna Structures ◽  
Dna And Rna ◽  
Therapeutic Value ◽  
Repeat Dna ◽  
Target Effect

Abstract C9ORF72 GGGGCC repeat expansion is the most common genetic cause for amyotrophic lateral sclerosis and frontotemporal dementia, which generates abnormal DNA and RNA structures and produces toxic proteins. Recently, efficacy of CRISPR/Cas9-mediated editing has been proven in treatment of disease. However, DNA low complexity surrounding C9ORF72 expansion increases the off-target risks. Here we provide a dual-gRNA design outside of the low complexity region which enables us to remove the repeat DNA in a ‘cutting-deletion-fusion’ manner with a high fusion efficiency (50%). Our dual-gRNA design limits off-target effect and does not significantly affect C9ORF72 expression. In neurons carrying patient C9ORF72 expansion, our approach removes the repeat DNA and corrects the RNA foci in vitro and in vivo. Therefore, we conclude that our proof-of-concept design correct C9ORF72 repeat expansion, which may have potential therapeutic value for the patients.

An unnatural hydrophobic base pair system: site-specific incorporation of nucleotide analogs into DNA and RNA

2006 ◽  
Vol 3 (9) ◽  
pp. 729-735 ◽  
Author(s):  
Ichiro Hirao ◽  
Michiko Kimoto ◽  
Tsuneo Mitsui ◽  
Tsuyoshi Fujiwara ◽  
Rie Kawai ◽  
...  
Keyword(s):  
Base Pair ◽  
Nucleotide Analogs ◽  
Site Specific ◽  
Dna And Rna
Sign in / Sign up

Export Citation Format

Share Document