A simple test system (umu test) for the detection of environmental mutagens. II. Improvement of sensitivity by employing Salmonella typhimurium TA1535 as a host strain

1984 ◽  
Vol 130 (5) ◽  
pp. 375 ◽  
Author(s):  
Y. Oda ◽  
S. Nakamura ◽  
I. Oki ◽  
K. Sugita ◽  
T. Kato ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Test System ◽  
Host Strain ◽  
Simple Test ◽  
Umu Test

scholarly journals A new Salmonella typhimurium NM5004 strain expressing rat glutathione S-transferase 5–5: use in detection of genotoxicity of dihaloalkanes using an SOS/umu test system

1996 ◽  
Vol 17 (2) ◽  
pp. 297-302 ◽  
Author(s):  
Yoshimitsu Oda ◽  
Hiroshi Yamazaki ◽  
Ricarda Thier ◽  
Brian Ketterer ◽  
F.Peter Guengerich ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Test System ◽  
Glutathione S Transferase ◽  
Umu Test

Evaluation of SOS-inducing activity with an O-acetyltransferase-overexpressing strain Salmonella typhimurium NM2009 for municipal river water and the identification of 1-nitropyrene

1996 ◽  
Vol 33 (6) ◽  
pp. 313-320 ◽  
Author(s):  
Takeshi Ohe
Keyword(s):  
Salmonella Typhimurium ◽  
River Water ◽  
Wastewater Treatment Plants ◽  
Test System ◽  
Umu Test ◽  
Highly Sensitive ◽  
Organic Extracts ◽  
Direct Acting ◽  
Overexpressing Strain ◽  
Blue Rayon

Evaluation of SOS-inducing activity of organic extracts from tributaries of the Yodo River in Kyoto was examined with a highly sensitive umu test system using an O -acetyltransferase(O -ATase)-overexpressing strain Salmonella typhimurium NM2009 and an O-ATase-defective strain NM2000. Organic extracts in river water were collected by the XAD-2 resin column method and the blue rayon hanging method at 4 locations throughout the year. Both XAD-2 resin and blue rayon extracts collected at downstream locations from wastewater treatment plants showed higher SOS-inducing activity than those at the upsream location in the absence and the presence of S9 mix with NM2009. It was also shown that the constituents contributing SOS-inducing activity were different between XAD-2 extract and blue rayon extract. No or weak responses of SOS-inducing activity were observed with an O -ATase-defective strain NM2000 among all extracts performed. These results indicate that a highly sensitive umu test using an O-ATase-overexpressing strain and an O-ATase-defective strain proved to be an excellent tool for the monitoring of genotoxicity attributable to trace amounts of genotoxicants such as direct-acting nitroarenes and/or indirect-acting aromatic amines in river water. Further study was made to identify 1-nitropyrene in XAD-2 resin extract from municipal river water.

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