The orientation of phosphate activated glutaminase in the inner mitochondrial membrane of synaptic and non-synaptic rat brain mitochondria

1995 ◽  
Vol 27 (4-5) ◽  
pp. 367-376 ◽  
Author(s):  
B Roberg
Keyword(s):  
Rat Brain ◽  
Mitochondrial Membrane ◽  
Brain Mitochondria ◽  
Inner Mitochondrial Membrane ◽  
Rat Brain Mitochondria ◽  
Phosphate Activated Glutaminase

scholarly journals Glutamate and aspartate transport in rat brain mitochondria

1974 ◽  
Vol 140 (2) ◽  
pp. 205-210 ◽  
Author(s):  
M. D. Brand ◽  
J. B. Chappell
Keyword(s):  
Rat Brain ◽  
Mitochondrial Membrane ◽  
Liver Mitochondria ◽  
Brain Mitochondria ◽  
Kidney Mitochondria ◽  
Pig Kidney ◽  
Rat Brain Mitochondria ◽  
Energy Dependent ◽  
Glutaminase Activity ◽  
Malate Aspartate Shuttle

1. Rat brain mitochondria did not swell in iso-osmotic solutions of ammonium or potassium (plus valinomycin) glutamate or aspartate, with or without addition of uncouplers. 2. Glutamate was able to reduce intramitochondrial NAD(P)+; aspartate was able to cause partial re-oxidation. 3. These effects were inhibited by threo-hydroxy-aspartate in whole but not in lysed mitochondria. 4. The existence of a ‘malate–aspartate shuttle’ for the oxidation of extramitochondrial NADH was demonstrated. This shuttle requires the net exchange of glutamate for aspartate across the mitochondrial membrane. 5. Extramitochondrial glutamate did not inhibit intramitochondrial glutaminase under conditions in which the inhibition in lysed mitochondria was virtually complete. 6. The glutaminase activity of these mitochondria was not energy-dependent. 7. We conclude that these mitochondria do not possess a glutamate–hydroxyl antiporter similar to that of liver mitochondria nor a glutamate–glutamine antiporter similar to that of pig kidney mitochondria, but that they do possess a glutamate–aspartate antiporter.

Acyl-CoA: sn-Glycerol-3-Phosphate O-Acyltransferase in Rat Brain Mitochondria and Microsomes

1982 ◽  
Vol 39 (1) ◽  
pp. 286-289 ◽  
Author(s):  
Susan M. Fitzpatrick ◽  
Giovanna Sorresso ◽  
Dipak Haldar
Keyword(s):  
Rat Brain ◽  
Brain Mitochondria ◽  
Rat Brain Mitochondria
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