Studies on the CoQH2-cytochrome c reductase segment of the respiratory chain of yeast mitochondria, using mutants of the cytochrome b split gene

Biochimie ◽  
1989 ◽  
Vol 71 (11-12) ◽  
pp. 1145-1155 ◽  
Author(s):  
Danielle Lemesle-Meunier
Keyword(s):  
Cytochrome C ◽  
Cytochrome B ◽  
Respiratory Chain ◽  
Yeast Mitochondria ◽  
Cytochrome C Reductase ◽  
Split Gene

A mitochondrial cytochrome b mutation but no mutations of nuclearly encoded subunits in ubiquinol cytochrome c reductase (complex III) deficiency

1999 ◽  
Vol 104 (6) ◽  
pp. 460-466 ◽  
Author(s):  
Isabelle Valnot ◽  
Johanna Kassis ◽  
Dominique Chretien ◽  
Pascale de Lonlay ◽  
Béatrice Parfait ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Cytochrome B ◽  
Complex Iii ◽  
Mitochondrial Cytochrome ◽  
Cytochrome C Reductase ◽  
Mitochondrial Cytochrome B

scholarly journals Oxygen affinity of the respiratory chain of Acanthamoeba castellanii

1983 ◽  
Vol 214 (1) ◽  
pp. 47-51 ◽  
Author(s):  
D Lloyd ◽  
H Mellor ◽  
J L Williams
Keyword(s):  
Plasma Membrane ◽  
Cytochrome C ◽  
Cytochrome B ◽  
Respiratory Chain ◽  
Oxygen Affinity ◽  
Acanthamoeba Castellanii ◽  
Intact Cells ◽  
Cytochrome Aa3 ◽  
Isolated Mitochondria ◽  
Soil Amoeba

Apparent Km values for O2 for the soil amoeba Acanthamoeba castellanii determined polarographically and by bioluminescence gave similar values (0.37 and 0.41 microM respectively). Mitochondria oxidizing succinate or NADH in the presence or absence of ADP gave values in the range 0.21-0.36 microM-O2. Oxidation of respiratory-chain components to 50% of the aerobic steady states in intact cells was observed at the following O2 concentrations: cytochrome aa3, 0.1-0.25 microM; cytochrome c, 0.3-0.6 microM; cytochrome b, 0.35-0.45 microM; flavoprotein, 2 microM. In isolated mitochondria corresponding values for a-, c- and b-type cytochromes were 0.007, 0.035-0.05 and 0.06-0.09 microM-O2. It is concluded that an O2 gradient exists between plasma membrane and mitochondria in A. castellanii.

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