Metabolic effects of acetate in perfused rat liver studies on ketogenesis, glucose output, lactate uptake and lipogenesis

1982 ◽  
Vol 716 (3) ◽  
pp. 290-297 ◽  
Author(s):  
Alan M. Snoswell ◽  
Rodney P. Trimble ◽  
Richard C. Fishlock ◽  
Gerald B. Storer ◽  
David L. Topping
Keyword(s):  
Rat Liver ◽  
Metabolic Effects ◽  
Perfused Rat Liver ◽  
Lactate Uptake ◽  
Glucose Output

Coordinated regulation of hepatic glycogen formation in perfused rat liver by glucose and lactate

1994 ◽  
Vol 266 (4) ◽  
pp. E583-E591 ◽  
Author(s):  
Z. Zhang ◽  
J. Radziuk
Keyword(s):  
Rat Liver ◽  
Dose Response ◽  
Glycogen Synthesis ◽  
Hepatic Glycogen ◽  
Perfused Rat Liver ◽  
Response Curves ◽  
Liver Preparation ◽  
Lactate Uptake ◽  
Glucose Output ◽  
Glycogen Formation

Lactate has been found to enhance the formation of glycogen from both glucose and lactate as substrate (Z. Zhang and J. Radziuk. Biochem. J. 280: 415–419, 1991). To evaluate the relative importance of its role as substrate and regulatory factor, a dual dose-response evaluation was done by adding variable amounts of glucose and lactate to the medium in a recirculating perfused rat liver preparation. Nine groups of perfusions were performed utilizing three different levels of carbon infusion into the system: 0.25, 1.0, and 2.0 mg/min. These levels of carbon infusion were further subdivided into different relative amounts of glucose and lactate. Lactate uptake by the perfused liver was linearly related with net glucose output, regardless of the glucose concentrations. In contrast to this, the effect of lactate uptake on the rate of glycogen synthesis is saturable. Moreover, the rate of glycogen formation at which this saturation occurs is dependent only on the mean perfusate glucose concentration. The highest amount of glycogen formed in a 2-h period was 50 +/- 7 mg and the lowest 3.4 +/- 0.3 mg. A family of dose-response curves was generated describing this dual dependence of glycogen formation (both direct and gluconeogenetic pathways) on lactate and glucose.

The Effect of Combined Ischaemia and Acidosis on Lactate Uptake and Gluconeogenesis in the Perfused Rat Liver

1981 ◽  
Vol 60 (5) ◽  
pp. 537-542 ◽  
Author(s):  
R. A. Iles ◽  
R. D. Cohen ◽  
P. G. Baron
Keyword(s):  
Lactic Acid ◽  
Rat Liver ◽  
Hepatic Uptake ◽  
Strenuous Exercise ◽  
Perfused Rat Liver ◽  
Lactate Uptake ◽  
Glucose Output ◽  
Rat Livers

1. Perfused rat livers were subjected to an acid perfusate and varying degrees of ischaemia in an attempt to simulate the conditions of strenuous exercise or shock. 2. Lactate uptake and glucose output from the liver decreased during moderate ischaemia alone and more so when, in addition, the perfusate was made acidic. 3. Hepatic ATP and ADP content increased in the presence of an acid perfusate. 4. It is concluded that both ischaemia and acidosis may contribute to the diminished hepatic uptake of lactic acid in strenuous exercise and shock.

scholarly journals Metabolic Effects of Ethanol in Perfused Rat Liver

1969 ◽  
Vol 244 (18) ◽  
pp. 5044-5054 ◽  
Author(s):  
J R Williamson ◽  
R Scholz ◽  
E T Browning ◽  
R G Thurman ◽  
M H Fukami
Keyword(s):  
Rat Liver ◽  
Metabolic Effects ◽  
Perfused Rat Liver

Vanadate inhibits glucose output from isolated perfused rat liver

Hepatology ◽  
1991 ◽  
Vol 14 (3) ◽  
pp. 540-544 ◽  
Author(s):  
Rafael Bruck ◽  
Haia Prigozin ◽  
Zipora Krepel ◽  
Paul Rotenberg ◽  
Yoram Shechter ◽  
...  
Keyword(s):  
Rat Liver ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Glucose Output

The Effect of Simulated Metabolic Acidosis on Intracellular pH and Lactate Metabolism in the Isolated Perfused Rat Liver

1973 ◽  
Vol 45 (4) ◽  
pp. 543-549 ◽  
Author(s):  
M. H. Lloyd ◽  
R. A. Iles ◽  
B. R. Simpson ◽  
J. M. Strunin ◽  
J. M. Layton ◽  
...  
Keyword(s):  
Metabolic Acidosis ◽  
Intracellular Ph ◽  
Rat Liver ◽  
Extracellular Ph ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Lactate Metabolism ◽  
Lactate Output ◽  
Lactate Uptake ◽  
The Relationship

1. The relationship between extracellular pH (pHe), intracellular pH (pHi) and lactate uptake was studied in the isolated perfused rat liver during simulated metabolic acidosis. 2. pHi fell to a considerably less extent than pHe when the latter was decreased from pH 7·4 to 6·7. 3. The liver took up lactate when pHi was greater than 7·0; at lower values of pHi lactate output occurred. 4. The relevance of these observations to the control of hepatic pHi and lactate metabolism is discussed.

Glyburide Sensitizes Perfused Rat Liver to Insulin-Induced Suppression of Glucose Output

Diabetes ◽  
1987 ◽  
Vol 36 (4) ◽  
pp. 472-476 ◽  
Author(s):  
O. P. McGuinness ◽  
D. R. Green ◽  
A. D. Cherrington
Keyword(s):  
Rat Liver ◽  
Perfused Rat Liver ◽  
Glucose Output

Metabolic effects ofp-coumaric acid in the perfused rat liver

2006 ◽  
Vol 20 (1) ◽  
pp. 18-26 ◽  
Author(s):  
Leonardo C.N. Lima ◽  
Gisele D. Buss ◽  
Emy L. Ishii-Iwamoto ◽  
Clairce Salgueiro-Pagadigorria ◽  
Jurandir Fernando Comar ◽  
...  
Keyword(s):  
Rat Liver ◽  
Metabolic Effects ◽  
Perfused Rat Liver ◽  
Coumaric Acid

Effect of Varying Pco2 on Intracellular pH and Lactate Consumption in the Isolated Perfused Rat Liver

1978 ◽  
Vol 55 (2) ◽  
pp. 175-181 ◽  
Author(s):  
P. G. Baron ◽  
R. A. Iles ◽  
R. D. Cohen
Keyword(s):  
Intracellular Ph ◽  
Rat Liver ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Lactate Metabolism ◽  
Isolated Rat Liver ◽  
Buffering Effect ◽  
Lactate Uptake ◽  
Lactate Consumption ◽  
Isolated Rat

1. The effects of varying Pco2 on lactate uptake and intracellular pH (pHl) were studied in the isolated rat liver perfused with differing concentrations of lactate. 2. In general, pHl and lactate uptake are inversely related to Pco2, and pHl and lactate uptake are directly related to each other, but the quantitative aspects and significance of these relationships vary with the availability of lactate. A model of hepatic lactate metabolism is proposed which may account for the quantitative variation. 3. The metabolism of lactate within the hepatocyte exerts a destabilizing effect on hepatocyte cell pH, in contrast to the buffering effect seen in predominantly glycolytic tissues. 4. An attempt is made to relate the findings to the disturbances of lactate metabolism in clinical respiratory failure.

Production, Uptake, and Metabolic Effects of Cyclic AMP in the Bivascularly Perfused Rat Liver

1997 ◽  
Vol 54 (10) ◽  
pp. 1115-1125 ◽  
Author(s):  
Jorgete Constantin ◽  
Fumie Suzuki-Kemmelmeier ◽  
Nair Seiko Yamamoto ◽  
Adelar Bracht
Keyword(s):  
Cyclic Amp ◽  
Rat Liver ◽  
Metabolic Effects ◽  
Perfused Rat Liver
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