Localization of vasopressin binding sites in rat brain by in vitro autoradiography using a radioiodinated V1 receptor antagonist

N-methyl-D-aspartate (NMDA) receptors are known to play an important role in learning and memory and to be involved in neuron cell death accompanying cerebral ischemia, seizures, and Alzheimer's disease. The NMDA receptor complex has been considered to consist of an L-glutamate recognition site, a strychnine-insensitive glycine modulatory site, and a voltage-dependent cation channel. In the present study, effects of age on an L-glutamate recognition site and a glycine site were examined in rat brain by quantitative in vitro autoradiography with [3H]-CPP and [3H]-glycine. Both [3H]-glycine and [3H]-CPP binding sites were most abundant in the hippocampus and cerebral cortex, and they showed a similar distribution pattern throughout the brain. [3H]-glycine binding sites were severely decreased in the telencephalic regions, including the hippocampus and cerebral cortex, in aged brain. Conversely, [3H]-CPP binding sites were well preserved in these brain areas. In the mid-brain regions and cerebellum, neither [3H]-glycine nor [3H]-CPP binding sites changed in the aged brain. Our results indicate that within the NMDA receptor complex, glycine receptors are primarily affected in the aging process.

Download Full-text

Absence of age-related changes in 3H-AMPA binding sites in rat brain studied by quantitative in vitro autoradiography

The Japanese Journal of Pharmacology ◽

10.1016/s0021-5198(19)55897-8 ◽

1990 ◽

Vol 52 ◽

pp. 358

Author(s):

Rie Miyoshi ◽

Shozo Kito ◽

Naomi Doudou

Keyword(s):

Rat Brain ◽

Binding Sites ◽

In Vitro Autoradiography ◽

Age Related ◽

Age Related Changes

Download Full-text

Localization of binding sites for calcitonin gene-related peptide in rat brain by in vitro autoradiography

Neuroscience ◽

10.1016/0306-4522(86)90137-5 ◽

1986 ◽

Vol 19 (4) ◽

pp. 1235-1245 ◽

Cited By ~ 86

Author(s):

P.M. Sexton ◽

J.S. McKenzie ◽

R.T. Mason ◽

J.M. moseley ◽

T.J. Martin ◽

...

Keyword(s):

Rat Brain ◽

Binding Sites ◽

Calcitonin Gene Related Peptide ◽

In Vitro Autoradiography ◽

Related Peptide ◽

Calcitonin Gene

Download Full-text

Melatonin-binding sites in the rat brain and pituitary mapped by in-vitro autoradiography

Journal of Molecular Endocrinology ◽

10.1677/jme.0.0030071 ◽

1989 ◽

Vol 3 (1) ◽

pp. 71-75 ◽

Cited By ~ 56

Author(s):

L. M. Williams

Keyword(s):

Rat Brain ◽

Binding Sites ◽

Area Postrema ◽

Specific Binding ◽

Melatonin Receptors ◽

Pars Tuberalis ◽

Lower Affinity ◽

High Affinity ◽

In Vitro Autoradiography

ABSTRACT Using picomolar concentrations of [125I]iodomelatonin and in-vitro autoradiography, specific melatonin-binding sites have been mapped in the rat brain and pituitary. Using this same technique, high-affinity melatonin receptors had previously been identified in the suprachiasmatic nucleus (SCN) and median eminence regions of the rat hypothalamus. The presence of melatonin binding in the SCN has been confirmed, but the second area of binding has been identified as the pars tuberalis of the pituitary, and a completely novel area of binding is also reported in the area postrema. The existence of lower affinity melatonin receptors in the rat brain was also investigated using in-vitro autoradiography and higher concentrations of [125I]iodomelatonin. No further sites of specific binding were, however, disclosed.

Download Full-text