Studies on the organization of the α-satellite DNA from African green monkey cells using restriction nucleases and molecular cloning

Gene ◽  
1979 ◽  
Vol 5 (2) ◽  
pp. 93-110 ◽  
Author(s):  
Hermann Graf ◽  
Friedrich Fittler ◽  
Hans G. Zachau
Keyword(s):  
Molecular Cloning ◽  
Satellite Dna ◽  
African Green Monkey ◽  
Green Monkey ◽  
African Green Monkey Cells

scholarly journals UV Irradiation induces an activity which stimulates Simian virus 40 rescue upon cell fusion.

1984 ◽  
Vol 4 (6) ◽  
pp. 1159-1162 ◽  
Author(s):  
S Nomura ◽  
M Oishi
Keyword(s):  
Cell Fusion ◽  
Uv Irradiation ◽  
Syrian Hamster ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
African Green Monkey ◽  
Green Monkey ◽  
African Green Monkey Cells ◽  
Stimulation Of

UV irradiation of African green monkey cells greatly stimulated efficiency of simian virus 40 induction from simian virus 40-transformed Syrian hamster cells after cell fusion. The maximum inducing activity was observed at 15 to 20 h after irradiation but remained only transiently. The addition of cycloheximide after UV irradiation eliminated the stimulation of the activity.

UV Irradiation induces an activity which stimulates Simian virus 40 rescue upon cell fusion

1984 ◽  
Vol 4 (6) ◽  
pp. 1159-1162
Author(s):  
S Nomura ◽  
M Oishi
Keyword(s):  
Cell Fusion ◽  
Uv Irradiation ◽  
Syrian Hamster ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
African Green Monkey ◽  
Green Monkey ◽  
African Green Monkey Cells ◽  
Stimulation Of

UV irradiation of African green monkey cells greatly stimulated efficiency of simian virus 40 induction from simian virus 40-transformed Syrian hamster cells after cell fusion. The maximum inducing activity was observed at 15 to 20 h after irradiation but remained only transiently. The addition of cycloheximide after UV irradiation eliminated the stimulation of the activity.

scholarly journals alpha DNA in African green monkey cells is organized into extremely long tandem arrays.

1986 ◽  
Vol 261 (5) ◽  
pp. 2314-2318
Author(s):  
H D Madhani ◽  
S A Leadon ◽  
C A Smith ◽  
P C Hanawalt
Keyword(s):  
African Green Monkey ◽  
Green Monkey ◽  
African Green Monkey Cells ◽  
Tandem Arrays

scholarly journals Surprising deficiency of CENP-B binding sites in African green monkey alpha-satellite DNA: implications for CENP-B function at centromeres.

1996 ◽  
Vol 16 (9) ◽  
pp. 5156-5168 ◽  
Author(s):  
I G Goldberg ◽  
H Sawhney ◽  
A F Pluta ◽  
P E Warburton ◽  
W C Earnshaw
Keyword(s):  
Binding Site ◽  
Satellite Dna ◽  
Binding Sites ◽  
Present Report ◽  
African Green Monkey ◽  
Dna Arrays ◽  
Alpha Satellite ◽  
Mobility Shift ◽  
Alpha Satellite Dna ◽  
Green Monkey

Centromeres of mammalian chromosomes are rich in repetitive DNAs that are packaged into specialized nucleoprotein structures called heterochromatin. In humans, the major centromeric repetitive DNA, alpha-satellite DNA, has been extensively sequenced and shown to contain binding sites for CENP-B, an 80-kDa centromeric autoantigen. The present report reveals that African green monkey (AGM) cells, which contain extensive alpha-satellite arrays at centromeres, appear to lack the well-characterized CENP-B binding site (the CENP-B box). We show that AGM cells express a functional CENP-B homolog that binds to the CENP-B box and is recognized by several independent anti-CENP-B antibodies. However, three independent assays fail to reveal CENP-B binding sites in AGM DNA. Methods used include a gel mobility shift competition assay using purified AGM alpha-satellite, a novel kinetic electrophoretic mobility shift assay competition protocol using bulk genomic DNA, and bulk sequencing of 76 AGM alpha-satellite monomers. Immunofluorescence studies reveal the presence of significant levels of CENP-B antigen dispersed diffusely throughout the nuclei of interphase cells. These experiments reveal a paradox. CENP-B is highly conserved among mammals, yet its DNA binding site is conserved in human and mouse genomes but not in the AGM genome. One interpretation of these findings is that the role of CENP-B may be in the maintenance and/or organization of centromeric satellite DNA arrays rather than a more direct involvement in centromere structure.

Sign in / Sign up

Export Citation Format

Share Document