Recognition sequence for the restriction endonuclease BglI from Bacillus globigii

Gene ◽  
1980 ◽  
Vol 9 (3-4) ◽  
pp. 195-203 ◽  
Author(s):  
Hugo Van Heuverswyn ◽  
Walter Fiers
Keyword(s):  
Restriction Endonuclease ◽  
Recognition Sequence

scholarly journals Crystal structure of restriction endonuclease BglI bound to its interrupted DNA recognition sequence

1998 ◽  
Vol 17 (18) ◽  
pp. 5466-5476 ◽  
Author(s):  
Matthew Newman ◽  
Keith Lunnen ◽  
Geoffrey Wilson ◽  
John Greci ◽  
Ira Schildkraut ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Restriction Endonuclease ◽  
Dna Recognition ◽  
Recognition Sequence

The effect of methylation outside the recognition sequence of restriction endonuclease PvuII on its cleavage efficiency

Gene ◽  
1992 ◽  
Vol 113 (1) ◽  
pp. 89-93 ◽  
Author(s):  
Qiang Liu ◽  
Xiongwei Chen ◽  
Xilin Zhao ◽  
Yawen Chen ◽  
Defeng Chen
Keyword(s):  
Restriction Endonuclease ◽  
Recognition Sequence ◽  
Cleavage Efficiency

Characterization of the Specific DNA Nicking Activity of Restriction Endonuclease N.BstNBI

2000 ◽  
Vol 381 (11) ◽  
pp. 1123-1125 ◽  
Author(s):  
R.D. Morgan ◽  
C. Calvet ◽  
M. Demeter ◽  
R. Agra ◽  
H. Kong
Keyword(s):  
Restriction Endonuclease ◽  
Cleavage Site ◽  
Bacillus Stearothermophilus ◽  
Cleavage Sites ◽  
Recognition Sequence ◽  
Base Pairs ◽  
Nicking Endonuclease ◽  
Dna Nicking ◽  
Unique Restriction

AbstractN.BstNBI is a unique restriction endonuclease isolated fromBacillus stearothermophilus. We have characterized the recognition sequence and the cleavage site of N.BstNBI. Mapping of cleavage sites of N.BstNBI showed that it recognizes an asymmetric sequence, 5′ GAGTC 3′, and cleaves only on the top strand 4 base pairs away from its recognition sequence. To verify the nicking activity of N.BstNBI, we have constructed two plasmids containing a single recognition sequence (pNB1) or no recognition site (pNB0). When pNB1 and pNB0 were incubated with the enzyme, N.BstNBI nicked only the plasmid pNB1, suggesting that N.BstNBI is a specific nicking endonuclease.

Purification, Properties and Recognition Sequence of Site-specific Restriction Endonuclease from “Acetobacter xylinus”

1983 ◽  
Vol 47 (12) ◽  
pp. 2871-2879
Author(s):  
Hajime Yoshioka ◽  
Hiroshi Nakamura ◽  
Joji Sasaki ◽  
Yasutaka Tahara ◽  
Yuzo Yamada
Keyword(s):  
Restriction Endonuclease ◽  
Recognition Sequence ◽  
Site Specific ◽  
Specific Restriction
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