A supercritical fluid chromatographic method using packed columns for phenylbutazone and oxyphenbutazone in serum, and for phenylbutazone in a dosage form

Background:The concept of green analytical chemistry prevails due to the growing environmental pollution.Objective:Our attempts are to develop simple and eco-friendly method which is non-harmful to the environment by producing minimal waste. In this context, a green liquid chromatographic method was applied for the simultaneous determination of chlorpheniramine maleate, pseudoephedrine hydrochloride and propyphenazone in their combined dosage form.Methods:Separation was carried out using X select HSS RP C18 analytical column (250 × 4.6 mm, 5μm) using methanol - 0.02 M phosphate buffer pH 3 - triethylamine (60:40: 0.1, by volume) as a mobile phase. The separated peaks were detected at 215 nm at a flow rate 1.0 mL/min.Results:Quantification was done over the concentration ranges of 1-25 µg/mL for chlorpheniramine maleate, 5-35 µg/mL for pseudoephedrine hydrochloride and 10-120 µg/mL for propyphenazone. The suggested method was validated with regard to linearity, accuracy and precision according to the International Conference on Harmonization guidelines with good results.Conclusion:It could be used as a safer alternative for routine analysis of the mentioned drugs in quality control laboratories.

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A rapid, sensitive high performance liquid chromatographic method for the determination of meropenem in pharmaceutical dosage form, human serum and urine

Biomedical Chromatography ◽

10.1002/bmc.68 ◽

2001 ◽

Vol 15 (4) ◽

pp. 263-266 ◽

Cited By ~ 28

Author(s):

Yalcin Özkan ◽

İlkay Küçükgüzel ◽

Sibel A. Özkan ◽

Hassan Y. Aboul-Enein

Keyword(s):

Human Serum ◽

High Performance ◽

Dosage Form ◽

Chromatographic Method ◽

High Performance Liquid Chromatographic ◽

Pharmaceutical Dosage Form ◽

Liquid Chromatographic Method ◽

Liquid Chromatographic ◽

Serum And Urine

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A VALIDATED METHOD FOR THE DETERMINATION OF TAPENTADOL HYDROCHLORIDE IN BULK AND ITS PHARMACEUTICAL FORMULATION BY DENSITOMETRIC ANALYSIS

INDIAN DRUGS ◽

10.53879/id.49.12.p0051 ◽

2012 ◽

Vol 49 (12) ◽

pp. 51-55

Author(s):

S Kathirvel ◽

◽

K. Madhu Babu

Keyword(s):

Mobile Phase ◽

High Performance ◽

Dosage Form ◽

Chromatographic Method ◽

Glacial Acetic Acid ◽

Calibration Plot ◽

Pharmaceutical Dosage Form ◽

Aluminum Plates ◽

Tablet Dosage

Described in this manuscript is the first reported new, simple high performance thin layer chromatographic method for the determination of tapentadol hydrochloride in bulk and its tablet dosage form. The drug was separated on aluminum plates precoated with silica gel 60 F254 with butanol: water: glacial acetic acid in the ratio of 6:2:2 (v/v/v) as mobile phase. Quantitative analysis was performed by densitometric scanning at 254 nm. The method was validated for linearity, accuracy, precision and robustness. The calibration plot was linear over the range of 200-600 ng band -1 for tapentadol hydrochloride. The method was successfully applied to the analysis of drug in a pharmaceutical dosage form.

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