Kinetics of protein synthesis in higher organisms in vivo

1976 ◽  
Vol 1 (2) ◽  
pp. 133-136 ◽  
Author(s):  
Audrey E.V. Haschemeyer
2014 ◽  
Vol 10 (10) ◽  
pp. e1003909 ◽  
Author(s):  
Sophia Rudorf ◽  
Michael Thommen ◽  
Marina V. Rodnina ◽  
Reinhard Lipowsky

Parasitology ◽  
1993 ◽  
Vol 107 (3) ◽  
pp. 265-274 ◽  
Author(s):  
R. Harrop ◽  
R. A. Wilson

SummaryThe lung schistosomulum ofSchistosoma mansoniis the target of protective immunity in mice singly vaccinated with irradiated cercariae. Since the effector responses are T cell-mediated, their initiation requires the release of antigens from the intact parasite. We have used the technique of biosynthetic labelling with ‘35S’methionine, before and after transformation of the cercariae, to analyse the kinetics of protein synthesis and release by the schistosomulum. In addition, the proteins present in the soluble fraction of the parasite and those released duringin vitroculture have been characterized. During a 7-day culture period schistosomula derived from labelled cercariae lost proteins most rapidly within the first 3 h after transformation. Two proteins of molecular weight 61 and 20 kDa were dominant and may correspond to areas of proteolytic activity. Analysis of the rate of protein synthesis of schistosomula labelled after transformation revealed four different phases, which may relate to the developmental processes occurringin vivo. During the first 24 h, synthesis was very low, increasing to a plateau and then rising to a peak at day 8; thereafter the rate declined rapidly. Whilst some stage-specific synthesis of proteins was detected in the soluble fractions of the parasite bodies, the pattern of proteins released by cultured larvae was remarkably uniform. At least 15 proteins were detected by autoradiography with bands at 61, 45 and 20 kDa being particularly prominent. These proteins merit further study as potential mediators of the protective immune response.


Science ◽  
1968 ◽  
Vol 160 (3832) ◽  
pp. 1115-1117 ◽  
Author(s):  
R. E. Ecker ◽  
L. D. Smith ◽  
S. Subtelny

2018 ◽  
Vol 46 (22) ◽  
pp. e130-e130 ◽  
Author(s):  
Nadin Haase ◽  
Wolf Holtkamp ◽  
Reinhard Lipowsky ◽  
Marina Rodnina ◽  
Sophia Rudorf

1975 ◽  
Vol 62 (2) ◽  
pp. 433-446
Author(s):  
M. J. Wells ◽  
R. K. O'Dor ◽  
S. K. Buckley

1. Protein synthesis occurs at a high rate in the ovaries of maturing Octopus vulgaris and can be measured from the incorporation of [14C]leucine in vivo and in isolated groups of eggs in vitro. 2. Removal of the optic glands in vivo 1--3 days prior to testing markedly reduces amino acid incorporation in vivo or in vitro. After 5 days in vivo incorporation stops. 3. The rate of incorporation in vitro is increased by the addition of optic gland extract. 4. Analysis of the kinetics of leucine uptake and incorporation in vitro indicates that the hormone has an effect on the inward transport of leucine which is independent of its action on protein synthesis. 5. Electron-microscope studies of the follicle cells and ova show that the former are the site of protein synthesis. 6. Changes in either uptake or incorporation into protein by the follicle cells can be used as a qualitative biolobical assay for the optic gland hormone. Uptake is very easy to measure but incorporation is the more sensitive parameter. Either is potentially suitable as a quantitative assay for this and perhaps also for other molluscan gonadotropins.


1973 ◽  
Vol 51 (10) ◽  
pp. 1428-1432
Author(s):  
R. Kisilevsky ◽  
G. Matheson

In vivo labeling kinetics of (a) nascent peptides on liver polysomes, and (b) proteins of the 100 000 × g supernatant have been determined for ethionine-intoxicated female rats given adenine, to reverse the effect of ethionine. When compared to computer-generated data the results indicate that the reversal effect of adenine is not simply through the increase in the previously reduced rate of initiation. In addition to increasing the rate of initiation, adenine, soon after its administration, reduces the rate of elongation. The rate of elongation subsequently returns to normal levels.


1961 ◽  
Vol 47 (9) ◽  
pp. 1415-1423 ◽  
Author(s):  
R. J. Young ◽  
H. K. Kihara ◽  
H. O. Halvorson

Sign in / Sign up

Export Citation Format

Share Document