THE LEUCOCYTE MIGRATION TECHNIQUE FOR IN VITRO DETECTION OF CELLULAR HYPERSENSITIVITY IN MAN

1971 ◽  
pp. 289-295 ◽  
Author(s):  
Mogens Søborg
Keyword(s):  
Leucocyte Migration ◽  
Migration Technique

scholarly journals Vitamin E isoforms directly bind PKCα and differentially regulate activation of PKCα

2011 ◽  
Vol 441 (1) ◽  
pp. 189-198 ◽  
Author(s):  
Christine A. McCary ◽  
Youngdae Yoon ◽  
Candace Panagabko ◽  
Wonhwa Cho ◽  
Jeffrey Atkinson ◽  
...  
Keyword(s):  
Vitamin E ◽  
Lipid Vesicles ◽  
C2 Domain ◽  
Binding Studies ◽  
Leucocyte Migration ◽  
Protein Kinase Cα ◽  
Oxidative Activation ◽  
Regulatory Effects ◽  
Opposing Effects

Vitamin E isoforms have opposing regulatory effects on leucocyte recruitment during inflammation. Furthermore, in vitro, vitamin E isoforms have opposing effects on leucocyte migration across endothelial cells by regulating VCAM (vascular cell-adhesion molecule)-1 activation of endothelial cell PKCα (protein kinase Cα). However, it is not known whether tocopherols directly regulate cofactor-dependent or oxidative activation of PKCα. We report in the present paper that cofactor-dependent activation of recombinant PKCα was increased by γ-tocopherol and was inhibited by α-tocopherol. Oxidative activation of PKCα was inhibited by α-tocopherol at a 10-fold lower concentration than γ-tocopherol. In binding studies, NBD (7-nitrobenz-2-oxa-1,3-diazole)-tagged α-tocopherol directly bound to full-length PKCα or the PKCα-C1a domain, but not PKCζ. NBD-tagged α-tocopherol binding to PKCα or the PKCα-C1a domain was blocked by diacylglycerol, α-tocopherol, γ-tocopherol and retinol, but not by cholesterol or PS (phosphatidylserine). Tocopherols enhanced PKCα-C2 domain binding to PS-containing lipid vesicles. In contrast, the PKCα-C2 domain did not bind to lipid vesicles containing tocopherol without PS. The PKCα-C1b domain did not bind to vesicles containing tocopherol and PS. In summary, α-tocopherol and γ-tocopherol bind the diacylglycerol-binding site on PKCα-C1a and can enhance PKCα-C2 binding to PS-containing vesicles. Thus the tocopherols can function as agonists or antagonists for differential regulation of PKCα.

Effects of Bothriocephalus acheilognathi on the polarization response of pronephric leucocytes of carp, Cyprinus carpio

2000 ◽  
Vol 74 (3) ◽  
pp. 253-257 ◽  
Author(s):  
P. Nie ◽  
D. Hoole
Keyword(s):  
Cyprinus Carpio ◽  
Infected Fish ◽  
Polarization Response ◽  
Leucocyte Migration ◽  
Significant Difference ◽  
Carp Cyprinus Carpio ◽  
Bothriocephalus Acheilognathi ◽  
Carp Serum

AbstractAn in vitro assay was used to examine the effect of Bothriocephalus acheilognathi Yamaguti, 1934 (Cestoda: Pseudophyllidea) on the polarization response of pronephric leucocytes of carp, Cyprinus carpio. Leucocytes, isolated from naive, naturally-infected fish and carp injected intraperitoneally with cestode extracts, were exposed to parasite extracts (protein concentrations 0–10.0 μg ml-1), for up to 24 h in the presence or absence of carp serum. In general, polarization responses of the pronephric leucocytes, primarily neutrophils and eosinophils, increased with incubation time although there was no significant difference in the response induced by the different protein concentrations. Differences in the polarization response were, however, observed in naive, naturally infected and injected fish and the cells responded differently in the presence and absence of carp serum. In the absence of carp serum the polarization response of pronephric leucocytes in vitro was significantly reduced with cells obtained from injected and naturally infected fish compared with those obtained from naive carp. This suppression of leucocyte migration was however reduced by the addition of carp serum to the in vitro system. The role of this interaction between the possible suppression of polarization induced by the parasite and stimulation by serum is discussed.

scholarly journals THE IN VITRO ACTION OF HYDROCORTISONE ON LEUCOCYTE MIGRATION

1958 ◽  
Vol 107 (2) ◽  
pp. 211-218 ◽  
Author(s):  
Melvin M. Ketchel ◽  
Cutting B. Favour ◽  
Somers H. Sturgis ◽  
Keyword(s):  
Dose Response ◽  
Response Curve ◽  
Dose Response Curve ◽  
Logarithmic Plot ◽  
Leucocyte Migration

Hydrocortisone inhibits the ameboid migration of human leucocytes when added in vitro. The dose-response curve for the reaction between this steroid and leucocytes can be best expressed by a logarithmic plot of the steroid concentrations. Tetrahydrocortisone and desoxycorticosterone had no effect on in vitro leucotyte migration.

scholarly journals Novel Pharmacological Properties of Dinoponera quadriceps Giant Ant Venom

2015 ◽  
Vol 10 (9) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Juliana da Costa Madeira ◽  
Yves Patric Quinet ◽  
Dayanne Terra Tenório Nonato ◽  
Paloma Leão Sousa ◽  
Edna Maria Camelo Chaves ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Anticoagulant Activity ◽  
Adenosine Diphosphate ◽  
South American ◽  
Crude Venom ◽  
Leucocyte Migration ◽  
Ant Venom ◽  
Hymenopteran Species ◽  
Anti Inflammatory

The South American giant ant, Dinoponera quadriceps (Hymenoptera, Formicidae, Ponerinae), produces proteinaceous venom that has antinociceptive, neuroprotective and antimicrobial effects, thereby supporting the popular use of these ants to treat asthma, rheumatism, earache and back pain. Anticoagulant activity is another biological property that has been shown for the venom of other hymenopteran species, like wasps. The aim of this study was to assess the anti-inflammatory, anticoagulant and antiplatelet properties of D. quadriceps venom (DqV). DqV anti-inflammatory activity was assessed by intravenous administration in Swiss mice in the models of paw edema and peritonitis. In vitro, DqV was assessed in coagulation (activated partial thromboplastin time) and platelet aggregation tests. DqV inhibited (27–33%) the edema elicited by carrageenan and the leucocyte migration (43%) elicited by zymosan. DqV decreased by 57% and 42%, respectively, the content of malondialdehyde and nitrite in the peritoneal fluid. DqV prolonged (1.8x) the clotting time and decreased (27%) the platelet aggregation induced by adenosine diphosphate. The crude venom of D. quadriceps presents an anti-inflammatory effect in mice and in vitro anticoagulant and antiplatelet effects.

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