A Chronology of the Development of Aqueous Two-Phase Systems as a Viable Liquid-Liquid Extraction for Biological Products

2019 ◽  
Author(s):  
Diogo F. Faria ◽  
Tiago P. Silva ◽  
M. Raquel Aires-Barros ◽  
Ana M. Azevedo
Keyword(s):  
Liquid Extraction ◽  
Two Phase ◽  
Biological Products ◽  
Liquid Liquid Extraction ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Fractionation of aqueous sodium salts by liquid–liquid extraction in aqueous two phase systems

2014 ◽  
Vol 125 ◽  
pp. 208-215 ◽  
Author(s):  
Miran Milosevic ◽  
Koen J.J. Staal ◽  
Gerrald Bargeman ◽  
Boelo Schuur ◽  
André B. de Haan
Keyword(s):  
Liquid Extraction ◽  
Two Phase ◽  
Sodium Salts ◽  
Aqueous Sodium ◽  
Liquid Liquid Extraction ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

scholarly journals A stable liquid–liquid extraction system for clavulanic acid using polymer-based aqueous two-phase systems

2012 ◽  
Vol 98 ◽  
pp. 441-450 ◽  
Author(s):  
Jorge F.B. Pereira ◽  
Valéria C. Santos ◽  
Hans-Olof Johansson ◽  
José A.C. Teixeira ◽  
Adalberto Pessoa
Keyword(s):  
Clavulanic Acid ◽  
Liquid Extraction ◽  
Extraction System ◽  
Two Phase ◽  
Liquid Liquid Extraction ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

scholarly journals Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems

2003 ◽  
Vol 46 (4) ◽  
pp. 741-750 ◽  
Author(s):  
Xu Yan ◽  
Maria Aparecida Souza ◽  
Marcela Z. R. Pontes ◽  
Michele Vitolo ◽  
Adalberto Pessoa Júnior
Keyword(s):  
Reactive Dyes ◽  
Liquid Extraction ◽  
Phase System ◽  
Two Phase ◽  
Affinity Ligands ◽  
Aqueous Two Phase System ◽  
Liquid Liquid Extraction ◽  
Commercial Scale ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

From analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aqueous two-phase systems in regard to the different ligands, including reactive dyes, metal ions and other ligands. Some integration of aqueous two-phase system with other techniques for more effective purification of enzymes are also presented.

scholarly journals Liquid-liquid extraction of protease from cold-adapted yeastRhodotorula mucilaginosaL7 using biocompatible and biodegradable aqueous two-phase systems

2015 ◽  
Vol 51 (1) ◽  
pp. 57-67 ◽  
Author(s):  
Luciana Daniela Lario ◽  
Luciana Pellegrini Malpiedi ◽  
Jorge Fernando Brandão Pereira ◽  
Lara Durães Sette ◽  
Adalberto Pessoa-Junior
Keyword(s):  
Liquid Extraction ◽  
Two Phase ◽  
Cold Adapted ◽  
Liquid Liquid Extraction ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

scholarly journals Liquid–liquid extraction of lipase produced by psychrotrophic yeast Leucosporidium scottii L117 using aqueous two-phase systems

2015 ◽  
Vol 156 ◽  
pp. 215-225 ◽  
Author(s):  
Alysson Wagner Fernandes Duarte ◽  
André Moreni Lopes ◽  
João Vitor Dutra Molino ◽  
Adalberto Pessoa ◽  
Lara Durães Sette
Keyword(s):  
Liquid Extraction ◽  
Two Phase ◽  
Liquid Liquid Extraction ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

scholarly journals Separation of digoxin by luiquid-luiquid extraction from extracts of foxglove secondary glycosides

2014 ◽  
Vol 68 (2) ◽  
pp. 161-170 ◽  
Author(s):  
Vesna Novkovic ◽  
Ljiljana Stanojevic ◽  
Milorad Cakic ◽  
Vlada Veljkovic ◽  
Mihajlo Stankovic
Keyword(s):  
Aqueous Ethanol ◽  
Volume Ratio ◽  
Liquid Extraction ◽  
Light Phase ◽  
Initial Amount ◽  
Two Phase ◽  
Liquid Liquid Extraction ◽  
Extraction Degree ◽  
Phase Systems ◽  
Extracting Solvent

The present study deals with the extraction of digoxin (Dgx) from chloroform and trichloroethylene extracts of the secondary glycosides of fermented foxglove (Digitalis lanata Ehrh.) foliage by liquid-liquid extraction. The extraction degree (ED) of Dgx achieved by maceration and percolation using 10% vol. aqueous ethanol solutions were higher than 95%. Using trichlorethylene and chloroform, the ED of Dgx of about 100% and 96%, respectively from the liquid ethanolic extracts (macerate or percolate) were achieved by the four-cycle extraction. Fifteen separating funnels were employed for the liquid-liquid extraction. Three different four-component two-phase systems (ethanol:water - chloroform:ethyl acetate, ethanol:water - chloroform:trichloroethylene and ethanol:water - trichloroethylene:ethyl acetate) were tested as an extracting solvent to get the final product having more than 98% of Dgx. The initial amount of the chloroform or trichloroethylene extract in the light phase was varied between 5 and 25 g/L, while the volume ratio of light and heavy phases was in the range of 1:1 to 1:2. The best Dgx yield of 98% was achieved with the system ethanol:water - chloroform:trichloroethylene 35:15:20:30 at the volume ratio of the phases of 1:1.1 and at the initial amount of the extract of 15 g/L. Purity of the separated digoxin was 99.8 %.

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