Effects of Trichoderma asperellum BV10 and Bacillus amyloliquefaciens BV03 in Meloidogyne incognita Control Considering Three Different Management Systems

Crop yield decrease is the main concern when a pathogen or plague is identified in an agriculture field. Thus, part of this issue can be attributed to plant-parasitic nematodes (PPNs), such as Meloidogyne species, due to, most of the time, the hard diagnosis, and non-specific symptoms. Its management is mainly based on chemical pesticides, followed by a few potential biological control agents, and the management system. Therefore, this study aimed to evaluate the effects of biological agents in Meloidogyne incognita control in different soil systems. For that, two biological products were chosen, Trichoderma asperellum BV10 and Bacillus amyloliquefaciens BV03, and soils were sampled from three different managements systems: (i) soybean no-tilled system at Goiás state, Brazil; (ii) forest soil at Goiás state, Brazil, and (iii) soybean conventional managed system at Mato Grosso do Sul state, Brazil. Biocontrol and growth promotion effects, volatile organic compounds (VOCs) and soil respiration were determined in vegetation house and laboratory, respectively. As a result, both BV10 and BV03 had significant nematode control activity, comparing to control treatment, in all the three soils systems. Plus, the number of immobile nematodes by potential VOCs had significant increase when BV03 was applied, while the application of BV10 agent raised the soil respiration rate. In conclusion, both biocontrol agents presented great efficiency in control M. incognita, with better performance of BV03. Lastly, more studies must be done to elucidate how the resident soil microbiome can influence on biocontrol agent establishment and performance, as well as the consequence of the application of biological products on soil microbiome network.

Influence of biopreparations on formation of elements of productivity of spring barley

Relevance. The strategy for the development of agriculture in Russia and its biologization determine the relevance of our research. Empirical studies are aimed at studying the effect of biological preparations on the formation of elements of the structure of the yield of spring barley. The data presented in the work continues the research carried out in 2016–2020 in the Rostov region.Methods. Studied biopreparations are: Biodux; Trainer; Artafite; Obereg; Fulvogumate. Control — water treatment. Spraying was carried out according to the recommendations for the use of preparations: 1st — in the tillering phase, 2nd — in the heading phase. The total area under the experiments is 600 m2, the area of each variant is 25 m2, the replication is 4 times. The link in the crop rotation is “sunflower — spring barley”. The soils of the experimental plot are ordinary chernozem. Used variety is Leon.Results. In 2021, full sprouting of barley was observed on the 8th day after sowing. This is due to sufficient warming up of the sowing layer of soil (+8 °C) and moisture 28 mm (in a layer of 0–10 cm). Studies have shown that field germination of barley was 88%. The use of biological products influenced the survival rate of plants to harvest which wasmore than 80%. In the variants with the use of Biodux and Obereg, this figure was 86 and 87%, respectively. Biopreparations stimulated the growth of productive stems, productive bushiness increased in comparison with the control (1,21) to 1,28–1,33. The mass of a thousand grains in the variants exceeded the control, especially with the use of Obereg — 45,6 g, which is 2,9 g more than the control. The number of grains in an ear ranged from 17,7 to 18 pieces, which is on average 1 piece more than in the control. The highest productivity was formed by barley plants under the influence of the biopreparations Obereg and Biodux. Thus, the increase in productivity under the influence of biological products due to all elements of the productivity of barley plants has been reliably proven.

Effectiveness of biotechnology on the basis of associative microorganisms at the early stages of forestry process of the Pinus sylvestris L.

The transition to biologically based technologies is one of the priority scientific areas of agriculture and forestry in Russia. The aim of the work is to develop and modify the technology of using biological products on forest tree species and to test it on Scots pine. The effect of pre-sowing inoculation of pine seeds with biological products on soil germination, safety of one- and two-year-old seedlings and their biometric characteristics was studied. The results of the analysis of soil germination of seeds and the safety of 1-year-old seedlings showed that these biological products can be introduced into the technology of growing planting material of Scots pine. It was revealed that the seeds of sensitive trees of the Stupinskaya population turned out to be the most responsive to inoculation with biological products. A stimulating effect has been established on the survival rate of seedlings, preservation, height, growth and diameter of 2-year-old plants in a forestry area (Vernadsky forestry, Tambov region). The height of 2-year-old seedlings of pine 'Ostrogozhskaya' exceeds the control by an average of 18.1%. The use of this biotechnology in forestry production will create favorable conditions for the germination of pine seeds, the growth of seedlings and their higher survival rate.

FROM INDUSTRY TO TOURISM: THE CASE OF LX FACTORY IN THE ALCÂNTARA DISTRICT (LISBON)

In the past few years Lisbon has been through many changes, most of them related to the tourism growth. Alcântara is one of the neighbourhoods that became popular, once an industrial area that is presently experiencing a process of urban regeneration. Alcântara is probably the best district of Lisbon to recall the industrial era. At the same time, it has all the services that it needs to welcome visitors: diversity of tourist supplies; a vibrant image, trendy, but also authentic; a barycentric position between two well-known quarters (Baixa and Belém); a good accessibility, still being strengthened; a fast growth in accommodation supply. Arguably, an important role in the tourist development of Alcântara is played by the LX Factory, a former industrial area of around 23.000 m2, where important companies were located. LX Factory is considered a successful experiment of reconversion of an industrial space into a multifunctional complex, which still preserves the former factory atmosphere, although according to a contemporary formula. The LX Factory was inaugurated in 2008. It hosts design offices, art ateliers, start-ups, shops, restaurants, cafés, night clubs, co-working spaces, and even a hostel. It also hosts cultural activities, concerts, workshops, and a Sunday market of vintage and biological products. A previous research (Zarrilli et al., 2019) showed that LX Factory is by far the main tourist attractor of Alcântara. In this contribution, we will try to deeper investigate what we may call the LX Factory phenomenon, in terms of tourist image, flows, motivations and assessments. At this aim, a questionnaire was administered to a sample of shop owners located in its facilities. Conclusions allow us to understand LX Factory main issues from the shop owners’ perspective, regarding the commercial activity, the current image, the people who visit it, and their opinion about the neighbourhood.

Produtos naturais e os riscos à mucosa oral e face: um protocolo de revisão de escopo

O objetivo deste protocolo é garantir a transparência do processo da revisão de escopo, pré-definindo os objetivos, os métodos, os critérios de elegibilidade das fontes de evidências e os dados relevantes a respeito das reações adversas em mucosa oral e face provocadas pelo uso dos produtos naturais. Este protocolo foi registrado na Open Science Framework e seguiu as recomendações do Manual do Instituto Joanna Briggs. A estratégia de busca será estruturada para o PubMed e posteriormente adaptada para as outras bases de dados, como Scopus, Web of Science, Embase, LILACS e LIVIVO, além da literatura cinzenta (Google Scholar e OpenGrey), utilizando os seguintes termos e suas palavras relacionadas: “Biological Products”, “Natural Products”, “Phytotherapy”, “Chemical Burn”, “Adverse Effect”, “Oral mucosa” e “Face”. Adicionalmente, será feita a busca nas listas de referências dos artigos incluídos. Relatos de caso que apresentem reações adversas à mucosa oral e à face, sem distinção de sexo, idade ou etnia dos pacientes serão incluídos na revisão. Estudos clínicos, in vitro, em modelos animais, revisões de literatura e artigos de opinião serão excluídos. A seleção dos estudos será conduzida em duas fases por dois examinadores independentes e participação do terceiro revisor em caso de discordância. Os dados também serão extraídos de maneira independente, com o uso de formulário desenvolvido pelos próprios autores, e apresentados em tabela e/ou figuras e de forma narrativa. Este protocolo auxiliará no desenvolvimento da revisão de escopo, para mapeamento e sumarização das evidências.

Virome Characterization in Commercial Bovine Serum Batches—A Potentially Needed Testing Strategy for Biological Products

Bovine serum has been widely used as a universal supplement in culture media and other applications, including the manufacture of biological products and the production of synthetic meat. Currently, commercial bovine serum is tested for possible viral contaminants following regional guidelines. Regulatory agencies’ established tests focused on detecting selected animal origin viruses and are based on virus isolation, immunofluorescence, and hemadsorption assays. However, these tests may fail to detect new or emerging viruses in biological products. High-throughput sequencing is a powerful option since no prior knowledge of the viral targets is required. In the present study, we evaluate the virome of seven commercial batches of bovine serum from Mexico (one batch), New Zealand (two batches), and the United States (four batches) using a specific preparation and enrichment method for pooled samples and sequencing using an Illumina platform. A variety of circular replicase-encoding single-stranded (CRESS) DNA families (Genomoviridae, Circoviridae, and Smacoviridae) was identified. Additionally, CrAssphage, a recently discovered group of bacteriophage correlated with fecal contamination, was identified in 85% of the tested batches. Furthermore, sequences representing viral families with single-stranded DNA (Parvoviridae), double-stranded DNA (Polyomaviridae and Adenoviridae), single-stranded RNA (Flaviviridae, Picornaviridae, and Retroviridae), and double-stranded RNA (Reoviridae) were identified. These results support that high-throughput sequencing associated with viral enrichment is a robust tool and should be considered an additional layer of safety when testing pooled biologicals to detect viral contaminants overlooked by the current testing protocols.

Effectiveness of biological protection of cabbage against pests in the conditions of the Primorsky Territory

The results of the use of entomophages and biological products to regulate the number of cabbage pests (cabbage moth Mamestra brassicae L., cabbage butterfly Pieris brassicae L., turnip butterfly Pieris rapae L., diamondback moth Plutella xylostella L.) are presented. The study was carried out in the Primorsky Territory in 2018-2020. The efficiency of Trichogramma ussuricum Sorokina applications was assessed on cabbage varieties. The effectiveness of the entomophage against the cabbage moth varied from 33.3 to 66.6%, against the turnip butterfly - from 32.6 to 70.2%. In field experiments the effectiveness of biological products Fitoverm EC (0.09 l/ha), Akarin EC (1.6 l/ha), Proclaim WG (0.3 kg/ha), Bitoxibacillin (10 l/ha), Bitoxibacillin P (2 kg/ha), Lepidocid SC (2 l/ha), Lepidocid P (2 kg/ha) against diamondback moth are studied. Cabbage plants were sprayed with the preparations once. Pest counts were carried out before treatment and after treatment on the 5th, 10th and 15th day in accordance with the approved methods. Bioinsecticide Proclaim showed a high efficiency of 93.0-100% on the 5-10th day. The effectiveness of preparations based on aversectin C and avertin N was 65.0-88.6%. Using the biological product Bitoxibacillin and Lepidocid a decrease the number of diamondback moth relative to the control by 61.2-97.5 и 65.0-78.0% was registered.

Influence of biological products on the parameters of the root system of wood cuttings of black currant (Ribes nigrum L.)

Black currant (Ribes nigrum L.) is one of the most widespread berry crops in Russia. The need for planting material of black currant is increasing every year, because the area for laying industrial plantations is increasing. The purpose of our research is to assess the effect of combinations of biological products on the rooting rate of cuttings and on the parameters of the root system of black currant. The objects of research are black currant varieties - Dar Smolyaninova, Sevchanka, Yadrenaya. In the research, there are two combinations of biological products: based on Trichoderma viride + water and the combined use Trichoderma viride and Pseudomonas fluorescens. The experiment was carried out in 2021 in the Moscow region. We found that there is no reliable effect of the products on the increase of the root system volume and the roots length. The coefficients of variation for the studied varieties of the root system volume and along the length are very high, that does not make the planting material leveled according to these indicators. Keywords: BLACK CURRANT, RIBES NIGRUM, CUTTINGS, REPRODUCTION, ROOTING, BIOLOGICAL PRODUCTS, TRICHODERMA VIRIDE, PSEUDOMONAS FLUORESCENS