Identification and Isolation of Human Splenic Macrophages, Lymphocytes, and Related Cells with in Situ Immunohistochemical Techniques and Countercurrent Centrifugal Elutriation

1987 ◽  
pp. 309-329
Author(s):  
PATRICK J. BUCKLEY
Keyword(s):  
Centrifugal Elutriation ◽  
Splenic Macrophages ◽  
Immunohistochemical Techniques

Isolation of human splenic macrophages and lymphocytes by countercurrent centrifugal elutriation

1984 ◽  
Vol 66 (2) ◽  
pp. 201-217 ◽  
Author(s):  
Patrick J. Buckley ◽  
Robert H.J. Beelen ◽  
Jacquelyn Burns ◽  
Carolyn M. Beard ◽  
Susan A. Dickson ◽  
...  
Keyword(s):  
Centrifugal Elutriation ◽  
Splenic Macrophages

scholarly journals Retention of dopamine 2 receptor mRNA and absence of the protein in craniospinal and extracranial metastasis of a malignant prolactinoma: a case report

2002 ◽  
pp. 81-88 ◽  
Author(s):  
J Winkelmann ◽  
U Pagotto ◽  
M Theodoropoulou ◽  
K Tatsch ◽  
W Saeger ◽  
...  
Keyword(s):  
Single Case ◽  
Clinical History ◽  
Extracranial Metastasis ◽  
Invasion And Metastasis ◽  
History Of ◽  
Dopamine 2 Receptor ◽  
Immunohistochemical Techniques

OBJECTIVES: The case presented here describes the clinical evolution of a malignant prolactinoma with occurrence of intra- and extra-cranial metastases. In this case, the presence of dopamine 2 receptor (D2R) was studied at the mRNA and protein level, in order to understand the pathological background of the resistance to treatment with different dopamine agonists. DESIGN: Together with an extensive description of the clinical history of this case, a combination of in vitro and in vivo techniques was performed to provide the basis of the dopamine resistance developed in the course of the disease. METHOD: A comparison of the D2R was performed in specimens obtained at presentation of the disease compared with autoptic specimens derived from local invasion and metastasis using in situ hybridization and immunohistochemical techniques. RESULTS: Intact D2R mRNA was found in the primitive tumor and metastatic tissues, whereas protein for the same receptor was present only in the tissues derived from neurosurgical operations and not in the metastases obtained post-mortem. CONCLUSION: This is the first report of the absence of D2R protein despite the retention of the transcript in an advanced stage of a malignant prolactinoma. The findings of this single case suggest the hypothesis that postranscriptional mechanisms may contribute to the development of dopamine resistance in prolactinomas.

Cytochemical features common to nucleoli and cytoplasmic nucleoloids of Olea europaea meiocytes: detection of rRNA by in situ hybridization

1994 ◽  
Vol 107 (2) ◽  
pp. 621-629 ◽  
Author(s):  
J.D. Alche ◽  
M.C. Fernandez ◽  
M.I. Rodriguez-Garcia
Keyword(s):  
In Situ Hybridization ◽  
Olea Europaea ◽  
Electron Microscopic ◽  
Phosphorylated Proteins ◽  
Immunocytochemical Techniques ◽  
Fibrillar Component ◽  
Immunohistochemical Techniques ◽  
Microscopic Techniques ◽  
Olea Europaéa

We used light and electron microscopic techniques to study the composition of cytoplasmic nucleoloids during meiotic division in Olea europaea. Nucleoloids were found in two clearly distinguishable morphological varieties: one similar in morphology to the nucleolus, and composed mainly of dense fibrillar component, and another surrounded by many ribosome-like particles. Cytochemical and immunocytochemical techniques showed similar reactivities in nucleoloids and the nucleolus: both are ribonucleoproteic in nature, and possess argyrophillic, argentaffinic and highly phosphorylated proteins. Immunohistochemical techniques failed to detect DNA in either structure. In situ hybridization to a 18 S rRNA probe demonstrated the presence of ribosomal transcripts in both the nucleolus and nucleoloids. These similarities in morphology and composition may reflect similar functionalities.

Collagenase-3 (MMP-13) Expression in Cutaneous Malignant Melanoma

2005 ◽  
Vol 20 (4) ◽  
pp. 242-248 ◽  
Author(s):  
M.D. Corte ◽  
L.O. Gonzalez ◽  
M.G. Corte ◽  
I. Quintela ◽  
I. Pidal ◽  
...  
Keyword(s):  
Malignant Melanoma ◽  
Cutaneous Malignant Melanoma ◽  
Matrix Metalloproteases ◽  
Benign Lesions ◽  
Melanocytic Nevi ◽  
Important Barrier ◽  
Positive Immunostaining ◽  
Immunohistochemical Techniques

Background Matrix metalloproteases (MMPs), enzymes with the ability to degrade the extracellular matrix, play an important role in tissue invasion by cutaneous malignant melanoma (CMM). One specific MMP, collagenase-3 (MMP-13), is thought to have a key function in the activation of MMP. Aims To evaluate the expression of MMP-13 in CMM and assess its possible relationship to clinical and pathological parameters. Methods MMP-13 expression was analyzed in 51 paraffin-embedded tumor samples from patients with invasive CMM, ten samples from in situ melanomas, and in eight samples from benign lesions (three dermal melanocytic nevi, three compound melanocytic nevi and two atypical melanocytic nevi) using immunohistochemical techniques. The median follow-up period in patients with invasive CMM was 50 months. Results Benign lesions were consistently negative for MMP-13, whereas three of the ten in situ melanomas (30%) and 23 of the 51 invasive CMMs (45%) showed positive immunostaining for MMP-13. The percentage of MMP-13-positive tumors correlated significantly and positively with the mitotic index (p=0.002) in invasive CMM. However, our results did not show any significant association between tumoral MMP-13 expression and relapse-free survival in patients with invasive CMM. Conclusions MMP-13 appears to be a factor associated with tumor aggressiveness in CMM. It seems to eliminate an important barrier not only against tumoral invasion but also against proliferation.

scholarly journals Encephalopathy in Liver Transplantation: Neuropathology and CMV Infection

1990 ◽  
Vol 17 (4) ◽  
pp. 378-381 ◽  
Author(s):  
C. Power ◽  
S.D. Poland ◽  
K.H. Kassim ◽  
J.C.E. Kaufmann ◽  
G.P.A. Rice
Keyword(s):  
Liver Transplantation ◽  
Brain Infarction ◽  
Central Pontine Myelinolysis ◽  
B Cell Lymphoma ◽  
Neurological Dysfunction ◽  
Pontine Myelinolysis ◽  
Immunohistochemical Techniques ◽  
Central Pontine

ABSTRACT:The clinical histories and pathological findings of 27 autopsied cases of orthotopic liver transplantation (OLT) were reviewed. Fatal OLT was complicated in 93% of cases by neurological dysfunction, usually manifested by encephalopathy, with or without seizures. The etiology of the encephalopathy was largely multifactorial (44%) or undetermined (20%). Subarachnoid hemorrhage, central pontine myelinolysis, meningitis, brain infarction, polyclonal B cell lymphoma and spinal cord necrosis were common neuropathological findings. These diagnoses were often masked by other systemic illnesses. The role of cytomegalovirus (CMV) in neurologic dysfunction was explored with in situ hybridization and immunohistochemical techniques. OLT cases showed a significantly higher (89%) frequency of CMV genomic material in brain tissue compared to age-matched non immunocompromised (NIC) patients (23%). All OLT cases with encephalopathy of undetermined cause demonstrated unusually prominent hybridization to the CMV probe. CMV may be an important cause of encephalopathy in such patients.

Rendez vous with Saturn's rings

1984 ◽  
Vol 75 ◽  
pp. 743-759 ◽  
Author(s):  
Kerry T. Nock
Keyword(s):  
Solar Energy ◽  
Electric Propulsion ◽  
Power Source ◽  
Propulsion System ◽  
Low Thrust ◽  
Ring Plane ◽  
The Earth ◽  
Total Impulse ◽  
Saturn's Rings

ABSTRACTA mission to rendezvous with the rings of Saturn is studied with regard to science rationale and instrumentation and engineering feasibility and design. Future detailedin situexploration of the rings of Saturn will require spacecraft systems with enormous propulsive capability. NASA is currently studying the critical technologies for just such a system, called Nuclear Electric Propulsion (NEP). Electric propulsion is the only technology which can effectively provide the required total impulse for this demanding mission. Furthermore, the power source must be nuclear because the solar energy reaching Saturn is only 1% of that at the Earth. An important aspect of this mission is the ability of the low thrust propulsion system to continuously boost the spacecraft above the ring plane as it spirals in toward Saturn, thus enabling scientific measurements of ring particles from only a few kilometers.

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