Antineutrophil Cytoplasmic Autoantibodies with Specificity for Myeloperoxidase

2014 ◽  
pp. 105-113
Author(s):  
Cees G.M. Kallenberg
Keyword(s):  
Antineutrophil Cytoplasmic Autoantibodies

Potential Interference by Antineutrophil Cytoplasmic Autoantibodies in Myeloperoxidase Immunoassays

2008 ◽  
Vol 54 (12) ◽  
pp. 2084-2086 ◽  
Author(s):  
Saul A Datwyler ◽  
Stephen C Hsu ◽  
Matthew S Matias ◽  
David P Pacenti ◽  
Jessie Shih
Keyword(s):  
Antineutrophil Cytoplasmic Autoantibodies

Antineutrophil cytoplasmic autoantibodies in patients with systemic sclerosis

1996 ◽  
Vol 134 (3) ◽  
pp. 407-410 ◽  
Author(s):  
S. AKIMOTO ◽  
O. ISHIKAWA ◽  
T. TAMURA ◽  
Y. MIYACHI
Keyword(s):  
Systemic Sclerosis ◽  
Antineutrophil Cytoplasmic Autoantibodies

Wegener's Granulomatosis and Antineutrophil Cytoplasmic Autoantibodies

1993 ◽  
Vol 102 (11) ◽  
pp. 906-908 ◽  
Author(s):  
John G. Batsakis ◽  
Adel K. El-Naggar
Keyword(s):  
Laboratory Test ◽  
Wegener’S Granulomatosis ◽  
Wegener's Granulomatosis ◽  
Proteinase 3 ◽  
Inflammatory Disorders ◽  
Serologic Tests ◽  
Criteria For Diagnosis ◽  
Antineutrophil Cytoplasmic Autoantibodies

Serologic tests for antineutrophil cytoplasmic autoantibodies can serve as markers for a number of necrotizing vasculitides and other inflammatory disorders. In the case of Wegener's granulomatosis, an immunofluorescent cytoplasmic pattern and immunoassay for proteinase 3 are quite specific for the disease and are capable of serving as additional criteria for diagnosis. As with any laboratory test, however, results are to be interpreted in the whole clinical and pathologic framework of the disease.

scholarly journals Antineutrophil Cytoplasmic Autoantibodies Interact with Primary Granule Constituents on the Surface of Apoptotic Neutrophils in the Absence of Neutrophil Priming

1996 ◽  
Vol 184 (6) ◽  
pp. 2231-2242 ◽  
Author(s):  
Hannah M. Gilligan ◽  
Brunel Bredy ◽  
Hugh R. Brady ◽  
Marie-Josée Hébert ◽  
Henry S. Slayter ◽  
...  
Keyword(s):  
Cell Surface ◽  
Intact Cell ◽  
Immunogold Labeling ◽  
Pathogenic Role ◽  
Anca Associated Vasculitis ◽  
Antineutrophil Cytoplasmic Autoantibodies ◽  
Neutrophil Priming ◽  
Gain Access ◽  
Primary Granule

The pathogenic role of antineutrophil cytoplasmic autoantibodies (ANCA) remains controversial because of the difficulty in explaining how extracellular ANCA can interact with intracellular primary granule constituents. It has been postulated that cytokine priming of neutrophils (PMN), as may occur during a prodromal infection, is an important trigger for mobilization of granules to the cell surface, where they may interact with ANCA. We show by electron microscopy that apoptosis of unprimed PMN is also associated with the translocation of cytoplasmic granules to the cell surface and alignment just beneath an intact cell membrane. Immunofluorescent microscopy and FACS® analysis demonstrate reactivity of ANCA-positive sera and antimyeloperoxidase antibodies with apoptotic PMN, but not with viable PMN. Moreover, we show that apoptotic PMN may be divided into two subsets, based on the presence or absence of granular translocation, and that surface immunogold labeling of myeloperoxidase occurs only in the subset of PMN showing translocation. These results provide a novel mechanism that is independent of priming, by which ANCA may gain access to PMN granule components during ANCA-associated vasculitis.

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