INFLUENCE OF MEDIUM COMPOSITION ON THE DEATH AND LYSIS OF HYBRIDOMA CELLS IN CONTINUOUS CULTURES

1992 ◽  
pp. 122-124 ◽  
Author(s):  
J.L. GOERGEN ◽  
A. MARC ◽  
J.M. ENGASSER
Author(s):  
Jean-Marc Engasser ◽  
Annie Marc ◽  
Marc Cherlet ◽  
Pierre Nabet ◽  
Patricia Franck

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4171
Author(s):  
Jenny Fabiola López-Hernández ◽  
Pedro García-Alamilla ◽  
Diana Palma-Ramírez ◽  
Carlos Alfonso Álvarez-González ◽  
Juan Carlos Paredes-Rojas ◽  
...  

Microalgae, including cyanobacteria, represent a valuable source of natural compounds that have remarkable bioactive properties. Each microalga species produces a mixture of antioxidants with different amounts of each compound. Three aspects are important in the production of bioactive compounds: the microalga species, the medium composition including light supplied and the photobioreactor design, and operation characteristics. In this study, the antioxidant content and productivity performance of four microalgae were assessed in batch and continuous cultures. Biomass productivity by the four microalgae was substantially enhanced under continuous cultivation by 5.9 to 6.3 times in comparison with batch cultures. The energetic yield, under the experimental conditions studied, ranged from 0.03 to 0.041 g biomass kJ−1. Phenols, terpenoids, and alkaloids were produced by Spirulinaplatensis, Isochrysisgalbana, and Tetraselmissuecica, whereas tocopherols and carotenoids were produced by the four microalgae, except for phycocyanin and allophycocyanin, which were only produced by S. platensis and Porphyridiumcruentum. The findings demonstrate that the continuous cultivation of microalgae in photobioreactors is a convenient method of efficiently producing antioxidants.


Author(s):  
Jean-Marc Engasser ◽  
Annie Marc ◽  
Marc Cherlet ◽  
Pierre Nabet ◽  
Patricia Franck

1990 ◽  
Vol 6 (2) ◽  
pp. 114-120 ◽  
Author(s):  
Jonathan F. Petersen ◽  
Larry V. McIntire ◽  
Eleftherios Terry Papoutsakis

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.


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