High-throughput polymerase chain reaction chemiluminescent enzyme immunoassay for typing and quantifying human papillomavirus DNAs

2004 ◽  
Vol 332 (2) ◽  
pp. 349-357 ◽  
Author(s):  
Simone Ambretti ◽  
Mara Mirasoli ◽  
Simona Venturoli ◽  
Marialuisa Zerbini ◽  
Mario Baraldini ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papillomavirus ◽  
Enzyme Immunoassay ◽  
High Throughput ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Chemiluminescent Enzyme Immunoassay

scholarly journals Evaluation of A Chemiluminescent Enzyme Immunoassay-Based High-Throughput SARS-CoV-2 Antigen Assay For The Diagnosis of COVID-19: The VITROS® SARS-CoV-2 Antigen Test

2021 ◽  
Author(s):  
Nanako Matsuzaki ◽  
Yuta Orihara ◽  
Masahiro Kodana ◽  
Yutaro Kitagawa ◽  
Masaru Matsuoka ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
High Throughput ◽  
Nasopharyngeal Swab ◽  
Antigen Test ◽  
Chain Reaction ◽  
Viral Loads ◽  
Antigen Assay ◽  
Polymerase Chain ◽  
Dose Dependent ◽  
Chemiluminescent Enzyme Immunoassay

Abstract A high-throughput, fully automated antigen detection test for SARS-CoV-2 is a viable alternative to reverse transcription polymerase chain reaction (RT-qPCR) for mass screening during outbreaks. In this study, we compared RT-qPCR for viral load and the VITROS® SARS-CoV-2 Antigen Test with reference to the results of the LUMIPULSE® SARS-CoV-2 Ag Test. Of 128 nasopharyngeal swab specimens taken from patients suspected of being infected with SARS-CoV-2, 49 were positive and 79 were negative according to RT-qPCR. Consistent dose-dependent detection with VITROS® assay was successfully achieved when using nasopharyngeal swab specimens with Ct values of ≤32.0, whereas the CLEIA-based the LUMIPULSE® assay was able to detect lower viral loads compared with the VITROS® assay. Our results show that the performance of the VITROS® assay was satisfactory for the diagnosis of contagious COVID-19 patients in the clinical setting.

scholarly journals The Use of General Primers in the Polymerase Chain Reaction Permits the Detection of a Broad Spectrum of Human Papillomavirus Genotypes

1990 ◽  
Vol 71 (1) ◽  
pp. 173-181 ◽  
Author(s):  
P. J. F. Snijders ◽  
A. J. C. van den Brule ◽  
H. F. J. Schrijnemakers ◽  
G. Snow ◽  
C. J. L. M. Meijer ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papillomavirus ◽  
Broad Spectrum ◽  
Chain Reaction ◽  
Polymerase Chain
Sign in / Sign up

Export Citation Format

Share Document