Single nucleotide polymorphisms of thymidine kinase and DNA polymerase genes in clinical herpes simplex virus type 1 isolates associated with different resistance phenotypes

2014 ◽  
Vol 107 ◽  
pp. 16-22 ◽  
Author(s):  
Axel Schubert ◽  
Eva Gentner ◽  
Kathrin Bohn ◽  
Maximilian Schwarz ◽  
Thomas Mertens ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Single Nucleotide Polymorphisms ◽  
Thymidine Kinase ◽  
Dna Polymerase ◽  
Herpes Simplex Virus Type ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Simplex Virus

scholarly journals Differential Mutation Patterns in Thymidine Kinase and DNA Polymerase Genes of Herpes Simplex Virus Type 1 Clones Passaged in the Presence of Acyclovir or Penciclovir

2003 ◽  
Vol 47 (5) ◽  
pp. 1707-1713 ◽  
Author(s):  
Tatsuo Suzutani ◽  
Ken Ishioka ◽  
Erik De Clercq ◽  
Kei Ishibashi ◽  
Hisatoshi Kaneko ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Dna Polymerase ◽  
Herpes Simplex Virus Type ◽  
Nucleotide Substitution ◽  
Single Nucleotide ◽  
Simplex Virus ◽  
Hsv 1

ABSTRACT A total of 21 clones of acyclovir (ACV)-resistant (ACVr) herpes simplex virus type 1 (HSV-1) and 23 clones of penciclovir (PCV)-resistant (PCVr) HSV-1, emerging during serial passages in the presence of ACV or PCV, were isolated under conditions excluding contamination of resistant mutants in the starting virus culture, and their mutations in the thymidine kinase (TK) and DNA polymerase (DNA Pol) genes were analyzed comparatively. Mutations in the TK genes from ACVr mutants consisted of 50% single nucleotide substitutions and 50% frameshift mutations, while the corresponding figures for the PCVr mutants were 4 and 96%, respectively (P < 0.001). Eight of the 21 ACVr clones, but none of the 23 PCVr clones, had mutations in DNA Pol. Only nucleotide substitution(s) could be detected in the DNA Pol gene, as the gene is essential for virus replication. Therefore, the results for the DNA Pol mutants are concordant with those for the TK mutants in that a single nucleotide substitution was commonly observed in the ACVr, but not in the PCVr, mutants. These results clearly point to differential mutation patterns between ACVr and PCVr HSV-1 clones.

Novel resistance-associated mutations of thymidine kinase and DNA polymerase genes of herpes simplex virus type 1 and type 2

2011 ◽  
Vol 16 (8) ◽  
pp. 1297-1308 ◽  
Author(s):  
Andreas Sauerbrei ◽  
Kathrin Bohn ◽  
Albert Heim ◽  
Jörg Hofmann ◽  
Benedikt Weiβbrich ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Dna Polymerase ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Simplex Virus

scholarly journals Role of the Carboxy Terminus of Herpes Simplex Virus Type 1 DNA Polymerase in its Interaction With UL42

1994 ◽  
Vol 75 (11) ◽  
pp. 3127-3135 ◽  
Author(s):  
H. S. Marsden ◽  
M. Murphy ◽  
G. L. McVey ◽  
K. A. MacEachran ◽  
A. M. Owsianka ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Dna Polymerase ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Carboxy Terminus ◽  
Simplex Virus
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