scholarly journals Hydrogen generation in a pressurized photobioreactor: Unexpected enhancement of biohydrogen production by the phototrophic bacterium Rhodobacter capsulatus

2019 ◽  
Vol 239 ◽  
pp. 635-643 ◽  
Author(s):  
Jean-Pierre Magnin ◽  
Jonathan Deseure
Keyword(s):  
Rhodobacter Capsulatus ◽  
Hydrogen Generation ◽  
Biohydrogen Production ◽  
Phototrophic Bacterium

High-level transcription of large gene regions: a novel T7 RNA-polymerase-based system for expression of functional hydrogenases in the phototrophic bacterium Rhodobacter capsulatus

2005 ◽  
Vol 33 (1) ◽  
pp. 56-58 ◽  
Author(s):  
T. Drepper ◽  
S. Arvani ◽  
F. Rosenau ◽  
S. Wilhelm ◽  
K.-E. Jaeger
Keyword(s):  
Rna Polymerase ◽  
Rhodobacter Capsulatus ◽  
Expression System ◽  
Active Form ◽  
T7 Rna Polymerase ◽  
High Level Synthesis ◽  
Phototrophic Bacterium ◽  
Large Gene ◽  
High Level ◽  
Complex Enzymes

High-level synthesis of complex enzymes like bacterial [NiFe] hydrogenases, in general, requires an expression system that allows concerted expression of a large number of genes. So far, it has not been possible to overproduce a hydrogenase in a stable and active form by using a customary expression system. Therefore we started to establish a new, T7-based expression system in the phototrophic bacterium Rhodobacter capsulatus. The beneficial properties of this bacterial host in combination with the unique capacity of T7 RNA polymerase to synthesize long transcripts will allow the high-level synthesis and assembly of active hydrogenase as well as other complex enzymes in the near future.

Biohydrogen production by Rhodobacter capsulatus on acetate at fluctuating temperatures

2010 ◽  
Vol 54 (5) ◽  
pp. 310-314 ◽  
Author(s):  
Ebru Özgür ◽  
Basar Uyar ◽  
Yavuz Öztürk ◽  
Meral Yücel ◽  
Ufuk Gündüz ◽  
...  
Keyword(s):  
Rhodobacter Capsulatus ◽  
Biohydrogen Production ◽  
Fluctuating Temperatures

Degradation of p -nitrophenol by the phototrophic bacterium Rhodobacter capsulatus

1997 ◽  
Vol 169 (1) ◽  
pp. 36-42 ◽  
Author(s):  
M. D. Roldán ◽  
R. Blasco ◽  
F. J. Caballero ◽  
F. Castillo
Keyword(s):  
Rhodobacter Capsulatus ◽  
Phototrophic Bacterium

Effect of inactivation of genes involved in ammonium regulation on the biohydrogen production of Rhodobacter capsulatus

2011 ◽  
Vol 36 (21) ◽  
pp. 13536-13546 ◽  
Author(s):  
Gülşah Pekgöz ◽  
Ufuk Gündüz ◽  
Inci Eroğlu ◽  
Meral Yücel ◽  
Kornél Kovács ◽  
...  
Keyword(s):  
Rhodobacter Capsulatus ◽  
Biohydrogen Production ◽  
Ammonium Regulation

No Correlation between Plasmid Content and Ability to Reduce Nitrate in Wild-Type Strains of Rhodobacter capsulatus

1991 ◽  
Vol 46 (7-8) ◽  
pp. 703-705 ◽  
Author(s):  
Astrid Witt ◽  
Jobst-Heinrich Klemme
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Rhodobacter Capsulatus ◽  
Reductase Activity ◽  
Large Plasmid ◽  
Wild Type ◽  
Plasmid Content ◽  
Phototrophic Bacterium ◽  
N Source ◽  
Type Strains

Patterns of endogenous plasmids and nitrate reductase activities were analyzed in the phototrophic bacterium Rhodobacter (Rb.) capsulatus. From 10 strains investigated (including a UV-induced plasmidless nit- mutant), 4 were unable to grow photosynthetically with nitrate as N-source and lacked nitrate reductase activity (nit strains). Irrespective of the nit phenotype, all wildtype strains contained at least one large plasmid with a size ranging from 93 to 134 kb. Thus, other than in plasmid- cured mutants (J. C. Willison, FEMS Microbiol. Lett. 66, 23-28[1990]), in wild-type strains of Rb. capsulatus the nit- character was not related to lack of endogenous plasmids.

Expression and characterization of the assimilatory NADH-nitrite reductase from the phototrophic bacterium Rhodobacter capsulatus E1F1

2006 ◽  
Vol 186 (4) ◽  
pp. 339-344 ◽  
Author(s):  
M. Francisca Olmo-Mira ◽  
Purificación Cabello ◽  
Carmen Pino ◽  
Manuel Martínez-Luque ◽  
David J. Richardson ◽  
...  
Keyword(s):  
Nitrite Reductase ◽  
Rhodobacter Capsulatus ◽  
Phototrophic Bacterium
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