scholarly journals Horseradish peroxidase-catalyzed oxidation of chlorophyll a with hydrogen peroxide

2010 ◽  
Vol 1797 (5) ◽  
pp. 531-542 ◽  
Author(s):  
Paavo H. Hynninen ◽  
Vesa Kaartinen ◽  
Erkki Kolehmainen
Keyword(s):  
Hydrogen Peroxide ◽  
Horseradish Peroxidase ◽  
Chlorophyll A ◽  
Catalyzed Oxidation

Horseradish peroxidase/hydrogen peroxide-catalyzed oxidation of VP16-213. Identification of a new metabolite

1985 ◽  
Vol 55 ◽  
pp. 215-224 ◽  
Author(s):  
Massimo Broggini ◽  
Cosmo Rossi ◽  
Emilio Benfenati ◽  
Maurizio D'Incalci ◽  
Roberto Fanelli ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Horseradish Peroxidase ◽  
Catalyzed Oxidation

scholarly journals In vitro studies of temperature and pH influence on chlorophyll degradation by horseradish peroxidase: Spectroscopic and HPLC studies

2014 ◽  
Vol 68 (2) ◽  
pp. 233-239 ◽  
Author(s):  
Sanja Petrovic ◽  
Sasa Savic ◽  
Dejan Markovic ◽  
Zivomir Petronijevic
Keyword(s):  
Hydrogen Peroxide ◽  
Horseradish Peroxidase ◽  
Chlorophyll A ◽  
Hplc Analysis ◽  
Ph Values ◽  
Ph Influence ◽  
Main Degradation Product ◽  
Different Temperatures ◽  
Influence Of Ph

In vitro chlorophyll a degradation by horseradish peroxidase in the presence of the resorcinol was investigated in this work, and the influence of pH and temperature was particularly studied. Chlorophyll a degradation was followed by UV-VIS and HPLC. Chlorophyll a was degraded when hydrogen peroxide was added into reaction mixture containing chlorophyll fraction, horseradish peroxidase, resorcinol and phosphate buffer. HPLC analysis has identified the main degradation product of chlorophyll a as 132 -hydroxychlorophyll a. The degradation was traced at different temperatures and pH values. The increasing temperatures led to increase of chlorophyll a degradation, with a maximum at 37 ?C. The degradation also increased with increasing pH values, reaching maximum at pH 6.

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