scholarly journals Site-directed mutagenesis of residues involved in proton translocation by Escherichia coli complex I

2012 ◽  
Vol 1817 ◽  
pp. S63 ◽  
Author(s):  
A.M. Wojciechowska ◽  
L.A. Sazanov
Keyword(s):  
Escherichia Coli ◽  
Complex I ◽  
Proton Translocation ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

scholarly journals Probing the mechanistic role of the long α‐helix in subunit L of respiratory Complex I from Escherichia coli by site‐directed mutagenesis

2011 ◽  
Vol 82 (5) ◽  
pp. 1086-1095 ◽  
Author(s):  
Galina Belevich ◽  
Juho Knuuti ◽  
Michael I. Verkhovsky ◽  
Mårten Wikström ◽  
Marina Verkhovskaya
Keyword(s):  
Escherichia Coli ◽  
Complex I ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Respiratory Complex ◽  
Respiratory Complex I ◽  
Α Helix

scholarly journals Overexpression, site-directed mutagenesis, and mechanism of Escherichia coli acid phosphatase

1992 ◽  
Vol 267 (32) ◽  
pp. 22830-22836 ◽  
Author(s):  
K Ostanin ◽  
E.H. Harms ◽  
P.E. Stevis ◽  
R Kuciel ◽  
M.M. Zhou ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Acid Phosphatase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

scholarly journals A genetically detoxified derivative of heat-labile Escherichia coli enterotoxin induces neutralizing antibodies against the A subunit.

1994 ◽  
Vol 180 (6) ◽  
pp. 2147-2153 ◽  
Author(s):  
M Pizza ◽  
M R Fontana ◽  
M M Giuliani ◽  
M Domenighini ◽  
C Magagnoli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cholera Toxin ◽  
Neutralizing Antibodies ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
E Coli ◽  
Heat Labile ◽  
A Subunit ◽  
Adp Ribosyltransferase ◽  
Derivatives Of

Escherichia coli enterotoxin (LT) and the homologous cholera toxin (CT) are A-B toxins that cause travelers' diarrhea and cholera, respectively. So far, experimental live and killed vaccines against these diseases have been developed using only the nontoxic B portion of these toxins. The enzymatically active A subunit has not been used because it is responsible for the toxicity and it is reported to induce a negligible titer of toxin neutralizing antibodies. We used site-directed mutagenesis to inactivate the ADP-ribosyltransferase activity of the A subunit and obtained nontoxic derivatives of LT that elicited a good titer of neutralizing antibodies recognizing the A subunit. These LT mutants and equivalent mutants of CT may be used to improve live and killed vaccines against cholera and enterotoxinogenic E. coli.

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