Design and biological assessment of membrane-tethering neuroprotective peptides derived from the pituitary adenylate cyclase-activating polypeptide type 1 receptor

2019 ◽  
Vol 1863 (11) ◽  
pp. 129398 ◽  
Author(s):  
Mathilde Poujol de Molliens ◽  
Priyanka Jamadagni ◽  
Myriam Létourneau ◽  
Dominic Devost ◽  
Terence E. Hébert ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Biological Assessment ◽  
Pituitary Adenylate Cyclase ◽  
Membrane Tethering

Mild deficits in mice lacking pituitary adenylate cyclase-activating polypeptide receptor type 1 (PAC1) performing on memory tasks

2000 ◽  
Vol 84 (1-2) ◽  
pp. 79-89 ◽  
Author(s):  
Magdalena Sauvage ◽  
Philippe Brabet ◽  
Florian Holsboer ◽  
Joel Bockaert ◽  
Thomas Steckler
Keyword(s):  
Adenylate Cyclase ◽  
Receptor Type ◽  
Pituitary Adenylate Cyclase

scholarly journals Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) and PACAP-Receptor Type 1 Expression in Rat and Human Placenta1

Endocrinology ◽  
2000 ◽  
Vol 141 (3) ◽  
pp. 1158-1167 ◽  
Author(s):  
Maria Lucia Scaldaferri ◽  
Andrea Modesti ◽  
Camilla Palumbo ◽  
Salvatore Ulisse ◽  
Andrea Fabbri ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Receptor Type ◽  
Pituitary Adenylate Cyclase

Identification of Binding Domains of Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) for its Type 1 Receptor by Photoaffinity Labelinga

1998 ◽  
Vol 865 (1 VIP, PACAP, A) ◽  
pp. 82-91 ◽  
Author(s):  
YONG-JIANG CAO ◽  
ELZBIETA KOJRO ◽  
MAREK JASIONOWSKI ◽  
LESZEK LANKIEWICZ ◽  
ZBIGNIEW GRZONKA ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Binding Domains ◽  
Pituitary Adenylate Cyclase

scholarly journals Pituitary Adenylate Cyclase-activating Polypeptide Type 1 Receptor (PAC1) Gene Is Suppressed by Transglutaminase 2 Activation

2013 ◽  
Vol 288 (45) ◽  
pp. 32720-32730 ◽  
Author(s):  
Ayako Miura ◽  
Yuki Kambe ◽  
Kazuhiko Inoue ◽  
Hideki Tatsukawa ◽  
Takashi Kurihara ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Transglutaminase 2 ◽  
Pituitary Adenylate Cyclase

scholarly journals Multiple splice variants of the pituitary adenylate cyclase-activating polypeptide type 1 receptor detected by RT-PCR in single rat pituitary cells

1998 ◽  
Vol 21 (2) ◽  
pp. 109-120 ◽  
Author(s):  
L Bresson-Bepoldin ◽  
MC Jacquot ◽  
W Schlegel ◽  
SR Rawlings
Keyword(s):  
Alternative Splicing ◽  
Adenylate Cyclase ◽  
Single Cell ◽  
Splice Variant ◽  
Cell Biology ◽  
Splice Variants ◽  
Pituitary Cells ◽  
Rt Pcr ◽  
Pituitary Adenylate Cyclase

Alternative splicing of the rat type 1 pituitary adenylate cyclase-activating polypeptide (PACAP) receptor (PVR1) produces variants that couple either to both adenylyl cyclase (AC) and phospholipase C (PLC) (PVR1 short, PVR1 hop, PVR1 hiphop), or to AC alone (PVR1 hip). We have previously shown that populations of clonal alphaT3-1 gonadotrophs express PVR1 hop and PVR1 short mRNAs, whereas clonal GH4C1 somatotrophs do not. Here we have used the single cell RT-PCR technique to investigate whether normal rat gonadotrophs and somatotrophs express PVR1 mRNA, whether a single cell co-expresses multiple splice variant forms, and whether differential PVR1 mRNA expression correlates with differences in PACAP-stimulated Ca2+ signalling. We found that individual rat gonadotrophs expressed mRNA either for PVR1 hop, for PVR1 short, or co-expressed the two forms. Although we found no differences between the splice variant(s) expressed and the characteristics of PACAP-stimulated Ca2+ responses, the expression of PVR1 mRNA is consistent with the known PACAP stimulation of the PLC system in gonadotrophs. Individual rat somatotrophs also expressed PVR1 hop or PVR1 short (but not PVR1 hip) mRNAs although these forms were never co-expressed. The expression of PVR1 mRNA in somatotrophs can explain in part the activation by PACAP of the AC system in such cells. In conclusion, the single cell RT-PCR technique was used to demonstrate expression of multiple PVR1 splice variants in single identified pituitary cells. These findings open up important questions on the role of alternative splicing in cell biology.

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