The intra-mitochondrial cytochrome c distribution varies correlated to the formation of a complex between VDAC and the adenine nucleotide translocase: this affects Bax-dependent cytochrome c release

Platycodon grandiflorum (Jacq.) A. DC., a Chinese food and medicine, has been used as expectorant traditionally. The present study aimed to investigate the effect of Platycodon grandiflorum extract (PGE) on SKOV3 ovarian cancer cells. 3-(4,5- dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay was used to monitor cell numbers, Annexin-V/propidium iodide (PI) staining, RT-PCR and Western blot were used to examine cell apoptosis, caspases activation. Bcl-2 and Bax expressions and mitochondrial cytochrome c release. Our result showed that PGE-induced apoptosis was associated with activation of caspase-3, -8 and -9, down-regulation of Bcl-2, up-regulation of Bax and release of mitochondrial cytochrome c to cytosol. The data indicate that PGE may have anti-tumor effect mainly via caspase-3 and caspase-9 dependent apoptotic pathway.

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Antioxidants prevent UV-induced apoptosis by inhibiting mitochondrial cytochrome c release and caspase activation in Spodoptera frugiperda (Sf9) cells

Cell Biology International ◽

10.1016/s1065-6995(03)00071-4 ◽

2003 ◽

Vol 27 (6) ◽

pp. 483-490 ◽

Cited By ~ 29

Author(s):

M Mohan

Keyword(s):

Cytochrome C ◽

Spodoptera Frugiperda ◽

Sf9 Cells ◽

Mitochondrial Cytochrome ◽

Caspase Activation ◽

Cytochrome C Release ◽

Induced Apoptosis ◽

Mitochondrial Cytochrome C

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Regional expression of Bcl-2 mRNA and mitochondrial cytochrome c release after experimental brain injury in the rat

Brain Research ◽

10.1016/s0006-8993(01)02379-4 ◽

2001 ◽

Vol 903 (1-2) ◽

pp. 45-52 ◽

Cited By ~ 14

Author(s):

Gao-Xiang Dong ◽

Dev K Singh ◽

Peter Dendle ◽

Renuka M Prasad

Keyword(s):

Brain Injury ◽

Cytochrome C ◽

Mitochondrial Cytochrome ◽

Cytochrome C Release ◽

Mitochondrial Cytochrome C

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Cleavage of BID during cytotoxic drug and UV radiation-induced apoptosis occurs downstream of the point of Bcl-2 action and is catalysed by caspase-3: a potential feedback loop for amplification of apoptosis-associated mitochondrial cytochrome c release

Cell Death and Differentiation ◽

10.1038/sj.cdd.4400689 ◽

2000 ◽

Vol 7 (6) ◽

pp. 556-565 ◽

Cited By ~ 198

Author(s):

E A Slee ◽

S A Keogh ◽

S J Martin

Keyword(s):

Cytochrome C ◽

Uv Radiation ◽

Feedback Loop ◽

Caspase 3 ◽

Cytotoxic Drug ◽

Mitochondrial Cytochrome ◽

Cytochrome C Release ◽

Radiation Induced ◽

Induced Apoptosis ◽

Mitochondrial Cytochrome C

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Mitochondrial cytochrome c release is caspase-dependent and does not involve mitochondrial permeability transition in didemnin B-induced apoptosis

Oncogene ◽

10.1038/sj.onc.1204545 ◽

2001 ◽

Vol 20 (30) ◽

pp. 4085-4094 ◽

Cited By ~ 26

Author(s):

David R Grubb ◽

Jennifer D Ly ◽

François Vaillant ◽

Karina L Johnson ◽

Alfons Lawen

Keyword(s):

Cytochrome C ◽

Mitochondrial Permeability Transition ◽

Permeability Transition ◽

Didemnin B ◽

Mitochondrial Cytochrome ◽

Cytochrome C Release ◽

Mitochondrial Permeability ◽

Induced Apoptosis ◽

Mitochondrial Cytochrome C

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Altered proteasomal function due to the expression of polyglutamine-expanded truncated N-terminal huntingtin induces apoptosis by caspase activation through mitochondrial cytochrome c release

Human Molecular Genetics ◽

10.1093/hmg/10.10.1049 ◽

2001 ◽

Vol 10 (10) ◽

pp. 1049-1059 ◽

Cited By ~ 303

Author(s):

N. R. Jana

Keyword(s):

Cytochrome C ◽

Mitochondrial Cytochrome ◽

Caspase Activation ◽

Cytochrome C Release ◽

Mitochondrial Cytochrome C

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Cytochrome c release into cytosol with subsequent caspase activation during warm ischemia in rat liver

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.2001.281.4.g1115 ◽

2001 ◽

Vol 281 (4) ◽

pp. G1115-G1123 ◽

Cited By ~ 35

Author(s):

Junpei Soeda ◽

Shinichi Miyagawa ◽

Kenji Sano ◽

Junya Masumoto ◽

Shun'Ichiro Taniguchi ◽

...

Keyword(s):

Cytochrome C ◽

Dna Fragmentation ◽

Warm Ischemia ◽

Terminal Deoxynucleotidyl Transferase ◽

Dependent Manner ◽

Mitochondrial Cytochrome ◽

Caspase Activation ◽

Cytochrome C Release ◽

Apoptotic Pathway ◽

Mitochondrial Cytochrome C

Apoptosis plays an important role in liver ischemia and reperfusion (I/R) injury. However, the molecular basis of apoptosis in I/R injury is poorly understood. The aims of this study were to ascertain when and how apoptotic signal transduction occurs in I/R injury. The apoptotic pathway in rats undergoing 90 min of warm ischemia with reperfusion was compared with that of rats undergoing prolonged ischemia alone. During ischemia, mitochondrial cytochrome c was released into the cytosol in a time-dependent manner in hepatocytes and sinusoidal endothelial cells, and caspase-3 and an inhibitor of caspase-activated DNase were cleaved. However, apoptotic manifestation and DNA fragmentation were not observed. After reperfusion, nuclear condensation, cells positive for terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling, and DNA fragmentation were observed and caspase-8 and Bid cleavage occurred. In contrast, prolonged ischemia alone induced necrosis rather than apoptosis. In summary, our results show that release of mitochondrial cytochrome c and caspase activation proceed during ischemia, although apoptosis is manifested after reperfusion.

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