Label-free detection of small-molecule binding to a GPCR in the membrane environment

2015 ◽  
Vol 1854 (8) ◽  
pp. 979-986 ◽  
Author(s):  
Roland G. Heym ◽  
Wilfried B. Hornberger ◽  
Viktor Lakics ◽  
Georg C. Terstappen
Keyword(s):  
Small Molecule ◽  
Label Free ◽  
Label Free Detection

scholarly journals A Simple Displacement Aptamer Assay on Resistive Pulse Sensor for Small Molecule Detection

2020 ◽  
Author(s):  
Rhushabh Maugi ◽  
bernadette gamble ◽  
david bunka ◽  
Mark Platt
Keyword(s):  
Small Molecules ◽  
Small Molecule ◽  
Label Free ◽  
Resistive Pulse ◽  
Pulse Sensor ◽  
Label Free Detection ◽  
Ssdna Aptamer ◽  
Aptamer Assay ◽  
Small Molecule Detection ◽  
Surface Changes

A universal aptamer-based sensing strategy is proposed using DNA modified nanocarriers and Resistive Pulse Sensing for the rapid and label free detection of small molecules. The surface of a magnetic nanocarrier was first modified with a ssDNA aka linker which is designed to be partially complimentary in sequence to a ssDNA aptamer. The aptamer and linker form a stable dsDNA complex on the nanocarriers surface. Upon the addition of the target molecule, a conformational change takes place where the aptamer preferentially binds to the target over the linker; causing the aptamer to be released into solution. The RPS measures the change in velocity of the nanocarrier as its surface changes from dsDNA to ssDNA, and its velocity is used as a proxy for the concentration of the target. We illustrate the versatility of the assay by demonstrating the detection of the antibiotic Moxifloxacin, and chemotherapeutics Imatinib and Irinotecan.

scholarly journals An aptameric graphene nanosensor for label-free detection of small-molecule biomarkers

2015 ◽  
Vol 71 ◽  
pp. 222-229 ◽  
Author(s):  
Cheng Wang ◽  
Jinho Kim ◽  
Yibo Zhu ◽  
Jaeyoung Yang ◽  
Gwan-Hyoung Lee ◽  
...  
Keyword(s):  
Small Molecule ◽  
Label Free ◽  
Label Free Detection

Size does matter! Label-free detection of small molecule–protein interaction

2014 ◽  
Vol 406 (17) ◽  
pp. 4033-4051 ◽  
Author(s):  
Peter Fechner ◽  
Oliver Bleher ◽  
Melanie Ewald ◽  
Kathrin Freudenberger ◽  
Dominik Furin ◽  
...  
Keyword(s):  
Small Molecule ◽  
Protein Interaction ◽  
Label Free ◽  
Label Free Detection

scholarly journals A Simple Displacement Aptamer Assay on Resistive Pulse Sensor for Small Molecule Detection

2020 ◽  
Author(s):  
Rhushabh Maugi ◽  
bernadette gamble ◽  
david bunka ◽  
Mark Platt
Keyword(s):  
Small Molecules ◽  
Small Molecule ◽  
Label Free ◽  
Resistive Pulse ◽  
Pulse Sensor ◽  
Label Free Detection ◽  
Ssdna Aptamer ◽  
Aptamer Assay ◽  
Small Molecule Detection ◽  
Surface Changes

A universal aptamer-based sensing strategy is proposed using DNA modified nanocarriers and Resistive Pulse Sensing for the rapid and label free detection of small molecules. The surface of a magnetic nanocarrier was first modified with a ssDNA aka linker which is designed to be partially complimentary in sequence to a ssDNA aptamer. The aptamer and linker form a stable dsDNA complex on the nanocarriers surface. Upon the addition of the target molecule, a conformational change takes place where the aptamer preferentially binds to the target over the linker; causing the aptamer to be released into solution. The RPS measures the change in velocity of the nanocarrier as its surface changes from dsDNA to ssDNA, and its velocity is used as a proxy for the concentration of the target. We illustrate the versatility of the assay by demonstrating the detection of the antibiotic Moxifloxacin, and chemotherapeutics Imatinib and Irinotecan.

scholarly journals Label-free detection of small-molecule-protein interactions by using nanowire nanosensors

2005 ◽  
Vol 102 (9) ◽  
pp. 3208-3212 ◽  
Author(s):  
W. U. Wang ◽  
C. Chen ◽  
K.-h. Lin ◽  
Y. Fang ◽  
C. M. Lieber
Keyword(s):  
Small Molecule ◽  
Protein Interactions ◽  
Label Free ◽  
Label Free Detection
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