Bacterial expression and re-engineering of Gaussia princeps luciferase and its use as a reporter protein

2015 ◽  
Vol 1854 (10) ◽  
pp. 1392-1399 ◽  
Author(s):  
Nan Wu ◽  
Tharangani Rathnayaka ◽  
Yutaka Kuroda
Keyword(s):  
Bacterial Expression ◽  
Reporter Protein ◽  
Gaussia Princeps

Recovery of Recombinant Rotavirus Expressing Fluorescent Reporter Protein

2019 ◽  
Author(s):  
Asha Philip ◽  
Jin Dai ◽  
Sarah Katen ◽  
John Patton
Keyword(s):  
Reporter Protein ◽  
Fluorescent Reporter

Conditions Resulting in Formation of Properly Assembled Retroviral Capsids within Inclusion Bodies of Escherichia coli

1999 ◽  
Vol 64 (8) ◽  
pp. 1348-1356 ◽  
Author(s):  
Michaela Rumlová-Kliková ◽  
Iva Pichová ◽  
Eric Hunter ◽  
Tomáš Ruml
Keyword(s):  
Escherichia Coli ◽  
Inclusion Bodies ◽  
Amorphous Material ◽  
Bacterial Expression ◽  
Biologically Active ◽  
Capsid Assembly ◽  
Cultivation Medium ◽  
Viral Particles ◽  
Induction Temperature

It has been generally accepted that inclusion bodies (IBs) formed in Escherichia coli consist of non-biologically active aggregated proteins, which are stabilized by non-productive interactions. We show here that bacterial expression of a retroviral capsid polyprotein results in formation of insoluble IBs that contain fully assembled viral particles connected with amorphous material. The efficiency of IBs formation and capsid assembly was not significantly affected by changes in induction temperature, pH of cultivation medium or the level of expression.

Improved soluble bacterial expression and properties of the recombinant flavonoid glucosyltransferase UGT73G1 from Allium cepa

2017 ◽  
Vol 255 ◽  
pp. 9-15 ◽  
Author(s):  
Ruxin Cai ◽  
Caihong Chen ◽  
Yan Li ◽  
Kaiyan Sun ◽  
Fangfang Zhou ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Bacterial Expression

Detection of benzene derivatives by recombinant E. coli with Ps promoter and GFP as a reporter protein

2003 ◽  
Vol 15 (3) ◽  
pp. 193-197 ◽  
Author(s):  
Shinya Ikeno ◽  
Chiaki Ogino ◽  
Takeo Ito ◽  
Nobuaki Shimizu
Keyword(s):  
Benzene Derivatives ◽  
Reporter Protein ◽  
E Coli

A reporter protein for meat integrity

Meat Science ◽  
1998 ◽  
Vol 49 (3) ◽  
pp. 347-356
Author(s):  
Vincenzo Salfi ◽  
Ottavio Portanti
Keyword(s):  
Reporter Protein

Co-expression of a scFv antibody fragment and a reporter protein using lentiviral shuttle plasmid containing a self-processing furin-2A sequence

2013 ◽  
Vol 397 (1-2) ◽  
pp. 61-65 ◽  
Author(s):  
Sarah L. Appleby ◽  
Yazad Irani ◽  
Lauren A. Mortimer ◽  
Helen M. Brereton ◽  
Sonja Klebe ◽  
...  
Keyword(s):  
Antibody Fragment ◽  
Scfv Antibody ◽  
Reporter Protein ◽  
Shuttle Plasmid ◽  
Scfv Antibody Fragment
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