Conditions Resulting in Formation of Properly Assembled Retroviral Capsids within Inclusion Bodies of Escherichia coli
1999 ◽
Vol 64
(8)
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pp. 1348-1356
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Keyword(s):
It has been generally accepted that inclusion bodies (IBs) formed in Escherichia coli consist of non-biologically active aggregated proteins, which are stabilized by non-productive interactions. We show here that bacterial expression of a retroviral capsid polyprotein results in formation of insoluble IBs that contain fully assembled viral particles connected with amorphous material. The efficiency of IBs formation and capsid assembly was not significantly affected by changes in induction temperature, pH of cultivation medium or the level of expression.
2003 ◽
Vol 31
(2)
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pp. 271-275
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Keyword(s):
2015 ◽
Vol 110
◽
pp. 79-88
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Keyword(s):
2011 ◽
Vol 77
(1)
◽
pp. 98-103
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2015 ◽
Vol 115
◽
pp. 95-101
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Keyword(s):
Keyword(s):
2005 ◽
Vol 27
(20)
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pp. 1559-1564
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1968 ◽
Vol 26
◽
pp. 222-223
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1980 ◽
Vol 38
◽
pp. 486-487
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