Nanosecond pulsed electric fields (nsPEFs) activate intrinsic caspase-dependent and caspase-independent cell death in Jurkat cells

2012 ◽  
Vol 421 (4) ◽  
pp. 808-812 ◽  
Author(s):  
Wei Ren ◽  
Nova M. Sain ◽  
Stephen J. Beebe
Keyword(s):  
Cell Death ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Jurkat Cells ◽  
Nanosecond Pulsed Electric Fields

Changes in protein expression of U937 and Jurkat cells exposed to nanosecond pulsed electric fields

2013 ◽  
Author(s):  
Erick K. Moen ◽  
Caleb C. Roth ◽  
Caesar Cerna ◽  
Larry Estalck ◽  
Gerald Wilmink ◽  
...  
Keyword(s):  
Protein Expression ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Jurkat Cells ◽  
Nanosecond Pulsed Electric Fields

scholarly journals Electroperturbation of DNA in Jurkat Cells Under Nanosecond Pulsed Electric Fields

2011 ◽  
Vol 100 (3) ◽  
pp. 78a
Author(s):  
Stefania Romeo ◽  
Olga Zeni ◽  
Maria Rosaria Scarfi ◽  
Maurizio Sarti ◽  
Anna Sannino ◽  
...  
Keyword(s):  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Jurkat Cells ◽  
Nanosecond Pulsed Electric Fields

Cell Death Induced by Nanosecond Pulsed Electric Fields and its Dependence on Pulse Duration

2018 ◽  
Vol 101 (3) ◽  
pp. 38-48 ◽  
Author(s):  
RYUYA ADACHI ◽  
SHOTA HATAYAMA ◽  
NOBUAKI OHNISHI ◽  
SUNAO KATSUKI ◽  
TOSHIAKI WADA ◽  
...  
Keyword(s):  
Cell Death ◽  
Pulse Duration ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Nanosecond Pulsed Electric Fields

Cell Death induced by Nanosecond Pulsed Electric Fields and its Dependence on Pulse Duration

2017 ◽  
Vol 137 (6) ◽  
pp. 320-327
Author(s):  
Ryuta Andachi ◽  
Shota Hatayama ◽  
Nobuaki Ohnishi ◽  
Sunao Katsuki ◽  
Toshiaki Wada ◽  
...  
Keyword(s):  
Cell Death ◽  
Pulse Duration ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Nanosecond Pulsed Electric Fields

scholarly journals Towards Solid Tumor Treatment by Nanosecond Pulsed Electric Fields

2009 ◽  
Vol 8 (4) ◽  
pp. 289-306 ◽  
Author(s):  
Axel T. Esser ◽  
Kyle C. Smith ◽  
T. R. Gowrishankar ◽  
James C. Weaver
Keyword(s):  
Cell Death ◽  
Electric Field ◽  
Spatial Scale ◽  
Electric Fields ◽  
Solid Tumor ◽  
Irreversible Electroporation ◽  
Pulsed Electric Fields ◽  
Underlying Mechanism ◽  
Tumor Ablation ◽  
Nanosecond Pulsed Electric Fields

Local and drug-free solid tumor ablation by large nanosecond pulsed electric fields leads to supra-electroporation of all cellular membranes and has been observed to trigger nonthermal cell death by apoptosis. To establish pore-based effects as the underlying mechanism inducing apoptosis, we use a multicellular system model (spatial scale 100 μm) that has irregularly shaped liver cells and a multiscale liver tissue model (spatial scale 200 mm). Pore histograms for the multicellular model demonstrate the presence of only nanometer-sized pores due to nanosecond electric field pulses. The number of pores in the plasma membrane is such that the average tissue conductance during nanosecond electric field pulses is even higher than for longer irreversible electroporation pulses. It is shown, however, that these nanometer-sized pores, although numerous, only significantly change the permeability of the cellular membranes to small ions, but not to larger molecules. Tumor ablation by nanosecond pulsed electric fields causes small to moderate temperature increases. Thus, the underlying mechanism(s) that trigger cell death by apoptosis must be non-thermal electrical interactions, presumably leading to different ionic and molecular transport than for much longer irreversible electroporation pulses.

Nanosecond pulsed electric fields modulate the expression of Fas/CD95 death receptor pathway regulators in U937 and Jurkat Cells

APOPTOSIS ◽  
2014 ◽  
Vol 19 (12) ◽  
pp. 1755-1768 ◽  
Author(s):  
Larry E. Estlack ◽  
Caleb C. Roth ◽  
Gary L. Thompson ◽  
William A. Lambert ◽  
Bennett L. Ibey
Keyword(s):  
Electric Fields ◽  
Death Receptor ◽  
Pulsed Electric Fields ◽  
Jurkat Cells ◽  
Death Receptor Pathway ◽  
Nanosecond Pulsed Electric Fields

Plasma Membrane Charging of Jurkat Cells by Nanosecond Pulsed Electric Fields

2007 ◽  
Author(s):  
Juergen F. Kolb ◽  
Jody A. White ◽  
Uwe Pliquett ◽  
Richard Nuccitelli ◽  
Karl H. Schoenbach ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Jurkat Cells ◽  
Nanosecond Pulsed Electric Fields

Plasma membrane charging of Jurkat cells by nanosecond pulsed electric fields

2011 ◽  
Vol 40 (8) ◽  
pp. 947-957 ◽  
Author(s):  
Jody A. White ◽  
Uwe Pliquett ◽  
Peter F. Blackmore ◽  
Ravindra P. Joshi ◽  
Karl H. Schoenbach ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Jurkat Cells ◽  
Nanosecond Pulsed Electric Fields
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