Pig sperm membrane microdomains contain a highly glycosylated 15–25-kDa wheat germ agglutinin-binding protein

A new stalk-specific wheat germ agglutinin (WGA) binding protein, wst34, has been identified in Dictyostelium discoideum and purified by the use of preparative sodium dodecyl sulfate - polyacrylamide gel electrophoresis and a WGA-affinity column. In normal development, wst34 appears during culmination and is maintained in stalk cells. It has a molecular mass of 34 kilodaltons and a pI value of 5.5–6.5. A polyclonal antiserum raised against stalk cell proteins of Dictyostelium mucoroides recognizes wst34 in western blots of D. discoideum proteins.Key words: Dictyostelium discoideum, Dictyostelium mucoroides, wheat germ agglutinin.

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Wheat germ agglutinin-binding protein changes in highly malignant Friend leukemia cells metastasizing to the liver

Clinical & Experimental Metastasis ◽

10.1007/bf01760571 ◽

1988 ◽

Vol 6 (5) ◽

pp. 347-362 ◽

Cited By ~ 6

Author(s):

Giuliano Elia ◽

Maria Ferrantini ◽

Filippo Belardelli ◽

Enrico Proietti ◽

Ion Gresser ◽

...

Keyword(s):

Wheat Germ Agglutinin ◽

Wheat Germ ◽

Binding Protein ◽

Leukemia Cells ◽

Friend Leukemia

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Purification and properties of a stalk-specific wheat germ agglutinin binding protein, Dr-st36, in the cellular slime mould, Dictyostelium rosarium

Comparative Biochemistry and Physiology Part B Comparative Biochemistry ◽

10.1016/0305-0491(93)90172-2 ◽

1993 ◽

Vol 105 (1) ◽

pp. 87-89

Author(s):

Yuzuru Kubohara

Keyword(s):

Wheat Germ Agglutinin ◽

Wheat Germ ◽

Binding Protein ◽

Cellular Slime Mould ◽

Slime Mould ◽

Purification And Properties ◽

Cellular Slime

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Failure of 6D1 or Exposure of Platelets to ADP to Affect Agglutination Induced by Wheat Germ Agglutinin

Thrombosis and Haemostasis ◽

10.1055/s-0038-1646911 ◽

1989 ◽

Vol 62 (02) ◽

pp. 815 ◽

Cited By ~ 1

Author(s):

Marjorie B Zucker ◽

Robert A Grant ◽

Evelyn A Mauss

Keyword(s):

Wheat Germ Agglutinin ◽

Wheat Germ

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Transport Characteristics of Wheat Germ Agglutinin-Modified Insulin-Liposomes and Solid Lipid Nanoparticles in a Perfused Rat Intestinal Model

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2006.425 ◽

2006 ◽

Vol 6 (9) ◽

pp. 2959-2966 ◽

Cited By ~ 16

Author(s):

Na Zhang ◽

Qineng Ping ◽

Guihua Huang ◽

Xiuzhen Han ◽

Yanna Cheng ◽

...

Keyword(s):

Solid Lipid Nanoparticles ◽

Wheat Germ Agglutinin ◽

Wheat Germ ◽

Serum Insulin ◽

Lipid Nanoparticles ◽

Solid Lipid ◽

Mucosal Absorption ◽

Perfusion Experiment ◽

Absorption Sites

Wheat germ agglutinin (WGA) modified liposomes and solid lipid nanoparticles (SLNs) were evaluated for improving intestinal absorption of insulin. In an in situ local intestinal perfusion experiment, formulations containing 100 IU/kg insulin were administered to the duodenum, jejunum, and ileum of fasted rats. As hypothesized, ileum was the best intestinal location for the absorption of insulin-containing liposomes. Serum insulin concentrations decreased for the various formulations in different absorption sites according to the following trends: Duodenum > ileum > jejunum for WGA-modified insulin-containing liposomes; duodenum > jejunum > ileum for WGA-modified insulin-containing SLNs; ileum > jejunum > duodenum for insulin-containing liposomes; ileum > duodenum > jejunum for insulin-containing SLNs; and duodenum ≥ ileum > jejunum for aqueous solution of insulin. These results imply that the nanoparticle type and delivery site were important factors with respect to increasing the bioavailability of insulin following oral administration. The proteolytic degradation as well as the epithelial permeability were primary determinants influcing insulin mucosal absorption.

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