Albumin overload induces expression of hypoxia-inducible factor 1α and its target genes in HK-2 human renal proximal tubular cell line

2013 ◽  
Vol 434 (3) ◽  
pp. 670-675 ◽  
Author(s):  
Junya Nagai ◽  
Ayaka Yamamoto ◽  
Ryoko Yumoto ◽  
Mikihisa Takano
Keyword(s):  
Cell Line ◽  
Target Genes ◽  
Hypoxia Inducible Factor ◽  
Proximal Tubular Cell ◽  
Tubular Cell ◽  
Hypoxia Inducible Factor 1Α ◽  
Renal Proximal Tubular Cell ◽  
Proximal Tubular ◽  
Renal Proximal Tubular

Novel human renal proximal tubular cell line for the production of complex proteins

2014 ◽  
Vol 176 ◽  
pp. 29-39 ◽  
Author(s):  
Lukas Fliedl ◽  
Gabriele Manhart ◽  
Florian Kast ◽  
Hermann Katinger ◽  
Renate Kunert ◽  
...  
Keyword(s):  
Cell Line ◽  
Proximal Tubular Cell ◽  
Tubular Cell ◽  
Renal Proximal Tubular Cell ◽  
Proximal Tubular ◽  
Renal Proximal Tubular

Comparison of the novel HK-2 human renal proximal tubular cell line with the standard LLC-PK1 cell line in studying drug-induced nephrotoxicity

2010 ◽  
Vol 88 (4) ◽  
pp. 448-455 ◽  
Author(s):  
Patrina Gunness ◽  
Katarina Aleksa ◽  
Kazuhiro Kosuge ◽  
Shinya Ito ◽  
Gideon Koren
Keyword(s):  
Cell Line ◽  
Proximal Tubular Cell ◽  
Tubular Cell ◽  
In Vitro Toxicity ◽  
Drug Induced ◽  
Toxicity Studies ◽  
Renal Proximal Tubular Cell ◽  
Proximal Tubular ◽  
Renal Proximal Tubular

Established cell lines are widely used as in vitro models in toxicology studies. The choice of an appropriate cell line is critical when performing studies to elucidate drug-induced toxicity in humans. The porcine renal proximal tubular cell line LLC-PK1 is routinely used to study the nephrotoxic effects of drugs in humans. However, there are significant interspecies differences in drug pharmacokinetics and pharmacodynamics. The objective of this study was to determine whether the human renal proximal tubular cell line HK-2 is an acceptable model to use when performing in vitro toxicity studies to predict effects in humans. We examined 2 nephrotoxic agents, ifosfamide (IFO) and acyclovir, that exhibit different clinical nephrotoxic patterns. HK-2 cells metabolized IFO to its nephrotoxic metabolite, chloroacetaldehyde (CAA). Acyclovir induced a concentration-dependent decrease in HK-2 cell viability, suggesting that acyclovir may induce direct insult to renal proximal tubular cells. The results support clinical pathology data in humans and suggest that HK-2 cells are a suitable model to use in in vitro toxicity studies to determine drug-induced nephrotoxicity in humans.

Sign in / Sign up

Export Citation Format

Share Document