Two conserved amino acids of juxtaposed domains of a ribosomal maturation protein CgtA sustain its optimal GTPase activity

2015 ◽  
Vol 461 (4) ◽  
pp. 636-641 ◽  
Author(s):  
Ananya Chatterjee ◽  
Partha P. Datta
Keyword(s):  
Amino Acids ◽  
Gtpase Activity ◽  
Maturation Protein ◽  
Conserved Amino Acids

scholarly journals Molecular cloning and characterization of ornithine decarboxylase cDNA of the nematode Panagrellus redivivus

1995 ◽  
Vol 308 (2) ◽  
pp. 635-640 ◽  
Author(s):  
H von Besser ◽  
G Niemann ◽  
B Domdey ◽  
R D Walter
Keyword(s):  
Amino Acids ◽  
Active Sites ◽  
Cdna Clones ◽  
Degenerate Primers ◽  
Calculated Molecular Mass ◽  
Panagrellus Redivivus ◽  
Free Living Nematode ◽  
Mixed Stage ◽  
Conserved Amino Acids

In a PCR with degenerate primers encoding highly conserved amino acids within ornithine decarboxylases (ODCs) of several organisms, a fragment of the ODC gene of the free-living nematode Panagrellus redivivus was isolated. Northern blot analysis revealed a single 1.7 kb transcript in a mixed-stage population of animals. From this RNA source, a cDNA library was constructed and screened with the PCR fragment. Several cDNA clones were isolated, one of which encodes the complete 435-amino-acid ODC enzyme with a calculated molecular mass of 47.1 kDa. The P. redivivus ODC possesses 126 of the 136 highly conserved amino acids in the enzymes from fungi, invertebrates and vertebrates. Functional amino acids are conserved, suggesting that the two active sites of the P. redivivus ODC are formed at the interface of a homodimer, as described for mammalian ODCs.

scholarly journals Impact of Mutations in Highly Conserved Amino Acids of the HIV-1 Gag-p24 and Env-gp120 Proteins on Viral Replication in Different Genetic Backgrounds

PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e94240 ◽  
Author(s):  
Yi Liu ◽  
Ushnal Rao ◽  
Jan McClure ◽  
Philip Konopa ◽  
Siriphan Manocheewa ◽  
...  
Keyword(s):  
Amino Acids ◽  
Viral Replication ◽  
Conserved Amino Acids ◽  
Hiv 1
Sign in / Sign up

Export Citation Format

Share Document