Extraction, purification and characterization of a thermally stable aspartic protease from freshwater shrimp Gammarus sp. with a high catalytic efficiency

2021 ◽  
pp. 102224
Author(s):  
Soheila Mohammadi ◽  
Mohammad Ali Azadi ◽  
Roohullah Hemmati ◽  
Ahmad Homaei
Keyword(s):  
Catalytic Efficiency ◽  
Aspartic Protease ◽  
Thermally Stable ◽  
Freshwater Shrimp ◽  
Purification And Characterization

Purification and characterization of a psychrophilic catalase from Antarctic Bacillus

2008 ◽  
Vol 54 (10) ◽  
pp. 823-828 ◽  
Author(s):  
Wei Wang ◽  
Mi Sun ◽  
Wanshun Liu ◽  
Bin Zhang
Keyword(s):  
Activation Energy ◽  
Low Temperatures ◽  
Catalytic Efficiency ◽  
Small Subunit ◽  
Purification And Characterization ◽  
Cold Adapted ◽  
Optimal Activity ◽  
Ph Range ◽  
Antarctic Seawater

Catalase from Bacillus sp. N2a (BNC) isolated from Antarctic seawater was purified to homogeneity. BNC has a molecular mass of about 230 kDa and is composed of four identical subunits of 56 kDa. The catalase showed optimal activity at 25 °C and at a pH range of 6–11. The enzyme could be inhibited by azide, hydroxylamine, and mercaptoethanol. These characteristics suggested that BNC is a small-subunit monofunctional catalase. The activation energy of BNC was 13 kJ/mol and the apparent kcat/Km values were 3.6 × 106 and 4 × 106 L·mol–1·s–1 at 4 and 25 °C, respectively. High catalytic efficiency of BNC at low temperatures enables this bacterium to scavenge H2O2 efficiently. BNC exhibited activation energy, catalytic efficiency, and thermostability comparable with some mesophilic homologues. Such similarity of enzymatic characteristics to mesophilic homologues, although uncommon among the cold-adapted enzymes in general, has also been observed in other psychrophilic small-subunit monofunctional catalases.

Purification and Characterization of Aspartic Protease Derived from Sf9 Insect Cells

2010 ◽  
Vol 74 (10) ◽  
pp. 2154-2157 ◽  
Author(s):  
Takeshi GOTOH ◽  
Hiroki ONO ◽  
Ken-Ichi KIKUCHI ◽  
Satoru NIRASAWA ◽  
Saori TAKAHASHI
Keyword(s):  
Insect Cells ◽  
Aspartic Protease ◽  
Purification And Characterization ◽  
Sf9 Insect Cells

Bovine Leukemia Virus: Purification and Characterization of the Aspartic Protease

Virology ◽  
1993 ◽  
Vol 193 (2) ◽  
pp. 680-689 ◽  
Author(s):  
Armelle Menard ◽  
Robert Z. Mamoun ◽  
Serge Geoffre ◽  
Michel Castroviejo ◽  
Sophie Raymond ◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Aspartic Protease ◽  
Purification And Characterization ◽  
Virus Purification

Purification and characterization of an aspartic protease from potato leaves

2001 ◽  
Vol 112 (3) ◽  
pp. 321-326 ◽  
Author(s):  
María G. Guevara ◽  
Gustavo R. Daleo ◽  
Claudia R. Oliva
Keyword(s):  
Aspartic Protease ◽  
Purification And Characterization

Purification and characterization of a milk-clotting aspartic protease from Withania coagulans fruit

2017 ◽  
Vol 98 ◽  
pp. 847-854 ◽  
Author(s):  
Mahmoud Salehi ◽  
Mahmoud Reza Aghamaali ◽  
Reza H. Sajedi ◽  
S. Mohsen Asghari ◽  
Eisa Jorjani
Keyword(s):  
Aspartic Protease ◽  
Purification And Characterization ◽  
Milk Clotting ◽  
Withania Coagulans

scholarly journals Purification and characterization of an aspartic protease from the Rhizopus oryzae protease extract, Peptidase R

2014 ◽  
Vol 17 (2) ◽  
pp. 89-94 ◽  
Author(s):  
Nai-Wan Hsiao ◽  
Yeh Chen ◽  
Yi-Chia Kuan ◽  
Yen-Chung Lee ◽  
Shuo-Kang Lee ◽  
...  
Keyword(s):  
Rhizopus Oryzae ◽  
Aspartic Protease ◽  
Purification And Characterization

Purification and characterization of a novel aspartic protease from basidiomycetous yeast Cryptococcus sp. S-2

2011 ◽  
Vol 112 (5) ◽  
pp. 441-446 ◽  
Author(s):  
Shengbin Rao ◽  
Osamu Mizutani ◽  
Takuya Hirano ◽  
Kazuo Masaki ◽  
Haruyuki Iefuji
Keyword(s):  
Aspartic Protease ◽  
Basidiomycetous Yeast ◽  
Purification And Characterization

Purification and Characterization of Aspartic Protease Produced from Aspergillus oryzae DRDFS13 MN726447 under Solid-State Fermentation

2021 ◽  
Author(s):  
Jermen Mamo ◽  
Martin Kangwa ◽  
Jorge Fernando Suarez Orellana ◽  
Vikas Yelemane ◽  
Hector Marcelo Fernandez-Lahore ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Oryzae ◽  
Solid State Fermentation ◽  
Aspartic Protease ◽  
Purification And Characterization
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