Polypeptides IGF-1C and P24 synergistically promote osteogenic differentiation of bone marrow mesenchymal stem cells in vitro through the p38 and JNK signaling pathways

Background: In vitro impact of dihydrotestosterone (DHT) and 17-estradiol (E2) in osteogenic differentiation of castrated rat bone marrow mesenchymal stem cells (rBMMSC) still need to be clarified. Materials and Methods: The viability, proliferation and density of cultured rBMMSC isolated from sham operated (Sham) and castrated (Cast) male rats were evaluated. rBMMSC were cultured with osteogenic differentiating medium (ODM) in the presence of DHT (5,10 nM) and E2 (10,100 nM). Osteogenesis was evaluated by alizarin red staining and measurement of calcium deposition and bone alkaline phosphatase (BALP) activity. Results: Population doubling (PD) of rBMMSC isolated from Cast rats was significantly lower (P<0.05) compared to that isolated from Sham rats. rBMMSC from Cast rats showed low scattered calcified nodule after culturing in ODM and did not cause a significant increase in calcium deposition and B-ALP activity compared to rBMMSCs from Sham rats. Exposure of rBMMSC isolated from Cast rats to DHT (5 nM) or E2 (10 nM) in ODM showed medium scattered calcified nodules with significantly higher (P<0.05) calcium deposition and B-ALP activity. Moreover, exposure of rBMMSC to DHT (10 nM) or E2 (100 nM) showed high scattered calcified nodules with higher (P<0.01) calcium deposition and B-ALP activity Conclusion: These results indicated that the presence of testes might participate in controlling the in vitro proliferation and osteogenic differentiation capacity of rBMMSCs. DHT and E2 can enhance the osteogenic capacity of rBMMSCs in a dose-dependent manner. Based on these observations, optimum usage of DHT and E2 can overcome the limitations of MSCs and advance the therapeutic bone regeneration potential in the future.

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Withanolide B promotes osteogenic differentiation of human bone marrow mesenchymal stem cells via ERK1/2 and Wnt/β-catenin signaling pathways

International Immunopharmacology ◽

10.1016/j.intimp.2020.106960 ◽

2020 ◽

Vol 88 ◽

pp. 106960

Author(s):

Zhihui Kuang ◽

Jinwu Bai ◽

Licheng Ni ◽

Kai Hang ◽

Jianxiang Xu ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Osteogenic Differentiation ◽

Signaling Pathways ◽

Human Bone ◽

Human Bone Marrow ◽

Marrow Mesenchymal Stem Cells

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In vitro facilitating role of polygonatum sibiricum polysaccharide in osteogenic differentiation of bone marrow mesenchymal stem cells from patients with multiple myeloma

Biotechnology Letters ◽

10.1007/s10529-021-03125-x ◽

2021 ◽

Author(s):

Jianqiang Zhao ◽

Lijie Ma ◽

Zengfeng Ni ◽

Hui Liu

Keyword(s):

Stem Cells ◽

Multiple Myeloma ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Osteogenic Differentiation ◽

Marrow Mesenchymal Stem Cells

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Electrospun poly-l-lactic acid nanofibers decorated with melt-derived S53P4 bioactive glass nanoparticles: The effect of nanoparticles on proliferation and osteogenic differentiation of human bone marrow mesenchymal stem cells in vitro

Ceramics International ◽

10.1016/j.ceramint.2018.08.005 ◽

2018 ◽

Vol 44 (16) ◽

pp. 20211-20219 ◽

Cited By ~ 6

Author(s):

Mehdi Shams ◽

Mohammad Karimi ◽

Marzieh Ghollasi ◽

Nader Nezafati ◽

Ali Salimi

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Lactic Acid ◽

Bioactive Glass ◽

Osteogenic Differentiation ◽

Human Bone ◽

Human Bone Marrow ◽

Marrow Mesenchymal Stem Cells

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Expression of Pcp4 gene during osteogenic differentiation of bone marrow mesenchymal stem cells in vitro

Molecular and Cellular Biochemistry ◽

10.1007/s11010-007-9652-x ◽

2007 ◽

Vol 309 (1-2) ◽

pp. 143-150 ◽

Cited By ~ 11

Author(s):

Jingang Xiao ◽

Yao Wu ◽

Runliang Chen ◽

Yunfeng Lin ◽

Ling Wu ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Osteogenic Differentiation ◽

Marrow Mesenchymal Stem Cells

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Long non-coding RNA MIR22HG promotes osteogenic differentiation of bone marrow mesenchymal stem cells via PTEN/ AKT pathway

Cell Death and Disease ◽

10.1038/s41419-020-02813-2 ◽

2020 ◽

Vol 11 (7) ◽

Author(s):

Chanyuan Jin ◽

Lingfei Jia ◽

Zhihui Tang ◽

Yunfei Zheng

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Osteogenic Differentiation ◽

Mineral Density ◽

Tensin Homolog ◽

Human Bmscs ◽

Marrow Mesenchymal Stem Cells

Abstract Osteoporosis is a prevalent metabolic bone disease characterized by low bone mineral density and degenerative disorders of bone tissues. Previous studies showed the abnormal osteogenic differentiation of endogenous bone marrow mesenchymal stem cells (BMSCs) contributes to the development of osteoporosis. However, the underlying mechanisms by which BMSCs undergo osteogenic differentiation remain largely unexplored. Recently, long non-coding RNAs have been discovered to play important roles in regulating BMSC osteogenesis. In this study, we first showed MIR22HG, which has been demonstrated to be involved in the progression of several cancer types, played an important role in regulating BMSC osteogenesis. We found the expression of MIR22HG was significantly decreased in mouse BMSCs from the osteoporotic mice and it was upregulated during the osteogenic differentiation of human BMSCs. Overexpression of MIR22HG in human BMSCs enhanced osteogenic differentiation, whereas MIR22HG knockdown inhibited osteogenic differentiation both in vitro and in vivo. Mechanistically, MIR22HG promoted osteogenic differentiation by downregulating phosphatase and tensin homolog (PTEN) and therefore activating AKT signaling. Moreover, we found MIR22HG overexpression promoted osteoclastogenesis of RAW264.7 cells, which indicated that MIR22HG played a significant role in bone metabolism and could be a therapeutic target for osteoporosis and other bone-related diseases.

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