scholarly journals Disintegrin-like/cysteine-rich domains of the reprolysin HF3: Site-directed mutagenesis reveals essential role of specific residues

Biochimie ◽  
2011 ◽  
Vol 93 (2) ◽  
pp. 345-351 ◽  
Author(s):  
Milene C. Menezes ◽  
Ana Karina de Oliveira ◽  
Robson L. Melo ◽  
Mônica Lopes-Ferreira ◽  
Vanessa Rioli ◽  
...  
Keyword(s):  
Essential Role ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

scholarly journals Experimental evidence for the essential role of the C-terminal residue in the strict aminopeptidase activity of the thiol aminopeptidase PepC, a bacterial bleomycin hydrolase

1997 ◽  
Vol 328 (2) ◽  
pp. 343-347 ◽  
Author(s):  
Luis MATA ◽  
Marta ERRA-PUJADA ◽  
Jean-Claude GRIPON ◽  
Michel-Yves MISTOU
Keyword(s):  
Essential Role ◽  
Site Directed Mutagenesis ◽  
Structural Basis ◽  
Aminopeptidase Activity ◽  
Directed Mutagenesis ◽  
Wild Type ◽  
Terminal Residue ◽  
Bleomycin Hydrolase ◽  
Eukaryotic Organisms

PepCs isolated from lactic acid bacteria and bleomycin hydrolases of eukaryotic organisms are strict aminopeptidases which belong to the papain family of thiol peptidases. The structural basis of the enzymic specificity of the lactococcal PepC has been investigated by site-directed mutagenesis. The deletion of the C-terminal residue (Ala-435) abolished the aminopeptidase activity, whereas this deletion led to a new peptidase specificity. The enzymic properties of wild-type and mutant PepCs demonstrate that the terminal α-carboxy group plays a key role in the strict aminopeptidase activity.

scholarly journals Site-directed mutagenesis of tyrosine hydroxylase. Role of serine 40 in catalysis.

1992 ◽  
Vol 267 (36) ◽  
pp. 25754-25758
Author(s):  
J Wu ◽  
D Filer ◽  
A.J. Friedhoff ◽  
M Goldstein
Keyword(s):  
Tyrosine Hydroxylase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

scholarly journals The role of Val68(E11) in ligand binding to sperm whale myoglobin. Site-directed mutagenesis of a synthetic gene.

1990 ◽  
Vol 265 (20) ◽  
pp. 11788-11795
Author(s):  
K D Egeberg ◽  
B A Springer ◽  
S G Sligar ◽  
T E Carver ◽  
R J Rohlfs ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Sperm Whale ◽  
Site Directed Mutagenesis ◽  
Synthetic Gene ◽  
Directed Mutagenesis ◽  
Sperm Whale Myoglobin

Probing the Role of Tyr 64 ofTreponema denticolaCystalysin by Site-Directed Mutagenesis and Kinetic Studies†

Biochemistry ◽  
2005 ◽  
Vol 44 (42) ◽  
pp. 13970-13980 ◽  
Author(s):  
Barbara Cellini ◽  
Mariarita Bertoldi ◽  
Riccardo Montioli ◽  
Carla Borri Voltattorni
Keyword(s):  
Kinetic Studies ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis
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