Electrochemical DNA hybridization detection using peptide nucleic acids and [Ru(NH3)6]3+ on gold electrodes

2007 ◽  
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pp. 3544-3549 ◽  
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Tirayut Vilaivan ◽  
Wanwimon Mekboonsonglarp ◽  
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Vol 233 ◽  
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Fanny Langschwager ◽  
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Gerd-Uwe Flechsig

2002 ◽  
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J. W. Park ◽  
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Hiro. Tanaka ◽  
...  

Our system is the electrochemical approaches include the detection of hybridization from nonlabeling nucleic acids to protein-bound nucleic acids using soluble mediators with K4Fe(CN)6 solutions. In order to generate bio-functional surfaces, the streptavidin(SAv)-biotin system is used. A 50 % change of redox peak current after hybridization measured with 50 μM concentration of target DNA. We suggest that this result comes from the efficient electron transport through the SAv-biotin interaction. Our electrochemical detection system showed good reproducibility on a chip with non-labeling DNA hybridization detection.


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