Amperometric glucose biosensor based on multilayer films via layer-by-layer self-assembly of multi-wall carbon nanotubes, gold nanoparticles and glucose oxidase on the Pt electrode

The study reports a facile and eco-friendly approach for nanomaterial synthesis and enzyme immobilization. A corresponding glucose biosensor was fabricated by immobilizing the gold nanoparticles (AuNPs) and glucose oxidase (GOD) multilayer films onto the polypyrrole (PPy)/reduced graphene oxide (RGO) modified glassy carbon electrode (GCE) via the electrodeposition and self-assembly. PPy and graphene oxide were first coated on the surface of a bare GCE by the electrodeposition. Then, AuNPs and GOD were alternately immobilized onto PPy-RGO/GCE electrode using the electrodeposition of AuNPs and self-assembly of GOD to obtain AuNPs-GOD multilayer films. The resulting PPy-RGO-(AuNPs-GOD)n/GCE biosensors were used to characterize and assess their electrocatalytic activity toward glucose using cyclic voltammetry and amperometry. The response current increased with the increased number of AuNPs-GOD layers, and the biosensor based on four layers of AuNPs-GOD showed the best performance. The PPy-RGO-(AuNPs-GOD)4/GCE electrode can detect glucose in a linear range from 0.2 mM to 8 mM with a good sensitivity of 0.89 μA/mM, and a detection limit of 5.6 μM (S/N = 3). This study presents a promising eco-friendly biosensor platform with advantages of electrodeposition and self-assembly, and would be helpful for the future design of more complex electrochemical detection systems.

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Layer-by-layer assemblies of chitosan/multi-wall carbon nanotubes and glucose oxidase for amperometric glucose biosensor applications

Materials Science and Engineering C ◽

10.1016/j.msec.2008.07.018 ◽

2009 ◽

Vol 29 (1) ◽

pp. 346-349 ◽

Cited By ~ 44

Author(s):

Bao-Yan Wu ◽

Shi-Hua Hou ◽

Min Yu ◽

Xia Qin ◽

Sha Li ◽

...

Keyword(s):

Carbon Nanotubes ◽

Glucose Oxidase ◽

Glucose Biosensor ◽

Layer By Layer ◽

Multi Wall Carbon Nanotubes ◽

Biosensor Applications

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Transparent and flexible glucose biosensor via layer-by-layer assembly of multi-wall carbon nanotubes and glucose oxidase

Electrochemistry Communications ◽

10.1016/j.elecom.2006.12.022 ◽

2007 ◽

Vol 9 (6) ◽

pp. 1269-1275 ◽

Cited By ~ 121

Author(s):

X.B. Yan ◽

X.J. Chen ◽

B.K. Tay ◽

K.A. Khor

Keyword(s):

Carbon Nanotubes ◽

Glucose Oxidase ◽

Glucose Biosensor ◽

Layer By Layer ◽

Multi Wall Carbon Nanotubes ◽

Layer By Layer Assembly ◽

Layer Assembly

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DNA Biosensors Based on Layer-by-Layer Self-Assembled Multilayer Films of Carbon Nanotubes and Gold Nanoparticles

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.298.84 ◽

2011 ◽

Vol 298 ◽

pp. 84-84 ◽

Cited By ~ 1

Keyword(s):

Carbon Nanotubes ◽

Gold Nanoparticles ◽

Multilayer Films ◽

Layer By Layer ◽

Dna Biosensors ◽

Self Assembled ◽

Double Publication

The paper has been withdrawn do to double publication. The original was published by SPIE Proceedings of SPIE, vol 7493, 74935A in 2009 (DOI:10.1117/12.840316)

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