Using the Area of Contact between Layers as a Predictor for the Anisotropic Strength of Parts Produced by FDM

Fused Filament Fabrication (FFF), better known as FDM© (Fused Deposition Modeling) is an additive manufacturing process (AM) by which a physical object can be created from a 3D model generated in the computer, through layer-by-layer deposition of semi-melted plastic filaments. However, parts produced by the FDM process have different characteristics compared to parts produced by traditional methods such as plastic injection, especially with regard to mechanical properties related to stresses (tensile, compression, torsion and shear), due to the anisotropic nature of the process deposition. Many works have been carried out in order to determine the influence between the FDM process parameters and the mechanical characteristics of parts produced by this technology. Traditionally, the studied parameters comprise those that are adjusted in slicing software, which does not satisfactorily reflect the bond between the layers. This work uses the area of contact between the layers as the determining factor of the transverse tensile strength to bedding and suggests a methodology for the determination of this parameter. Using analysis of variance (ANOVA) and the Taguchi analysis method, we identified the contact area between the layers as the most relevant parameter for tensile strength in the transverse direction of the printed layers with a relevance of more than 95% over the others investigated parameters. From the survey of relevant properties, new tests were carried out to determine a mathematical model to predict the minimum slicing parameters that should be used to obtain the required strength. Keywords: Fused Deposition Modeling, Mechanical Strength, AM Anisotropic Property, Layer Bond Properties, PLA.

Poly-L-Lysine and Human Plasmatic Fibronectin Films as Proactive Coatings to Improve Implant Biointegration

The success of stable and long-term implant integration implies the promotion, control, and respect of the cell microenvironment at the site of implantation. The key is to enhance the implant–host tissue cross talk by developing interfacial strategies that guarantee an optimal and stable seal of soft tissue onto the implant, while preventing potential early and late infection. Indeed, implant rejection is often jeopardized by lack of stable tissue surrounding the biomaterial combined with infections which reduce the lifespan and increase the failure rate of implants and morbidity and account for high medical costs. Thin films formed by the layer-by-layer (LbL) assembly of oppositely charged polyelectrolytes are particularly versatile and attractive for applications involving cell–material contact. With the combination of the extracellular matrix protein fibronectin (Fn, purified from human plasma) and poly-L-lysine (PLL, exhibiting specific chain lengths), we proposed proactive and biomimetic coatings able to guarantee enhanced cell attachment and exhibiting antimicrobial properties. Fn, able to create a biomimetic interface that could enhance cell attachment and promote extracellular cell matrix remodeling, is incorporated as the anionic polymer during film construction by the LbL technic whereas PLL is used as the cationic polymer for its capacity to confer remarkable antibacterial properties.