Single particle detection of protein molecules using dark-field microscopy to avoid signals from nonspecific adsorption

2020 ◽  
Vol 169 ◽  
pp. 112612
Author(s):  
Bijan P. Markhali ◽  
Manish Sriram ◽  
Danielle T. Bennett ◽  
Parisa S. Khiabani ◽  
Sharmin Hoque ◽  
...  
Keyword(s):  
Single Particle ◽  
Dark Field ◽  
Particle Detection ◽  
Nonspecific Adsorption ◽  
Dark Field Microscopy ◽  
Protein Molecules ◽  
Single Particle Detection

Optical single-particle detection in nanoholes towards simple parallel detection of molecular binding events

2012 ◽  
Vol 93 (2) ◽  
pp. 140-151 ◽  
Author(s):  
Norbert Jahr ◽  
Nicole Hädrich ◽  
Mamuna Anwar ◽  
Andrea Csaki ◽  
Ondra Stranik ◽  
...  
Keyword(s):  
Single Particle ◽  
Particle Detection ◽  
Molecular Binding ◽  
Parallel Detection ◽  
Single Particle Detection

scholarly journals Development, Characterization, and Application of a Versatile Single Particle Detection Apparatus for Time-Integrated and Time-Resolved Fluorescence Measurements—Part II: Experimental Evaluation

2009 ◽  
Vol 2009 ◽  
pp. 1-14
Author(s):  
Xihong Wu ◽  
J. A. Merten ◽  
N. Omenetto ◽  
B. W. Smith ◽  
J. D. Winefordner
Keyword(s):  
Single Particle ◽  
Narrow Beam ◽  
Time Resolved Fluorescence ◽  
Particle Detection ◽  
Time Resolved ◽  
Endogenous Fluorophores ◽  
Fluorescence Measurements ◽  
On Line ◽  
Single Particle Detection ◽  
Speed Mode

This paper describes the experimental realization and characterization of a versatile single particle detection apparatus. The system utilizes a novel particle beam inlet that can serve as either an on-line particle concentrator (i.e., all diameters confined in a narrow beam) or as a segregator (i.e., selected diameters confined in a narrow beam) and can be operated in a high-speed mode as well as in a low-speed mode, thus allowing different interaction times between the particles and the laser beam. An aerodynamic sizing technique has been incorporated into the system to provide rapid, real-time, and high-resolution sizing. Parameters such as transmission efficiency and size-segregation efficiency have been measured. The performance of the instrument has been demonstrated by on-line detection of spectrally resolved and time resolved fluorescence detection from airborne dye-doped particles and aerosolized endogenous fluorophores found in biological agents.

scholarly journals Modelling of Evanescent Field Scattering

Proceedings ◽  
2020 ◽  
Vol 56 (1) ◽  
pp. 16
Author(s):  
Andreas Tortschanoff ◽  
Marcus Baumgart ◽  
Jaka Pribošek
Keyword(s):  
Numerical Analysis ◽  
Single Particle ◽  
Evanescent Field ◽  
Promising Method ◽  
Analytical Models ◽  
Particle Scattering ◽  
Particle Detection ◽  
Sensing Mechanism ◽  
Single Particle Detection

Evanescent field particle scattering is a promising method for single particle detection. In this study, we performed a detailed numerical analysis to show the possibilities and limitations of analytical models for predicting the capabilities of this sensing mechanism.

scholarly journals A nanochannel through a plasmonic antenna gap: an integrated device for single particle counting

Lab on a Chip ◽  
2019 ◽  
Vol 19 (14) ◽  
pp. 2394-2403 ◽  
Author(s):  
Irene Fernandez-Cuesta ◽  
Melanie Maputol West ◽  
Enrica Montinaro ◽  
Adam Schwartzberg ◽  
Stefano Cabrini
Keyword(s):  
Real Time ◽  
Single Particle ◽  
Particle Detection ◽  
Particle Counting ◽  
Detection And Counting ◽  
Single Particle Detection ◽  
Integrated Device

A gold bowtie nanoantenna integrated with a 30 nm × 30 nm nanochannel for single particle detection and counting in real time.

Dual-Height Fluidic Channels for Particle Concentration Integrated with Micropore Sensors for Single Particle Detection

2017 ◽  
Vol 2017 (0) ◽  
pp. J0270203
Author(s):  
Wataru TONOMURA ◽  
Makusu TSUTSUI ◽  
Kazumichi YOKOTA ◽  
Akihide ARIMA ◽  
Masateru TANIGUCHI ◽  
...  
Keyword(s):  
Single Particle ◽  
Particle Concentration ◽  
Particle Detection ◽  
Single Particle Detection
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