Synthesis of 2′-O-alkylcarbamoylethyl-modified oligonucleotides with enhanced nuclease resistance that form isostable duplexes with complementary RNA

2021 ◽  
Vol 35 ◽  
pp. 127779
Author(s):  
Tomohiro Kishimura ◽  
Takahito Tomori ◽  
Yoshiaki Masaki ◽  
Kohji Seio
Keyword(s):  
Modified Oligonucleotides ◽  
Nuclease Resistance

Synthesis and properties of novel 2′-C,4′-C-ethyleneoxy-bridged 2′-deoxyribonucleic acids with exocyclic methylene groups

2016 ◽  
Vol 14 (40) ◽  
pp. 9481-9484 ◽  
Author(s):  
Takashi Osawa ◽  
Satoshi Obika ◽  
Yoshiyuki Hari
Keyword(s):  
Binding Affinity ◽  
Modified Oligonucleotides ◽  
Strong Binding ◽  
Nuclease Resistance ◽  
Methylene Groups ◽  
Exocyclic Methylene

Three methylene-EoDNAs were synthesized from 5-methyluridine and their modified oligonucleotides showed strong binding affinity with ssRNA and high nuclease resistance.

scholarly journals Synthesis and Antisense Properties of 2′β-F-Arabinouridine Modified Oligonucleotides with 4′-C-OMe Substituent

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2374 ◽  
Author(s):  
Xiao-Yang He ◽  
Jing Wang ◽  
Dan-Dan Lu ◽  
Sheng-Qi Wang
Keyword(s):  
Chemical Modification ◽  
Binding Affinity ◽  
Therapeutic Strategy ◽  
Modified Oligonucleotides ◽  
Nuclease Resistance ◽  
Modified Oligonucleotide

A novel 2′-F,4′-C-OMe–arabinouridine (araU) was successfully synthesized and introduced into oligonucleotides. The oligonucleotide containing 2′-F,4′-C-OMe–araU exhibited improved nuclease resistance and RNA hybridizing selective ability relative to 2′-F–araU. In particular, when 2′-F,4′-C-OMe–araU inserted into C–H⋯F–C bonding-favorable 5′–uridine–purine–3′ steps, the modified oligonucleotide showed remarkable binding affinity and selectivity to RNA complements. Thus, 2′-F,4′-C-OMe–araU has valuable antisense properties and can be used as novel chemical modification for antisense therapeutic strategy.

ChemInform Abstract: Synthesis, Hybridization, and Nuclease Resistance Properties of 2′-O-Aminooxyethyl Modified Oligonucleotides

ChemInform ◽  
2010 ◽  
Vol 30 (44) ◽  
pp. no-no
Author(s):  
Andrew M. Kawasaki ◽  
Martin D. Casper ◽  
Thazha P. Prakash ◽  
Sheri Manalili ◽  
Henri Sasmor ◽  
...  
Keyword(s):  
Modified Oligonucleotides ◽  
Nuclease Resistance

Synthesis, Hybridization, and Nuclease Resistance Properties of 2′-O-Aminooxyethyl Modified Oligonucleotides

1999 ◽  
Vol 18 (6-7) ◽  
pp. 1419-1420 ◽  
Author(s):  
Andrew M. Kawasaki ◽  
Martin D. Casper ◽  
Thazha P. Prakash ◽  
Sheri Manalili ◽  
Henri Sasmor ◽  
...  
Keyword(s):  
Modified Oligonucleotides ◽  
Nuclease Resistance

scholarly journals Nuclease Resistance and Antisense Activity of Modified Oligonucleotides Targeted to Ha-ras

1996 ◽  
Vol 271 (24) ◽  
pp. 14533-14540 ◽  
Author(s):  
Brett P. Monia ◽  
Joseph F. Johnston ◽  
Henri Sasmor ◽  
Lendell L. Cummins
Keyword(s):  
Modified Oligonucleotides ◽  
Nuclease Resistance

Synthesis, hybridization, and nuclease resistance properties of 2′-O-aminooxyethyl (2′-O-AOE) modified oligonucleotides

1999 ◽  
Vol 40 (4) ◽  
pp. 661-664 ◽  
Author(s):  
Andrew M. Kawasaki ◽  
Martin D. Casper ◽  
Thazha P. Prakash ◽  
Sheri Manalili ◽  
Henri Sasmor ◽  
...  
Keyword(s):  
Modified Oligonucleotides ◽  
Nuclease Resistance

Synthesis, binding, nuclease resistance and cellular uptake properties of 2′- O -acetalester-modified oligonucleotides containing cationic groups

2015 ◽  
Vol 23 (17) ◽  
pp. 5360-5368 ◽  
Author(s):  
Annabelle Biscans ◽  
Sonia Rouanet ◽  
Jean-Rémi Bertrand ◽  
Jean-Jacques Vasseur ◽  
Christelle Dupouy ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Modified Oligonucleotides ◽  
Nuclease Resistance

Sugar modified oligonucleotides. III (1). Synthesis, nuclease resistance and base pairing properties of α- and β-L-octathymidylates

1990 ◽  
Vol 172 (2) ◽  
pp. 537-543 ◽  
Author(s):  
François Morvan ◽  
Corinne Génu ◽  
Bernard Rayner ◽  
Gilles Gosselin ◽  
Jean-Louis Imbach
Keyword(s):  
Modified Oligonucleotides ◽  
Base Pairing ◽  
Nuclease Resistance

scholarly journals Structural Basis of Duplex Thermodynamic Stability and Enhanced Nuclease Resistance of 5′-C-Methyl Pyrimidine-Modified Oligonucleotides

2016 ◽  
Vol 81 (6) ◽  
pp. 2261-2279 ◽  
Author(s):  
Alexander V. Kel′in ◽  
Ivan Zlatev ◽  
Joel Harp ◽  
Muthusamy Jayaraman ◽  
Anna Bisbe ◽  
...  
Keyword(s):  
Thermodynamic Stability ◽  
Structural Basis ◽  
Modified Oligonucleotides ◽  
Nuclease Resistance
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