Molecular characterization of a phospholipid-hydroperoxide glutathione peroxidase from the bumblebee Bombus ignitus

2010 ◽  
Vol 155 (1) ◽  
pp. 54-61 ◽  
Author(s):  
Zhigang Hu ◽  
Kwang Sik Lee ◽  
Young Moo Choo ◽  
Hyung Joo Yoon ◽  
Iksoo Kim ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Molecular Characterization ◽  
Phospholipid Hydroperoxide Glutathione Peroxidase ◽  
Phospholipid Hydroperoxide ◽  
Bombus Ignitus

Expression and characterization of a phospholipid hydroperoxide glutathione peroxidase gene inSchistosoma japonicum

Parasitology ◽  
2015 ◽  
Vol 142 (13) ◽  
pp. 1595-1604 ◽  
Author(s):  
YING ZHANG ◽  
YUAN HE ◽  
LI HE ◽  
HONGYING ZONG ◽  
GUOBIN CAI
Keyword(s):  
Glutathione Peroxidase ◽  
Egg Production ◽  
Transcript Level ◽  
Structure Characteristic ◽  
Lipid Hydroperoxides ◽  
Phospholipid Hydroperoxide Glutathione Peroxidase ◽  
Reverse Transcription Pcr ◽  
Phospholipid Hydroperoxide ◽  
Interaction Domains

SUMMARYPhospholipid hydroperoxide glutathione peroxidase (PHGPx, GPx4) is a major antioxidant enzyme, which plays unique roles in the protection of cells against oxidative stress by catalysing reduction of lipid hydroperoxides. We isolated and characterized a full-length cDNA sequence encodingGPxgene from a blood fluke,Schistosoma japonicum(designatedSjGPx), which contained an in-frame TGA codon for selenocysteine (Sec) and a concurrent Sec insertion sequence in its 3′-untranslated region. Protein encoded bySjGPxdemonstrated a primary structure characteristic to the PHGPx family, including preservation of catalytic domains and absence of the subunit interaction domains. Semi-quantitative reverse transcription PCR and Western blotting showed that the SjGPx was mainly expressed in the female adults and eggs. RNA interference approach was employed to investigate the effects of knockdown ofSjGPx. SjGPx expression level was significantly reduced on the 5th day post-RNAi. Significantly reduction in GPx enzyme activities, as well as obvious changes in morphology of intrauterine eggs followed the reduction inSjGPxtranscript level. We observed a 63·04% reduction in GPx activity and the eggs severely deformed. Our results revealed that SjGPx protein might be involved in the provision of enzyme activity during egg production.

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