scholarly journals A Role for BLM in Double-Strand Break Repair Pathway Choice: Prevention of CtIP/Mre11-Mediated Alternative Nonhomologous End-Joining

Cell Reports ◽  
2013 ◽  
Vol 5 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Anastazja Grabarz ◽  
Josée Guirouilh-Barbat ◽  
Aurélia Barascu ◽  
Gaëlle Pennarun ◽  
Diane Genet ◽  
...  
Keyword(s):  
Strand Break ◽  
Double Strand Break ◽  
Nonhomologous End Joining ◽  
Double Strand Break Repair ◽  
Repair Pathway ◽  
End Joining ◽  
Pathway Choice ◽  
Break Repair

Analysis of DNA Double-Strand Break Repair by Nonhomologous End Joining in Cell-Free Extracts From Mammalian Cells

2004 ◽  
pp. 351-372 ◽  
Author(s):  
Petra Pfeiffer ◽  
Elke Feldmann ◽  
Andrea Odersky ◽  
Steffi Kuhfittig-Kulle ◽  
Wolfgang Goedecke
Keyword(s):  
Mammalian Cells ◽  
Strand Break ◽  
Double Strand Break ◽  
Nonhomologous End Joining ◽  
Double Strand Break Repair ◽  
End Joining ◽  
Dna Double Strand Break ◽  
Break Repair

scholarly journals A histone H3K36 chromatin switch coordinates DNA double-strand break repair pathway choice

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Chen-Chun Pai ◽  
Rachel S. Deegan ◽  
Lakxmi Subramanian ◽  
Csenge Gal ◽  
Sovan Sarkar ◽  
...  
Keyword(s):  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Repair Pathway ◽  
Dna Double Strand Break ◽  
Pathway Choice ◽  
Break Repair

scholarly journals SHLD 2/ FAM 35A co‐operates with REV 7 to coordinate DNA double‐strand break repair pathway choice

2018 ◽  
Vol 37 (18) ◽  
Author(s):  
Steven Findlay ◽  
John Heath ◽  
Vincent M Luo ◽  
Abba Malina ◽  
Théo Morin ◽  
...  
Keyword(s):  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Repair Pathway ◽  
Dna Double Strand Break ◽  
Pathway Choice ◽  
Break Repair

scholarly journals Role of 53BP1 in the Regulation of DNA Double-Strand Break Repair Pathway Choice

2014 ◽  
Vol 181 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Arun Gupta ◽  
Clayton R. Hunt ◽  
Sharmistha Chakraborty ◽  
Raj K. Pandita ◽  
John Yordy ◽  
...  
Keyword(s):  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Repair Pathway ◽  
Dna Double Strand Break ◽  
Pathway Choice ◽  
Break Repair

scholarly journals Polymerase Mu Is a DNA-Directed DNA/RNA Polymerase

2003 ◽  
Vol 23 (7) ◽  
pp. 2309-2315 ◽  
Author(s):  
Stephanie A. Nick McElhinny ◽  
Dale A. Ramsden
Keyword(s):  
High Specificity ◽  
Strand Break ◽  
Double Strand Break ◽  
Nonhomologous End Joining ◽  
Double Strand Break Repair ◽  
End Joining ◽  
Strand Breaks ◽  
Break Repair ◽  
The Impact

ABSTRACT DNA polymerases are defined as such because they use deoxynucleotides instead of ribonucleotides with high specificity. We show here that polymerase mu (pol μ), implicated in the nonhomologous end-joining pathway for repair of DNA double-strand breaks, incorporates both ribonucleotides and deoxynucleotides in a template-directed manner. pol μ has an approximately 1,000-fold-reduced ability to discriminate against ribonucleotides compared to that of the related pol β, although pol μ's substrate specificity is similar to that of pol β in most other respects. Moreover, pol μ more frequently incorporates ribonucleotides when presented with nucleotide concentrations that approximate cellular pools. We therefore addressed the impact of ribonucleotide incorporation on the activities of factors required for double-strand break repair by nonhomologous end joining. We determined that the ligase required for this pathway readily joined strand breaks with terminal ribonucleotides. Most significantly, pol μ frequently introduced ribonucleotides into the repair junctions of an in vitro nonhomologous end-joining reaction, an activity that would be expected to have important consequences in the context of cellular double-strand break repair.

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