Effect of chromatographic conditions on resolution in high-performance ion-exchange chromatography of proteins on macroporous anion-exchange resin

2004 ◽  
Vol 1031 (1-2) ◽  
pp. 101-105 ◽  
Author(s):  
Yoshio Kato ◽  
Koji Nakamura ◽  
Takashi Kitamura ◽  
Teruhiko Tsuda ◽  
Masazumi Hasegawa ◽  
...  
1988 ◽  
Vol 450 (3) ◽  
pp. 343-352 ◽  
Author(s):  
Takao Oi ◽  
Tetsuro Tsukamoto ◽  
Hideki Akai ◽  
Hidetake Kakihana ◽  
Morikazu Hosoe

1980 ◽  
Vol 26 (2) ◽  
pp. 345-347 ◽  
Author(s):  
G P James ◽  
M H DJang ◽  
H H Hamilton

Abstract When a series of patients' urine samples supplemented in vitro with chlorpromazine or imipramine was assayed with the Forrest qualitative assays, we observed an occasional false-negative result, which we found was attributable to interference by ascorbic acid. It interferes with the reagent, not with the analytes, in both assays. We easily eliminated this interference with the phenothiazine test by using an anion-exchange resin. Eliminating the interference with the assay for imipramine, however, is more difficult; false-negative results can be obtained even after ion-exchange chromatography if the imipramine concentration is less than 50 mg/L.


1980 ◽  
Vol 26 (2) ◽  
pp. 345-347
Author(s):  
G P James ◽  
M H DJang ◽  
H H Hamilton

Abstract When a series of patients' urine samples supplemented in vitro with chlorpromazine or imipramine was assayed with the Forrest qualitative assays, we observed an occasional false-negative result, which we found was attributable to interference by ascorbic acid. It interferes with the reagent, not with the analytes, in both assays. We easily eliminated this interference with the phenothiazine test by using an anion-exchange resin. Eliminating the interference with the assay for imipramine, however, is more difficult; false-negative results can be obtained even after ion-exchange chromatography if the imipramine concentration is less than 50 mg/L.


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