Simultaneous separation of acidic and basic proteins using gemini pyrrolidinium surfactants and hexafluoroisopropanol as dynamic coating additives in capillary electrophoresis

2015 ◽  
Vol 1412 ◽  
pp. 151-158 ◽  
Author(s):  
Yu Tian ◽  
Yunfang Li ◽  
Jie Mei ◽  
Bo Cai ◽  
Jinfeng Dong ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Dynamic Coating ◽  
Basic Proteins ◽  
Simultaneous Separation

Polymeric ionic liquid as a dynamic coating additive for separation of basic proteins by capillary electrophoresis

2010 ◽  
Vol 674 (2) ◽  
pp. 243-248 ◽  
Author(s):  
Jing Li ◽  
Haifeng Han ◽  
Qing Wang ◽  
Xia Liu ◽  
Shengxiang Jiang
Keyword(s):  
Ionic Liquid ◽  
Capillary Electrophoresis ◽  
Dynamic Coating ◽  
Polymeric Ionic Liquid ◽  
Basic Proteins

Separation of Acidic and Basic Proteins by Nanoparticle-Filled Capillary Electrophoresis

2006 ◽  
Vol 78 (23) ◽  
pp. 8004-8010 ◽  
Author(s):  
Cheng-Ju Yu ◽  
Chih-Lin Su ◽  
Wei-Lung Tseng
Keyword(s):  
Capillary Electrophoresis ◽  
Basic Proteins

Simultaneous Separation and Determination of Vanillin and O-Vanillin by CE Compared with HPLC

2011 ◽  
Vol 236-238 ◽  
pp. 2725-2728 ◽  
Author(s):  
Jin Guan ◽  
Feng Yan ◽  
Shuang Shi ◽  
Yun Li
Keyword(s):  
High Performance Liquid Chromatography ◽  
Capillary Electrophoresis ◽  
Liquid Chromatography ◽  
Applied Voltage ◽  
High Performance ◽  
Hydrogen Phosphate ◽  
Disodium Hydrogen Phosphate ◽  
Real Samples ◽  
Simultaneous Separation

A method for the simultaneous separation and determination of vanillin ando-vanillin by capillary electrophoresis (CE) was developed. The influence of type, concentration and pH of running buffer, and applied voltage on separation was investigated. Under the condition of 50 mmol/L borax-150 mmol/L disodium hydrogen phosphate (pH 7.5) and applied voltage of 15 kV, the vanillin ando-vanillin were separated in less than 6 min. The method was validated in terms of accuracy, precision and linearity and proved to be robust. This CE method is comparable with high performance liquid chromatography (HPLC). This CE method is applicable to the quantitative analysis of Vanillin ando-vanillin in real samples, and the assay results were satisfactory

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