<p>The Late Miocene has been considered one of the most climatically stable periods of the Cenozoic, time span characterized by minor long-term cooling and ice growth. Especially, the Tortonian-Messinian Transition is recognized as a priority for paleoenvironmental reconstruction and climate modelling due to the significant paleoenvironmental changes preceding the Messinian Salinity Crisis (MSC; 5.97-5.33 Ma). Here, we present stable oxygen (&#948;<sup>18</sup>O) and carbon (&#948;<sup>13</sup>C) isotopes measured on benthic and planktonic foraminifera from Potamida section (Crete Island, eastern Mediterranean). The &#948;<sup>18</sup>O results indicate a decoupling between the surface and the bottom water column starting before the Tortonian-Messinian boundary. The difference between planktonic and benthic oxygen isotope signals (&#916;&#948;<sup>18</sup>O) further provides an estimate of the degree of water column stratification during that time. The &#948;<sup>13</sup>C data indicate a generally trend towards lighter values as an excellent illustration of the Late Miocene Carbon Isotope Shift (LMCIS; 7.6-6.6 Ma) due to progressive restriction of the Mediterranean basin, with the exception of the 7.38-7.26 Ma time interval where significantly heavier &#948;<sup>13</sup>C values are documented in both records. Such changes in carbon cycle seem to be most pronounced in the planktonic foraminiferal record (surface waters) through a 6-cycle development indicative of a cyclic productivity pattern during the latest Tortonian. The entire record is substantiated by sea surface temperature (SST) estimates based on TEX<sub>86</sub> biomarker based proxy. The reconstructed SST record shows that a warm phase characterized the late Tortonian sea surface (~27&#8304;C), time followed by a strong, steady cooling starting with earliest Messinian, when the SSTs dropped to values as low as 20&#8304;C. The outcome of the combined stable isotope and biomarker based SST data hint to increased salinity in the surface waters already before the Messinian, while at the Tortonian-Messinian Transition, the conditions in the surface waters changed towards cooler (~24&#8304;C) and normal salinity conditions.</p>