Direct shoot regeneration and cytological studies for date palm (Phoneix dactylifera var pyarum)

2013 ◽  
Vol 24 ◽  
pp. S122-S123
Author(s):  
Mansour Heidarpoor ◽  
Mahmoud Khosroshaheli ◽  
Islam Majidi ◽  
Reza Azizinezhad ◽  
Fereydoon Bondarian
Keyword(s):  
Shoot Regeneration ◽  
Date Palm ◽  
Direct Shoot Regeneration ◽  
Direct Shoot

scholarly journals Investigation on the direct shoot regeneration in date palm CV.Pyarum and possible genetics changes in induced shoots

2020 ◽  
Vol 33 (02) ◽  
pp. 423-430
Author(s):  
Mansoor Heidarpoor ◽  
Mansoor Kalantar ◽  
Mahmoud Khosroshaheli ◽  
Eslam Majidi Hervan
Keyword(s):  
Tissue Culture ◽  
Shoot Regeneration ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Shoot Tips ◽  
Direct Regeneration ◽  
Axillary Shoot ◽  
Direct Shoot Regeneration ◽  
Direct Shoot ◽  
Shoot Meristems

The palm (Phoenix Dactylifera) is one of important trees, and is economically important in south of Iran. Date palm is propagated by the offshoots, number of which is limited. Therefore, adult Date palms produce shoot tips and axillary shoot meristems through the use of a tissue culture. This study was conducted to perform in -vitro tissue culture direct shoot regeneration and determine the best combination of plant regulators and other conditions. To achieve organogenesis and multiplication directly from shoot tips and axillary shoot meristems of Date palm (Phoenix Dactylifera L. Var Pyarum) was used without callus formation. Direct regeneration of vegetative buds minimizes the risk of somaclonal variation among plant regenerates. Results revealed that MS medium supplemented with 4mg/l of kinetin and 3 mg/l of IAA or 2mg/l of BA and 4 mg/l of NAA was the best formation from shoot tip after 16-20 weeks. Subculture per month was evaluated at following conditions: temperature for growth of 27±1°C during the lighted period and 22±1°C during the dark period.

Direct shoot regeneration from leaf explants of Rosa damascena Mill.

2004 ◽  
Vol 40 (2) ◽  
pp. 192-195 ◽  
Author(s):  
Pratap Kumar Pati ◽  
Madhu Sharma ◽  
Anil Sood ◽  
P. S. Ahuja
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Rosa Damascena ◽  
Direct Shoot Regeneration ◽  
Direct Shoot

scholarly journals Improvement of micropropagation protocol of Phalaenopsis sp. for the method of direct shoot regeneration from nodes of floral spikes

2012 ◽  
pp. 141-150
Author(s):  
Marija Markovic ◽  
Milos Tanasic ◽  
Nevena Stojic ◽  
Radivoje Bulatovic ◽  
Marta Jovic ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Coconut Water ◽  
Soy Flour ◽  
Multiplication Rate ◽  
Direct Shoot Regeneration ◽  
Medium Components ◽  
Cultivation In Vitro ◽  
Direct Shoot

This paper succesfully investigated the possibility of modification of the micropropagation protocol of Phalaenopsis sp. with an aim to simplify the procedure and reduce the costs. The obtained results show that some medium components can be succesfully omitted (coconut water, glutamine, 2-morpholinoethanesulfonic acid) and some of them (peptone) can be replaced with a cheaper constituent (soy flour) while preserving the quality of the obtained microplants. The multiplication rate was 7,6 shoots per explant after the period of 150 days of cultivation in vitro. On the same medium 60% of explants were rooted and roots were mostly well developed.

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