scholarly journals Investigation on the direct shoot regeneration in date palm CV.Pyarum and possible genetics changes in induced shoots

2020 ◽  
Vol 33 (02) ◽  
pp. 423-430
Author(s):  
Mansoor Heidarpoor ◽  
Mansoor Kalantar ◽  
Mahmoud Khosroshaheli ◽  
Eslam Majidi Hervan
Keyword(s):  
Tissue Culture ◽  
Shoot Regeneration ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Shoot Tips ◽  
Direct Regeneration ◽  
Axillary Shoot ◽  
Direct Shoot Regeneration ◽  
Direct Shoot ◽  
Shoot Meristems

The palm (Phoenix Dactylifera) is one of important trees, and is economically important in south of Iran. Date palm is propagated by the offshoots, number of which is limited. Therefore, adult Date palms produce shoot tips and axillary shoot meristems through the use of a tissue culture. This study was conducted to perform in -vitro tissue culture direct shoot regeneration and determine the best combination of plant regulators and other conditions. To achieve organogenesis and multiplication directly from shoot tips and axillary shoot meristems of Date palm (Phoenix Dactylifera L. Var Pyarum) was used without callus formation. Direct regeneration of vegetative buds minimizes the risk of somaclonal variation among plant regenerates. Results revealed that MS medium supplemented with 4mg/l of kinetin and 3 mg/l of IAA or 2mg/l of BA and 4 mg/l of NAA was the best formation from shoot tip after 16-20 weeks. Subculture per month was evaluated at following conditions: temperature for growth of 27±1°C during the lighted period and 22±1°C during the dark period.

scholarly journals High frequency direct shoot regeneration from Kazakh commercial potato cultivars

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9447
Author(s):  
Laura S. Abeuova ◽  
Balnur R. Kali ◽  
Aizhan O. Rakhimzhanova ◽  
Sara S. Bekkuzhina ◽  
Shuga A. Manabayeva
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Multiple Shoot ◽  
Potato Cultivars ◽  
Direct Regeneration ◽  
Naphthaleneacetic Acid ◽  
Direct Shoot Regeneration ◽  
Potato Genome ◽  
Direct Shoot

Potato (Solanum tuberosum L.) is the third most economically important crop in the world and has a high nutritional value. In this study, the in vitro culture response of four widely grown in Kazakhstan potato cultivars, Astanalyk, Monument Kunaev, Tokhtar, and Aksor, was investigated using stem and leaf explants. Published protocols were evaluated and optimized to develop a more efficient protocol for the regeneration of plants from local potato cultivars in tissue culture, which is a prerequisite to facilitate potato genome modification. The explants were cultured on solid Murashige and Skoog medium supplemented with different concentrations and combinations of zeatin, 6-benzylaminopurine (BAP), gibberellic acid (GA3), 1-naphthaleneacetic acid (NAA) and indole-3-acetic acid (IAA). The maximum regeneration was induced from the stem internodal explants. A significant effect of the explant source on direct regeneration was confirmed with statistical analysis. The number of shoots obtained from the internode was 10.0 from cv. Aksor followed by cvs. Tokhtar and Astanalyk. The medium DRM-VIII with 1 mg/l zeatin, 0.1 mg/l IAA and 7.0 mg/l GA3 was considered the best for direct shoot regeneration and multiple shoot formation from all cultivars. To conclude, we outline a protocol for direct plant regeneration from four potato cultivars. Our findings suggest commercial cultivars Astanalyk and Aksor are good candidates for developing the genome-edited plants through direct shoot regeneration.

Direct shoot regeneration and cytological studies for date palm (Phoneix dactylifera var pyarum)

2013 ◽  
Vol 24 ◽  
pp. S122-S123
Author(s):  
Mansour Heidarpoor ◽  
Mahmoud Khosroshaheli ◽  
Islam Majidi ◽  
Reza Azizinezhad ◽  
Fereydoon Bondarian
Keyword(s):  
Shoot Regeneration ◽  
Date Palm ◽  
Direct Shoot Regeneration ◽  
Direct Shoot

scholarly journals Direct shoot regeneration from cotyledon, leaf and root of Citrus jambhiri Lush

2020 ◽  
Author(s):  
Priyanka Sharma ◽  
Bidhan Roy
Keyword(s):  
Tissue Culture ◽  
Casein Hydrolysate ◽  
Direct Regeneration ◽  
Direct Shoot Regeneration ◽  
Citrus Jambhiri ◽  
Maintenance Medium ◽  
Rough Lemon ◽  
Rooting Medium ◽  
Cultivated Species ◽  
Direct Shoot

ABSTRACTCitrus jambhiri (Rough lemon) is popularly preferred for rootstock for cultivated species of Citrus. Tissue culture is an appreciable technique for mass-multiplication of plant propagules. In this communication direct regeneration of plantlets of Citrus jambhiri Lush. were obtained from cotyledons, roots and leaves. Most of the cotyledon (96%) enlarged on medium supplemented with 50 mg/L of casein hydrolysate. Few of those enlarged cotyledons responded to direct regeneration of shoots. Maximum shoot per responded cotyledon was 32. Conversely, the health of the plantlets were poor with semi-cylindrical leaves. Most of them dried on maintenance medium or on rooting medium ad died. Plantlets regenerated on medium supplemented with IAA in combination with IBA were healthy and they established on maintenance medium and rooted on rooting medium. Direct regeneration was also obtained from leaf on MS medium supplemented with 0.50 mg/L of dicamba. Our finding concluded that tissue culture tools may be used for direct regeneration of plantlets from different explants of C. jambhiri to obtained true-to-type plant propagules.

Direct shoot regeneration from leaf explants of Rosa damascena Mill.

2004 ◽  
Vol 40 (2) ◽  
pp. 192-195 ◽  
Author(s):  
Pratap Kumar Pati ◽  
Madhu Sharma ◽  
Anil Sood ◽  
P. S. Ahuja
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Rosa Damascena ◽  
Direct Shoot Regeneration ◽  
Direct Shoot

scholarly journals Improvement of micropropagation protocol of Phalaenopsis sp. for the method of direct shoot regeneration from nodes of floral spikes

2012 ◽  
pp. 141-150
Author(s):  
Marija Markovic ◽  
Milos Tanasic ◽  
Nevena Stojic ◽  
Radivoje Bulatovic ◽  
Marta Jovic ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Coconut Water ◽  
Soy Flour ◽  
Multiplication Rate ◽  
Direct Shoot Regeneration ◽  
Medium Components ◽  
Cultivation In Vitro ◽  
Direct Shoot

This paper succesfully investigated the possibility of modification of the micropropagation protocol of Phalaenopsis sp. with an aim to simplify the procedure and reduce the costs. The obtained results show that some medium components can be succesfully omitted (coconut water, glutamine, 2-morpholinoethanesulfonic acid) and some of them (peptone) can be replaced with a cheaper constituent (soy flour) while preserving the quality of the obtained microplants. The multiplication rate was 7,6 shoots per explant after the period of 150 days of cultivation in vitro. On the same medium 60% of explants were rooted and roots were mostly well developed.

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