Direct shoot regeneration from leaf explants of Rosa damascena Mill.

Potato (Solanum tuberosum L.) is the third most economically important crop in the world and has a high nutritional value. In this study, the in vitro culture response of four widely grown in Kazakhstan potato cultivars, Astanalyk, Monument Kunaev, Tokhtar, and Aksor, was investigated using stem and leaf explants. Published protocols were evaluated and optimized to develop a more efficient protocol for the regeneration of plants from local potato cultivars in tissue culture, which is a prerequisite to facilitate potato genome modification. The explants were cultured on solid Murashige and Skoog medium supplemented with different concentrations and combinations of zeatin, 6-benzylaminopurine (BAP), gibberellic acid (GA3), 1-naphthaleneacetic acid (NAA) and indole-3-acetic acid (IAA). The maximum regeneration was induced from the stem internodal explants. A significant effect of the explant source on direct regeneration was confirmed with statistical analysis. The number of shoots obtained from the internode was 10.0 from cv. Aksor followed by cvs. Tokhtar and Astanalyk. The medium DRM-VIII with 1 mg/l zeatin, 0.1 mg/l IAA and 7.0 mg/l GA3 was considered the best for direct shoot regeneration and multiple shoot formation from all cultivars. To conclude, we outline a protocol for direct plant regeneration from four potato cultivars. Our findings suggest commercial cultivars Astanalyk and Aksor are good candidates for developing the genome-edited plants through direct shoot regeneration.

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Direct shoot regeneration from leaf explants of Jatropha curcas in response to thidiazuron and high copper contents in the medium

Biologia Plantarum ◽

10.1007/s10535-010-0066-3 ◽

2010 ◽

Vol 54 (2) ◽

pp. 369-372 ◽

Cited By ~ 35

Author(s):

V. Khurana-Kaul ◽

S. Kachhwaha ◽

S. L. Kothari

Keyword(s):

Shoot Regeneration ◽

Jatropha Curcas ◽

Leaf Explants ◽

Direct Shoot Regeneration ◽

High Copper ◽

Direct Shoot

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Direct Shoot Organogenesis from Lycium chinense Miller Leaf Explants and Assessment of Genetic Stability Using ISSR Markers

Agronomy ◽

10.3390/agronomy11030503 ◽

2021 ◽

Vol 11 (3) ◽

pp. 503

Author(s):

Woo-Suk Jung ◽

Ill-Min Chung ◽

Seung-Hyun Kim ◽

Hee-Yeon Chi ◽

Chang Yeon Yu ◽

...

Keyword(s):

Shoot Regeneration ◽

Growth Regulator ◽

Genetic Stability ◽

Leaf Explants ◽

Lycium Chinense ◽

Regeneration System ◽

Direct Shoot Regeneration ◽

Genetic Traits ◽

Lycium Chinense Miller ◽

Direct Shoot

An efficient in vitro direct shoot regeneration system has been described for Lycium chinense Miller using leaf explants. Influence of various parameters such as growth regulator concentration, explant type, effect of basal salt type, Murashige and Skoog (1962) medium (MS), Schenk and Hildebrandt (1972) medium (SH), Gamborg et al. (1968) medium (B5), and carbon sources (sucrose, maltose, and fructose) on the regenerating shoots has been studied. Micromorphological studies and genetic fidelity of regenerated shoots were assessed and compared with those of the donor plants. Among the different concentrations of plant growth regulator (PGRs) tested, MS supplemented with lower concentration of 6-benzylaminopurine (BAP) (0.5 mgL−1) and thidiazuron (TDZ) (0.5 mgL−1) increased the frequency of shoot. Comparatively, indole-3-butyric acid (IBA) was more effective in the regeneration and growth of the root system. A higher number of root formation (6.67 ± 1.25) was observed when the rooting medium comprised half-strength MS salts supplemented with 3% sucrose. The surviving plantlets were gradually transferred to the greenhouse and natural soil. More than 90% of the plantlets survived and matured within 85 days. Similarity in the band patterns produced by inter simple sequence repeat (ISSR) primers confirmed the genetic stability and uniformity between the regenerated and donor plants. The present optimized direct shoot regeneration system may be useful for mass propagation and improving the genetic traits in L. chinense.

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Direct shoot regeneration from leaf explants of Spilanthes acmella

Biologia Plantarum ◽

10.1007/s10535-008-0068-6 ◽

2008 ◽

Vol 52 (2) ◽

pp. 334-338 ◽

Cited By ~ 22

Author(s):

K. V. Saritha ◽

C. V. Naidu

Keyword(s):

Shoot Regeneration ◽

Leaf Explants ◽

Direct Shoot Regeneration ◽

Spilanthes Acmella ◽

Direct Shoot

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Direct shoot regeneration from excised leaf explants of in vitro grown seedlings of Alstroemeria L.

Plant Cell Reports ◽

10.1007/s002990050317 ◽

1997 ◽

Vol 16 (11) ◽

pp. 770-774 ◽

Cited By ~ 30

Author(s):

H. S. Lin ◽

M. J. De Jeu ◽

E. Jacobsen

Keyword(s):

Shoot Regeneration ◽

Leaf Explants ◽

Direct Shoot Regeneration ◽

Direct Shoot

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In vitro propagation of black cumin (Nıgella satıva L.) plants

Genetika ◽

10.2298/gensr2101295u ◽

2021 ◽

Vol 53 (1) ◽

pp. 295-303

Author(s):

Hüseyin Uysal

Keyword(s):

Success Rate ◽

Shoot Regeneration ◽

Callus Formation ◽

Leaf Explants ◽

Shoot Formation ◽

Stem Explants ◽

Direct Shoot Regeneration ◽

Black Cumin ◽

Direct Shoot

This study was carried out to determine in vitro development using Black cumin leaf and stem explants. ?ameli black cumin variety was used as plant material. Five different nutrient mediums (1. LS2.5, 2. MS, 3. MS + 0.5 mg.l-1 IAA, 4. MS + 0.5 mg.l-1 BAP, 5. MS + 0.5 mg.l-1 IAA + 0.5 mg.l-1 BAP) containing 30 g sugar were used in this study. As a result of the research, 100% callus formation was detected in the stem explants cultured in the number 1 and number 5 mediums. These were followed by stem explants cultured in medium 4 with a success rate of 96%. Of this rate, 66% was shoot formation, and 30% was callus formation. Direct shoot regeneration was performed only on stem explants cultured in mediums 4 and 3, with a 66% success rate in medium four and a 36% success rate in medium 3. The highest plant regenerations from calluses were gained from stem explants (273.3%) in medium 4, followed by calluses gained from leaf explants (262.5%) in the same medium. These were followed by cultures in medium 3, with calluses derived from stem explants (255%) and leaf explants (150%). No plant regeneration was determined from calluses gained in the medium 1. Thus it is evident that high auxin content and auxin-cytokinin balanced mediums encouraged callus formation in the black cumin plants. The addition of only IAA or BAP to the medium promoted shoot formation in the stem explants, but direct shoot regeneration was not thereby achieved from the leaf explants. These results show that, for in vitro clonal propagation studies done on black cumin plants, a high auxin containing medium is preferable if the aim is callus formation. If the aim is direct shoot regeneration, BAP or other cytokinin-containing medium is preferred.

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