Diagnostic performance of tests based on Trypanosoma cruzi excreted–secreted antigens in an endemic area for Chagas' disease in Bolivia

2007 ◽  
Vol 57 (2) ◽  
pp. 229-232 ◽  
Author(s):  
Carlos Rodrigo Zarate-Blades ◽  
Nelly Bladés ◽  
Marilda Savoia Nascimento ◽  
José Franco da Silveira ◽  
Eufrosina Setsu Umezawa
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Diagnostic Performance ◽  
Endemic Area ◽  
Secreted Antigens

scholarly journals Immunoblotting analyses using two-dimensional gel electrophoresis of Trypanosoma cruzi excreted-secreted antigens

2004 ◽  
Vol 37 (6) ◽  
pp. 454-459
Author(s):  
Adriano Gomes Silva ◽  
Elisangela Paula Silveira-Lacerda ◽  
Jair Pereira Cunha-Júnior ◽  
Maria Aparecida de Souza ◽  
Silvio Favoreto Junior
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Gel Electrophoresis ◽  
Complex Mixture ◽  
Chronic Phase ◽  
Disease Diagnosis ◽  
Basic Knowledge ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Secreted Antigens

Trypanosoma cruzi trypomastigotes excrete-secrete a complex mixture of antigenic molecules. This antigenic mixture denominated trypomastigote excreted-secreted antigens contains a 150-160 kDa band that shows excellent performance in Chagas' disease diagnosis by immunoblotting. The present study partially characterized by two-dimensional gel electrophoresis the immunoreactivity against the 150-160kDa protein using sera samples from chagasic patients in different phases of the disease. Trypomastigote excreted-secreted antigen preparations were subjected to high-resolution two-dimensional (2D) gel electrophoresis followed by immunoblotting with sera from chagasic and non-chagasic patients. The 150-160kDa protein presented four isoforms with isoelectric focusing ranging from 6.2 to 6.7. The four isoforms were recognized by IgM from acute phase and IgG from chronic phase sera of chagasic patients. The 150-160kDa isoform with IF of approximately 6.4 became the immunodominant spot with the progression of the disease. No cross-reactivity was observed with non-chagasic or patients infected with Leishmania sp. In this study we provide basic knowledge that supports the validation of trypomastigote excreted-secreted antigens for serological diagnosis of Chagas' disease.

scholarly journals Genotyping of Trypanosoma cruzi in a hyper-endemic area of Colombia reveals an overlap among domestic and sylvatic cycles of Chagas disease

2014 ◽  
Vol 7 (1) ◽  
pp. 108 ◽  
Author(s):  
Ana Mejía-Jaramillo ◽  
Luz Agudelo-Uribe ◽  
Juan Dib ◽  
Sylvia Ortiz ◽  
Aldo Solari ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Endemic Area

scholarly journals Biological behavior of different Trypanosoma cruzi isolates circulating in an endemic area for Chagas disease in the Gran Chaco region of Argentina

Acta Tropica ◽  
2012 ◽  
Vol 123 (3) ◽  
pp. 196-201 ◽  
Author(s):  
Paula G. Ragone ◽  
Cecilia Pérez Brandán ◽  
Angel M. Padilla ◽  
Mercedes Monje Rumi ◽  
Juan J. Lauthier ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Endemic Area ◽  
Biological Behavior ◽  
Gran Chaco ◽  
Chaco Region

Antibody isotypes profiles against Trypanosoma cruzi acidic antigens in two Amerindian populations from a Chagas' disease endemic area

Acta Tropica ◽  
1994 ◽  
Vol 58 (2) ◽  
pp. 105-114 ◽  
Author(s):  
Claudia C. Motran ◽  
Horacio M. Serra ◽  
Susana E. Gea ◽  
Carlos M. Vullo ◽  
Elsa Vottero-Cima
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Endemic Area ◽  
Antibody Isotypes

scholarly journals Purified Excreted-Secreted Antigens from Trypanosoma cruzi Trypomastigotes as Tools for Diagnosis of Chagas' Disease

2006 ◽  
Vol 44 (2) ◽  
pp. 291-296 ◽  
Author(s):  
M. Berrizbeitia ◽  
M. Ndao ◽  
J. Bubis ◽  
M. Gottschalk ◽  
A. Ache ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Secreted Antigens

scholarly journals Excretory-Secretory Antigens of Trypanosoma cruzi Are Potentially Useful for Serodiagnosis of Chronic Chagas' Disease

2001 ◽  
Vol 8 (5) ◽  
pp. 1024-1027 ◽  
Author(s):  
Mineo Nakazawa ◽  
Daniela S. Rosa ◽  
Valéria R. A. Pereira ◽  
Milena O. Moura ◽  
Veridiana C. Furtado ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Sensitivity And Specificity ◽  
Immunosorbent Assay ◽  
High Sensitivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chronic Chagas Disease ◽  
Secreted Antigens

ABSTRACT The reactivities of sera from chronic chagasic patients against the trypomastigote excreted-secreted antigens (TESA) of Trypanosoma cruzi strains with different biodemes were analyzed by TESA-blot and TESA–enzyme-linked immunosorbent assay (ELISA). Although both tests presented high sensitivity and specificity, TESA-ELISA is more appropriate for screening a larger number of samples.

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