In vitro antifungal susceptibility and molecular identity of 99 clinical isolates of the opportunistic fungal genus Curvularia

Investigations on antifungal drug susceptibility were carried out on 90 clinical isolates of Trichophyton rubrum, and Trichophyton mentagrophytes with four antifungal drugs, namely griseofulvin, fluconazole, itraconazole and terbinafine as suggested by National Committee for Clinical Laboratory Standards (NCCLS) M27–A (1997) document by broth macrodilution method to standardize in vitro antifungal susceptibility testing and to find out the Minimum Inhibitory Concentration (MIC) of the drugs. In this study, terbinafine was found to be the most efficient drug for all isolates. Terbinafine had the lowest MIC range of 0.001 g/ml to 0.09 g/ml and MIC50 was low at 0.005 g/ml and MIC90 was also low at 0.04 g/ml against T.rubrum; and MIC range of 0.001μg/ml to 0.19μg/ml with a MIC50 of 0.01μg/ml and MIC90 at 0.09μg/ml against T.mentagrophytes. Itraconazole showed antifungal activity superior to that of fluconazole, with a MIC range of 0.04g/ml to 1.56g/ml, with MIC50 at 0.19μg/ml and MIC90 at 1.56g/ml against T.rubrum; and MIC range of 0.04μg/ml to 1.56μg/ml, with MIC50 at 0.19μg/ml and MIC90 at 0.78μg/ml against T.mentagrophytes. Griseofulvin appears to be still a potent drug for management of dermatophytoses. Griseofulvin had a MIC range of 0.15g/ml to 5.07 g/ml with MIC50 at1.26 g/ml and MIC90 at 2.53 g/ml against T.rubrum; and MIC range of 0.31μg/ml to 5.07μg/ml with MIC50 at 1.26μg/ml and MIC90 at 2.53μg/ml against T.mentagrophytes. Fluconazole showed a high MIC range of 0.19 g/ml to 50 g/ml and MIC50 was high at 1.56g/ml and MIC90 was also high at 12.5 g/ml against T.rubrum; and a high MIC range of 0.09μg/ml to 25.0μg/ml, with MIC50 at 1.56μg/ml and MIC90 at 12.5μg/ml towards T.mentagrophytes. The technique was found to be easy to perform and reliable with consistent results.

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In vitro antifungal susceptibility patterns of planktonic and sessile Candida kefyr clinical isolates

Medical Mycology ◽

10.1093/mmy/myx062 ◽

2017 ◽

Vol 56 (4) ◽

pp. 493-500 ◽

Cited By ~ 5

Author(s):

Fruzsina Nagy ◽

Aliz Bozó ◽

Zoltán Tóth ◽

Lajos Daróczi ◽

László Majoros ◽

...

Keyword(s):

Antifungal Susceptibility ◽

Clinical Isolates ◽

Candida Kefyr ◽

In Vitro Antifungal Susceptibility ◽

Susceptibility Patterns

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In Vitro Antifungal Susceptibility and Molecular Characterization of Clinical Isolates of Fusarium verticillioides (F. moniliforme) and Fusarium thapsinum

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00176-08 ◽

2008 ◽

Vol 52 (6) ◽

pp. 2228-2231 ◽

Cited By ~ 28

Author(s):

Mónica Azor ◽

Josepa Gené ◽

Josep Cano ◽

Deanna A. Sutton ◽

Annette W. Fothergill ◽

...

Keyword(s):

Active Drug ◽

Fusarium Verticillioides ◽

Antifungal Susceptibility ◽

Antifungal Drugs ◽

Clinical Isolates ◽

Microdilution Method ◽

Fusarium Thapsinum ◽

In Vitro Antifungal Susceptibility

ABSTRACT A microdilution method was used to test 11 antifungal drugs against clinical isolates of Fusarium thapsinum and three different phylogenetic clades of Fusarium verticillioides that were characterized by sequencing a region of the β-tubulin gene. Terbinafine was the most-active drug against both species, followed by posaconazole against F. verticillioides.

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Species Distribution and In Vitro Antifungal Susceptibility Patterns of 75 Clinical Isolates of Fusarium spp. from Northern Italy

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00272-08 ◽

2008 ◽

Vol 52 (7) ◽

pp. 2683-2685 ◽

Cited By ~ 56

Author(s):

Anna Maria Tortorano ◽

Anna Prigitano ◽

Giovanna Dho ◽

Maria Carmela Esposto ◽

Claudia Gianni ◽

...

Keyword(s):

Species Distribution ◽

Fusarium Verticillioides ◽

Antifungal Susceptibility ◽

Northern Italy ◽

Molecular Methods ◽

Clinical Isolates ◽

Fusarium Spp ◽

In Vitro Antifungal Susceptibility ◽

Susceptibility Patterns

ABSTRACT Fusarium isolates from 75 Italian patients were identified by molecular methods, and their susceptibilities to antifungals were tested in vitro. Fusarium verticillioides was the species most frequently isolated from deep-seated infections, and F. solani was the species most frequently isolated from superficial infections. F. solani isolates showed high azole MICs, while F. verticillioides isolates showed low posaconazole MICs.

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Azole Antifungal Resistance in Candida albicans and Candida glabrata Isolated from Vulvovaginal Candidiasis Patients

Archives of Clinical Infectious Diseases ◽

10.5812/archcid.106360 ◽

2021 ◽

Vol 16 (2) ◽

Author(s):

Niloofar Deravi ◽

Mobina Fathi ◽

Seyede Nadia Tabatabaeifar ◽

Parichehr Pooransari ◽

Bahram Ahmadi ◽

...

Keyword(s):

Candida Albicans ◽

Candida Glabrata ◽

Antifungal Susceptibility ◽

Vulvovaginal Candidiasis ◽

Clinical Isolates ◽

In Vitro Activity ◽

Normal Women ◽

In Vitro Antifungal Susceptibility ◽

Iranian Patients

Background: Vulvovaginal candidiasis (VVC) is the most frequent fungal disorder in healthy and normal women. Objectives: The aim of this study was to evaluate the in vitro antifungal susceptibility of clinical isolates Candida albicans and Candida glabrata, the two most common candida species in Iranian patients with VVC. Methods: One hundred and eight clinical isolates of candida, including; C. albicans (n = 77) and C. glabrata: (n = 31) were isolated from the 108 patients with VVC. The in vitro activity of caspofungin (CAS), amphotericin B (AMB), voriconazole (VRC), itraconazole (ITC), fluconazole (FLC), and nystatin (NYS) were determined according to the CLSI M27-A3 and CLSI M27-S4. Results: Our results were shown 8 (25.8 %) and 6 (7.8 %) C. glabrata and C. albicans isolates resistance to FLU, respectively. Furthermore, resistance to VRC and ITC were observed in 8.4%, and 3.7% of all isolates, and six isolates (5.6%) had intermediate MIC to CAS. Conclusions: We reported 8 (25.8 %) and 6 (7.8 %) C. glabrata and C. albicans isolates resistance to FLU, respectively. Furthermore, resistance to VRC and ITC were observed in 8.4% and 3.7% of all isolates, respectively.

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In vitro antifungal susceptibility of griseofulvin, fluconazole, itraconazole and terbinafine against clinical isolates of Trichophyton rubrum and Trichophyton mentagrophytes

Journal of Universal College of Medical Sciences ◽

10.3126/jucms.v1i4.9569 ◽

2014 ◽

Vol 1 (4) ◽

pp. 26-28

Author(s):

KR Reddy ◽

SR Reddy

Keyword(s):

Trichophyton Rubrum ◽

Clinical Laboratory ◽

Antifungal Susceptibility ◽

Trichophyton Mentagrophytes ◽

Drug Susceptibility ◽

Antifungal Drugs ◽

Clinical Isolates ◽

National Committee ◽

In Vitro Antifungal Susceptibility

Investigations on antifungal drug susceptibility were carried out on 90 clinical isolates of Trichophyton rubrum, and Trichophyton mentagrophytes with four antifungal drugs, namely griseofulvin, fluconazole, itraconazole and terbinafine as suggested by National Committee for Clinical Laboratory Standards (NCCLS) M27A (1997) document by broth macrodilution method to standardize in vitro antifungal susceptibility testing and to find out the Minimum Inhibitory Concentration (MIC) of the drugs. In this study, terbinafine was found to be the most efficient drug for all isolates. Terbinafine had the lowest MIC range of 0.001 g/mlto 0.09 g/ml and MIC50 was low at 0.005 g/ml and MIC90 was also low at 0.04 g/ml against T. rubrum; and MIC range of 0.001pg/ml to 0.19pg/ml with a MIC50 of 0.01pg/ml and MIC90 at 0.09 pg/ml against T. mentagrophytes. Itraconazole showed antifungal activity superior to that of fluconazole, with a MIC range of 0.04g/ml to l.56g/ml, with MIC50 at 0.19pg/m land MIC90 at l.56g/ml against T. rubrum; and MIC range of 0.04.g/ml to 1.56pg/ml, with MIC50 at 0.19pg/ml and MIC90 at 0.78pg/ml against T. mentagrophytes. Griseofulvin appears to be still a potent drug for management of dermatophytoses. Griseofulvin had a MIC range of 0.15g/ml to 5.07 g/ml with MIC50 at l.26 g/ml and MIC90 at 2.53 g/ml against T. rubrum; and MIC range of 0.31pg/ml to 5.07pg/ml with MIC50 at 1.26pg/ml and MIC90 at 2.53pg/ml against T. mentagrophytes. Fluconazole showed a high MIC range of 0.19 g/ml to 50 g/ml and MIC50 was high at 1.56g/ml and MIC90 was also high at 12.5 g/ml against T. rubrum; and a high MIC range of 0.09pg/ml to 25.0pg/ml, with MIC50 at 1.56pg/ml and MIC90 at 12.5pg/ml towards T. mentagrophytes. The technique was found to be easy to perform and reliable with consistent results.DOI: http://dx.doi.org/10.3126/jucms.v1i4.9569 Journal of Universal College of Medical Sciences (2013) Vol.1 No.04: 26-28

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